欢迎访问《日用化学工业（中英文）》，今天是
日用化学工业（中英文）

日用化学工业 ›› 2022, Vol. 52 ›› Issue (1): 98-102.doi: 10.3969/j.issn.1001-1803.2022.01.015

• 分析与检测 • 上一篇    下一篇

高效液相色谱法测定化妆品中依克多因的含量

唐毓萍(),王艳旗,李慧良   

  1. 华熙生物科技股份有限公司 上海研发中心,上海 200131
  • 收稿日期:2021-03-17 修回日期:2021-12-28 出版日期:2022-01-22 发布日期:2022-01-19
  • 通讯作者: 唐毓萍

Determination of the content of ectoine in cosmetics by high performance liquid chromatography

Tang Yuping(),Wang Yanqi,Li Huiliang   

  1. Shanghai R&D Center, Bloomage BioTechnology Corporation Limited, Shanghai 200131, China
  • Received:2021-03-17 Revised:2021-12-28 Online:2022-01-22 Published:2022-01-19
  • Contact: Yuping Tang

RichHTML

40

PDF (PC)

605

赞

摘要：

建立高效液相色谱法测定化妆品中依克多因的分析方法,采用Agilent Poroshell 120 EC-C18色谱柱（100 mm×3.0 mm,2.7 μm）分离,以甲醇和pH为3.0的40 mmol/L磷酸二氢钠-10 mmol/L 1-庚烷磺酸钠缓冲溶液梯度洗脱,流速0.8 mL/min,柱温30 ℃,检测波长210 nm。采用外标法定量测定化妆品中的依克多因含量。结果表明,依克多因在5~800 mg/L的质量浓度范围内呈现良好线性关系,相关系数为0.999 8,方法的检出限和定量限分别为0.3和1.0 mg/L。该方法具有分离效率高、分析时间短、节省溶剂等优点,解决了依克多因在C18色谱柱上保留弱的问题。

关键词: 高效液相色谱法, 依克多因, 化妆品

Abstract:

A method for the determination of ectoine in cosmetics by high performance liquid chromatography （HPLC） was established. Agilent Poroshell 120 EC-C18 column （100 mm×3.0 mm, 2.7 μm）was used for sample analysis. Methanol and 40 mmol/L sodium dihydrogen phosphate-10 mmol/L sodium 1-heptanesulfonate solution （adjusted pH to 3.0 by phoshporic acid） were used as mobile phases for gradient elution, with a flow rate of 0.8 mL/min. The temperature of the column was 30 ℃. Samples were quantitated by diode array detector at 210 nm with external standard method. Result shows that the standard working curve of ectoine has good linear relationship in the range of 5-800 mg/L. The limit of detection and quantitation is 0.3 and 1.0 mg/L, respectively. The method has the advantages of high separation efficiency, short analysis time, and solvent cost saving. It solves the problem of ectoine retaining on C18 chromatography column.

Key words: high performance liquid chromatography （HPLC）, ectoine, cosmetics

中图分类号: 

  • TQ658