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日用化学工业（中英文）

日用化学工业 ›› 2014, Vol. 44 ›› Issue (11): 628-630.doi: 10.13218/j.cnki.csdc.2014.11.140

• 开发与应用 • 上一篇    下一篇

化妆品微生物检验影响因素的探讨

谢小保, 杨秀茳, 孙廷丽, 李彩玲, 文霞   

  1. 广东省微生物研究所 广东省微生物分析检测中心 华南应用微生物国家重点实验室 广东省菌种保藏与应用重点实验室,广东 广州 510070
  • 收稿日期:2014-04-21 出版日期:2014-11-22 发布日期:2019-04-08
  • 作者简介:谢小保(1966-),男,江西人,研究员,硕士,电话:(020)37656986,E-mail:xiaobaoxie@126.com。
  • 基金资助:
    广东省科技计划资助项目(2012B040301043)

Study on affecting factors of microbiological inspection of cosmetics

XIE Xiao-bao, YANG Xiu-jiang, SUN Ting-li, LI Cai-ling, WEN Xia   

  1. Guangdong Institute of Microbiology,Guangdong Detection Center of Microbiology,State Key Laboratory of Applied Microbiology Southern China,Guangdong Provincial Key Laboratory of Microbial Culture Collection and Application,Guangzhou,Guangdong 510070,China
  • Received:2014-04-21 Online:2014-11-22 Published:2019-04-08

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摘要： 通过设置加中和剂和不加中和剂2个实验组,按照《化妆品卫生规范》(2007年版)中规定的平板培养法对化妆品中微生物进行检验,对2组实验的菌落检测情况进行比较分析。结果显示:加入中和剂的实验组,化妆品稀释10倍可检出菌落;不加入中和剂的实验组,18种化妆品稀释100倍可检出菌落,2种膏霜类化妆品稀释1 000倍可检出菌落。表明中和剂能够减少检测时化妆品的稀释倍数;另外,氯化三苯基四氮唑(TTC)对化妆品中的细菌有抑制作用,TTC影响检测结果的准确性。建议在化妆品稀释液中添加中和剂,降低培养基中TTC浓度,在标准中增加方法验证试验,以70%回收率作为判定试验有效性的依据。

关键词: 化妆品, 微生物检验, 防腐剂

Abstract: Via setting up of two experimental groups,in which one composed of neutralizing agent and another without it,microbial inspection was carried out according to plate culture method that specified in China Hygienic Standard for Cosmetics.Experimental results on the colony detection of the two groups were compared.In the group with neutrazing agent added,10-fold dilution of cosmetics can be detected colonies,while in the group without neutralizing agent added,100-fold dilution of 18 cosmetics can be detected colonies,and 1 000-fold dilution of 2 creams can be detected colonies.Such results showed that the neutralizing agent can reduce the dilution of cosmetics when detection.Further,it was also found that TTC (2,3,5-triphenyl terazolium chloride) can inhibit the growth of bacteria in the cosmetics,so that TTC will affect the accuracy of the test results.It is recommended that verification test should be incorporated in the test standard;neutralizing agent should be added in the diluted solution of the cosmetics to reduce concentration of TTC in the culture medium;and 70% recovery of the test bacteria should be taken as the criteria for determining validity of the test.

Key words: cosmetics, microbial inspection, preservatives

中图分类号: 

  • TQ658