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日用化学工业（中英文）

日用化学工业（中英文） ›› 2026, Vol. 56 ›› Issue (10): 1344-1353.doi: 10.3969/j.issn.2097-2806.2026.10.007

• 基础研究 • 上一篇    下一篇

益生菌发酵产物通过Notch信号通路修护皮肤屏障的斑马鱼模型评价及机制研究

李鑫1,3,段治2,张芷茵3,崔洪昌2,4,*()   

  1. 1 北京工商大学 轻工科学与工程学院 化妆品系， 北京 100048
    2 青岛蔚蓝生物集团有限公司， 山东 青岛 266000
    3 诺斯贝尔化妆品股份有限公司， 广东 中山 528427
    4 中国海洋大学 食品科学与工程学院， 山东 青岛 266000
  • 收稿日期:2025-12-22 修回日期:2026-09-11 出版日期:2026-10-22 发布日期:2026-10-30

Evaluation of skin barrier repair by a probiotic ferment lysate in a zebrafish model and mechanistic exploration via the Notch signaling pathway

Xin Li1,3,Zhi Duan2,Zhiyin Zhang3,Hongchang Cui2,4,*()   

  1. 1 Department of Cosmetics, School of Light Industry Science and Engineering, Beijing Technology and Business University, Beijing 100048, China
    2 Qingdao Vland Biotech Co., Ltd., Qingdao, Shandong 266000, China
    3 Nox Bellcow Cosmetics Co., Ltd., Zhongshan, Guangdong 528427, China
    4 College of Food Science and Engineering, Ocean University of China, Qingdao, Shandong 266000, China
  • Received:2025-12-22 Revised:2026-09-11 Online:2026-10-22 Published:2026-10-30
  • Contact: E-mail: cuihc@vlandgroup.com

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摘要：

文章研究目的是评估护肤原料乳酸杆菌发酵溶胞产物VHProbi MixR的皮肤屏障修护功效，并阐明其潜在作用机制。该原料由四种益生菌发酵产物裂解液等比例混合制成。采用LC-MS非靶向代谢组学分析VHProbi MixR的化学成分，通过网络药理学预测其作用靶点及相关通路，构建乳酸诱导的斑马鱼皮肤屏障损伤模型，评价不同浓度原料的修护效果，结合转录组学和RT-qPCR验证核心通路及差异基因表达。结果表明VHProbi MixR含2 176种代谢物，主要包括有机酸及其衍生物、脂质及其类似物等，含烟酰胺等多种活性成分。网络药理学筛选出678个潜在靶点，关键靶点GSK3B与皮肤屏障修复相关通路密切相关。斑马鱼实验显示，VHProbi MixR （50和200 mL/L） 可显著降低受损皮肤的荧光染色面积（P<0.001），且高剂量组效果更优。转录组学及RT-qPCR验证表明，该原料可激活Notch信号通路，上调her4、her12、hey1等下游靶基因表达。研究结论为VHProbi MixR通过作用于GSK3B靶点激活Notch信号通路，促进角质形成细胞分化及紧密连接蛋白调控，从而发挥皮肤屏障修护作用，具有良好的护肤应用潜力。

关键词: VHProbi MixR, 皮肤屏障, Notch信号通路, GSK3B, 修护

Abstract:

This study aims to evaluate the skin barrier repair efficacy of the skincare ingredient Lactobacillus fermentation lysate VHProbi MixR and clarify its potential mechanism of action. This material is an equal-ratio mixture of lysates derived from four probiotic ferments. LC-MS-based untargeted metabolomics was used to analyze the chemical components of VHProbi MixR. Network pharmacology was employed to predict its potential targets and related pathways. A lactic acid-induced zebrafish skin barrier injury model was established to assess the repair effect of VHProbi MixR at different concentrations. Transcriptomics and RT-qPCR were combined to verify the core pathways and differential gene expression. VHProbi MixR contains 2 176 metabolites, mainly including organic acids and their derivatives, lipids and lipid-like molecules, with various active components such as nicotinamide. Network pharmacology identifie 678 potential targets, among which the key target GSK3B is closely associated with skin barrier repair-related pathways. Zebrafish experiments show that VHProbi MixR （50 and 200 mL/L） significantly reduces the fluorescent staining area of damaged skin （P<0.001）, with a more pronounced effect in the high-dose group. Transcriptomic and qPCR verification indicate that VHProbi MixR activates the Notch signaling pathway and upregulates the expression of downstream target genes, including her4, her12, and hey1. Collectively, VHProbi MixR exerts skin barrier repair effects by acting on the GSK3B target to activate the Notch signaling pathway, thereby promoting keratinocyte differentiation and regulating tight junction proteins. These findings demonstrate its promising potential for application in skincare products.

Key words: VHProbi MixR, skin barrier, Notch signaling pathway, GSK3B, repair

中图分类号: 

  • TQ658