关键词: 气相色谱-质谱/质谱, 固相萃取, 化妆品, 酚类物质

Abstract:

A method based on gas chromatography-tandem mass spectrometry （GC-MS/MS） was developed for the analysis of 5 phenolics compounds （phenol, hydroquineone, resorcinol, 4-butylresorciol, phenylethyl resorciol） in cosmetics. The phenolics compounds were extracted from cosmetics by supersonic extraction with 0.03 mol/L potassium dihydrogen phosphate solution, purified by HLB solid phase extraction column, and analyzed via GC-MS/MS after filtration with organic filtration membrane （0.22 μm）. Separation was performed on a SH-Rtx-1701MS chromatographic column （30 m×0.25 mm×0.25 μm） by GC-MS/MS with an electrospray ionization （EI） source in the MRM mode. The external standard method was used for quantitative determination. The 5 phenolics compounds can be analyzed within 10 min under the optimum conditions, and it exhibits a good linearity with the standard correlation coefficients （R²） of not less than 0.999 in their respective concentration ranges of 5-200 μg/L. The limited of determination （LODs） are 1.5-3.0 μg/L and the limits of quantitation （LOQs） are 5.0-10.0 μg/L, respectively. The recoveries at three standards are 73.5%-111.8%, and the relative standard deviations （RSDs） are between 1.0% and 11.1% （n=6）. The proposed method is accurate, reliable, simple and convenient, and is suitable for determination of phenol whitening active components in cosmetics.

Key words: gas chromatography tandem mass spectrometry （GC-MS/MS）, solid phase extractions, cosmetics, phenolic compounds （phenols）