高效液相色谱法测定唇用化妆品中对位红含量

doi:10.3969/j.issn.1001-1803.2019.06.012

日用化学工业 ›› 2019, Vol. 49 ›› Issue (6): 415-418.doi: 10.3969/j.issn.1001-1803.2019.06.012

• 分析与检测 • 上一篇

高效液相色谱法测定唇用化妆品中对位红含量

侯广月,周莉莉(),卞英芳,许士明,杜营,邹惠玲

山东省产品质量检验研究院国家加工食品质量监督检验中心（山东）,山东济南 250102

收稿日期:2018-10-31 修回日期:2019-05-30 出版日期:2019-06-22 发布日期:2019-06-24
通讯作者: 周莉莉
作者简介:侯广月（1985-）,女,山东人,高级工程师,博士,电话：（0531）89701907,E-mail： jsjzhanzheng@126.com。
基金资助:
国家标准委员会计划项目资助(20160677-T-607);国家质量监督检验检疫总局科技计划项目资助(2017QK077);山东省标准化项目资助(2017-1-052)

Determination of para red in lip cosmetics by high performance liquid chromatography

HOU Guang-yue,ZHOU Li-li(),BIAN Ying-fang,XU Shi-ming,DU Ying,ZOU Hui-ling

National Supervision and Inspection Center for Processed Food Quality（Shandong）,Shandong Product Quality Inspection Research Institute, Jinan, Shandong 250102, China

Received:2018-10-31 Revised:2019-05-30 Online:2019-06-22 Published:2019-06-24
Contact: Li-li ZHOU

摘要/Abstract

摘要：

建立了一种快速检测唇用化妆品中对位红含量的高效液相色谱分析方法。样品经三氯甲烷分散后,用乙腈进行超声提取,过滤后,采用Waters SunFire C₁₈色谱柱（4.6 mm×250 mm,5 μm）分离,以乙腈-水溶液（体积比为85∶15）为流动相进行等度洗脱,流速为1.0 mL/min,柱温30 ℃,二极管阵列检测器检测,外标法定量。结果表明,对位红在0.1~10 mg/L质量浓度范围内线性关系良好,相关系数为0.999 9,方法检出限为0.20 μg/g,定量限为0.60 μg/g,3组加标浓度的平均回收率为94.3%~103.8%。方法操作简便、快速准确、重现性好,适用于唇用化妆品中对位红的测定。

关键词: 化妆品, 高效液相色谱法, 对位红

Abstract:

A high performance liquid chromatography（HPLC）method for the rapid determination of para red in lip cosmetics was established. The sample was dispersed by trichloromethane, ultrasonically extracted with acetonitrile, filtered, separated by a Waters SunFire C₁₈ column（4.6 mm×250 mm, 5 μm）, and eluted equivalently with acetonitrile-water solution（volume ratio 85:15）as mobile phase. The elution was performed at a flow rate of 1.0 mL/min and the column temperature was set at 30 ℃. The analysis was done using photo-diode array detector. External standard approach was used for quantification. The results indicate that para red has a good linear relationship in the range of 0.1-10 mg/L and the correlation coefficient is 0.999 9. The limit of detection（LOD）is 0.20 μg/g, while the limit of quantification（LOQ）is within 0.60 μg/g. The average recoveries were in the range of 94.3%-103.8% at three spiked concentrations. The method is simple, rapid, accurate and reproducible, and is suitable for the determination of para red in lip cosmetics.

Key words: cosmetics, high performance liquid chromatography, para red

中图分类号:

TQ658

侯广月,周莉莉,卞英芳,许士明,杜营,邹惠玲. 高效液相色谱法测定唇用化妆品中对位红含量[J]. 日用化学工业, 2019, 49(6): 415-418.

HOU Guang-yue,ZHOU Li-li,BIAN Ying-fang,XU Shi-ming,DU Ying,ZOU Hui-ling. Determination of para red in lip cosmetics by high performance liquid chromatography[J]. China Surfactant Detergent & Cosmetics, 2019, 49(6): 415-418.

图/表 4

图 1

表 1

表 2

图 2

参考文献 17

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待测物	加入量/（mg·L^-1）	日内测定浓度/（mg·L^-1）	日内RSD/%	日内RE/%	日间测定浓度/（mg·L^-1）	日间RSD/%	日间RE/%
对位红	0.10	0.103±0.007	6.57	2.67	0.103±0.007	6.61	2.50
	0.50	0.486±0.008	1.61	-2.90	0.483±0.008	1.63	-3.43
	10.00	9.915±0.051	0.52	-0.85	9.895±0.053	0.54	-1.05

类型	0.60 μg/g		2.00 μg/g		4.00 μg/g
类型	平均回收率/%	RSD/%	平均回收率/%	RSD/%	平均回收率/%	RSD/%
唇膏	95.8	7.6	99.2	4.6	99.4	5.7
唇蜜	103.8	5.2	95.5	6.8	95.3	3.9
唇彩	96.9	5.3	98.7	8.9	100.2	4.2
唇釉	94.3	5.9	96.8	3.1	98.8	7.2

高效液相色谱法测定唇用化妆品中对位红含量

Determination of para red in lip cosmetics by high performance liquid chromatography

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