东阿阿胶对小鼠皮肤屏障损伤的修复作用研究

doi:10.3969/j.issn.2097-2806.2026.02.012

摘要/Abstract

摘要：

为研究东阿阿胶对小鼠皮肤屏障损伤的修复作用。采用胶带剥离法构建小鼠皮肤屏障损伤模型，并使用阿胶对小鼠进行灌胃给药。造模后，每天检测小鼠受损皮肤处的经皮失水量（Transepidermal water loss，TEWL）。并于造模第4天和第7天后，通过光学活体成像观察皮肤屏障损伤的修复效果。通过RT-qPCT检测丝聚蛋白（Filaggrin，FLG）、内披蛋白（Involucrin，IVL）、紧密连接蛋白闭锁小带蛋白1（Zonula occludens-1，ZO-1）与炎症因子白介素-1α（Interleukin-1α，IL-1α）、白介素-1β（Interleukin-1β，IL-1β）等的mRNA水平。通过免疫组化检测皮肤屏障相关蛋白的蛋白含量，免疫荧光检测胸腺基质淋巴生成素（Thymic stromal lymphopoietin，TSLP）的表达。结果显示，东阿阿胶显著降低胶带剥离小鼠的经皮失水量，降低皮肤厚度并改善表皮结构紊乱，上调FLG，IVL和ZO-1的mRNA相对表达量与蛋白表达，抑制TSLP的蛋白表达与炎症因子的mRNA相对表达量。结果表明，东阿阿胶通过调控屏障蛋白与炎症因子的表达实现皮肤屏障修复，为其在皮肤健康领域的应用提供理论依据。

关键词: 皮肤屏障损伤, 东阿阿胶, 胶带剥离, 丝聚蛋白

Abstract:

The effect of Dong’e Ejiao on repairing mechanical skin barrier injury in mice was investigated. A mouse skin barrier injury model was established using the tape-stripping method. Transepidermal water loss（TEWL）at the damaged skin site was measured after model establishment. The repair effect of donkey-hide gelatin on skin barrier damage was observed through the C-Cube multi-functional skin imaging instrument. Optical coherence tomography（OCT）imaging and HE staining were used to observe the effect of donkey-hide gelatin on the thickness of injured skin. The mRNA levels of filaggrin（FLG）, involucrin（IVL）, tight junction protein zonula occludens-1（ZO-1）, and inflammatory factors interleukin-1α（IL-1α）and interleukin-1β（IL-1β）were detected by RT-qPCR. The protein levels of skin barrier-related proteins were examined by immunohistochemistry（IHC）, and its regulatory effect on thymic stromal lymphopoietin（TSLP）was verified by immunofluorescence（IF）. The results show that Dong’e Ejiao significantly reduces TEWL in tape-stripped mice, decreases skin thickness, improves disordered epidermal structure, up-regulates the expression of FLG, IVL, and ZO-1, and suppresses the expression of TSLP and down-regulates the mRNA levels of inflammatory factors including IL-1α and IL-1β. These results indicate that Dong’e Ejiao repairs the skin barrier by modulating the expression of barrier proteins and inflammatory factors. This demonstrates its potential for application in the field of skin health and provides a theoretical basis for such use.

Key words: skin barrier damage, Dong’e Ejiao, tape stripping, filaggrin

中图分类号:

TQ658

高欣雨, 孙娅楠, 李俐霜, 金玉翠, 马淑骅, 王毅. 东阿阿胶对小鼠皮肤屏障损伤的修复作用研究[J]. 日用化学工业（中英文）, 2026, 56(2): 236-244.

Xinyu Gao, Yanan Sun, Lishuang Li, Yucui Jin, Shuhua Ma, Yi Wang. Study on the repairing effect of Dong’ e Ejiao on skin barrier injury in mice[J]. China Surfactant Detergent & Cosmetics, 2026, 56(2): 236-244.

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