口腔护理产品美白功效测试实验室方法进展

doi:10.3969/j.issn.2097-2806.2024.10.014

摘要/Abstract

摘要：

口腔护理产品的美白功效指口腔护理产品通过去除牙齿表面的污渍、色素和牙菌斑，改善口腔环境所达到的美白效果。从口腔护理市场容量占比可见，美白功效始终是消费者的核心诉求。国内外关于口腔护理产品美白功效虚假宣传、夸大功效的报道层出不穷，引发了社会的高度关注，也影响着口腔护理行业的进一步发展。因此，建立一套完整的口腔护理产品美白功效测试实验室方法具有重要意义，不仅可满足市场需求，而且有助于提高美白功效宣称产品的科学监管水平。为推动口腔护理产品美白功效测评方法的标准化，综述了国内外口腔护理产品美白功效实验室方法的设计原理、实验程序、检测技术及结果分析，从模型制备、染色方式、美白过程及应用评价四个维度进行了对比。以期提出科学严谨的标准化建议，为建立一套完整的口腔美白护理产品功效测试实验室方法提供支持。

关键词: 牙齿, 美白, 外源性着色, 体外研究

Abstract:

The whitening effect of oral care products refers to the whitening effect achieved by removing stains, pigments and plaque on the surface of teeth. Commonly used oral whitening care products include whitening toothpaste, mouthwash, whitening tooth strips, whitening gels, etc. It is evident that whitening effect remains the primary concern of consumers from the market share of oral care products. At home and abroad, there are numerous reports of false advertising and exaggerated efficacy of oral care products for whitening, which trigger high social attention and affect the development of oral care industry. Therefore, the establishment of a complete efficacy evaluation system not only meets the market demand, but also helps to improve the scientific supervision level of products with whitening efficacy claims. The design principles, experimental procedures, detection techniques and result analyses of laboratory methods for assessing the whitening efficacy of oral care products are reviewed, focusing on both domestic and international approaches. The four dimensions of model preparation, staining method, whitening process and application evaluation are compared to standardize the whitening efficacy assessment methods for oral care products. Scientific and rigorous standardization suggestions are put forward, which provide data support for the establishment of a complete set of laboratory methods for efficacy testing of oral whitening care products.

Key words: teeth, whitening, exogenous staining, in vitro study

中图分类号:

TQ658

乔翰, 吴孟轩, 刘子航, 程树军. 口腔护理产品美白功效测试实验室方法进展[J]. 日用化学工业（中英文）, 2024, 54(10): 1260-1267.

Han Qiao, Mengxuan Wu, Zihang Liu, Shujun Cheng. Advances in laboratory methods for testing whitening efficacy of oral care products[J]. China Surfactant Detergent & Cosmetics, 2024, 54(10): 1260-1267.

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参考文献	刷牙方式	刷磨次数（往返为一次）	刷牙力	牙膏与水的比例
[28]	V8交叉刷牙机（V8 cross brushing machine）	800次	150 g	25 g∶40 mL（蒸馏水）
[33]	自制刷牙机	100 000次（320 次/min）
[24]	自制刷牙机，使用中等硬度的Exton纤维牙刷，在使用前已在水中浸泡16 h	6~16 h（90 次/min）	64~76 g	1∶2
[29]	BIOPDI自动刷牙机，软牙刷	5 d （90 次/d）	150 g	1∶3（人造唾液）
[30]	交叉刷牙机（Cross brushing machine），直刷头	1 000次（200 次/min）	150 g
[31]	手工刷牙，软刷毛，水平刷动	168 min		1∶3

研究	覆盖方式	处理时间	附加因素
[36]	在牙齿颊面上均匀覆盖凝胶，结束后不洗去	每天2次，每次3 min，28 d
[37]	在牙齿颊面上均匀覆盖凝胶，结束后蒸馏水洗去	每疗程3次，每次15 min，共 3疗程，疗程间隔72 h
[35]	样品顶部放置增白条，测试间隔期间，将样本在流动的蒸馏水中冲洗1 min并浸入37 ℃蒸馏水中直到下一次处理	2 次/天，每次30 min，共21 d
[38]	使用乙酸乙烯酯乙酯板和真空机为每个样品制作一个厚度为1 mm的托盘。凝胶被施加到托盘中，并将托盘每天放置在每个样品上。完成每日漂白程序后，用去离子蒸馏水冲洗样品5 s	每天6 h，21 d
[42]	涂敷试剂+光照刺激	每次15 min，4 次	15 min的前8 min用卤素灯照射/前30 s用激光照射

研究	浸泡时间	是否搅动
[51]	每天2 min，共21 d
[52]	每天4 min，共56 d
[53]	每次2 min，每天3 次，共30 d
[54]	1 min/d，每次浸泡前后10 s清洗
[55]	连续24 h	每3 h搅动一次维持均匀