柞蚕丝素肽脂质体的制备及其抗光损伤作用研究

doi:10.3969/j.issn.2097-2806.2026.01.009

摘要/Abstract

摘要：

本研究通过逆向旋转蒸发法建立了高包封率柞蚕丝素肽脂质体的制备工艺，同时对其抗光损伤作用进行评估。结果表明，优化单因素试验确定胆磷比1∶11，水相体积3 mL，PBS溶液pH 8.0，一次超声3 min，二次超声5 min，乳化温度40 ℃的制备工艺参数，获得较佳包封效果和合适粒径大小的柞蚕丝素肽脂质体。柞蚕丝素肽脂质体能够通过修复胶原、增强抗氧化、抗炎等作用改善小鼠胚胎成纤维细胞的光损伤；通过清除ROS水平、尾鳍修复来缓解斑马鱼的紫外损伤，证实了柞蚕丝素肽脂质体在抵御皮肤光损伤方面的潜力，适于作为护肤产品的应用开发。

关键词: 柞蚕丝素肽, 脂质体, 抗氧化, 抗炎, 光损伤修复

Abstract:

A preparation process of tussah silk fibroin peptide liposomes with high encapsulation efficiency and suitable particle size was established by the reverse rotary evaporation method, and their anti-photodamage effect was evaluated in this study. The results show that the optimal encapsulation effect of tussah silk fibroin peptide liposomes is achieved by optimizing the single factor test to ascertain the preparation parameters of the mass ratio of cholesterol to lecithin of 1∶11, the aqueous phase volume of 3 mL, the pH of PBS solution of 8.0, the first ultrasound of 3 min, the second ultrasound of 5 min, and the emulsification temperature of 40 ℃. The tussah silk fibroin peptide liposomes are capable of ameliorating the photodamage to mouse embryonic fibroblasts by repairing collagen, enhancing anti-oxidation and exerting anti-inflammatory effects. Ultraviolet damage of zebrafish is alleviated by reducing reactive oxygen species （ROS） levels and repairing the tail fin. This study confirms the potential of tussah silk fibroin peptide liposomes in resisting skin photodamage, thereby rendering them suitable for application in skin care products.

Key words: tussah silk fibroin peptide, liposome, antioxidant, anti-inflammatory, photodamage repair

中图分类号:

TQ658

米锐, 李学军, 王林美, 陈星合, 贾心悦, 都兴范. 柞蚕丝素肽脂质体的制备及其抗光损伤作用研究[J]. 日用化学工业（中英文）, 2026, 56(1): 73-81.

Rui Mi, Xuejun Li, Linmei Wang, Xinghe Chen, Xinyue Jia, Xingfan Du. Study on the preparation of tussah silk fibroin peptide liposomes and their anti-photodamage effects[J]. China Surfactant Detergent & Cosmetics, 2026, 56(1): 73-81.

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