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Table of Content

    22 August 2021, Volume 51 Issue 8 Previous Issue    Next Issue
    Basic research
    Development and application of lignin-based self-emulsification oil-displacing agent for Linpan heavy oil
    Wang Zaihua,Zhang Jun,Feng Jiaying,Liu Mudan,Liu Jianguo,Chen Tao
    2021, 51 (8):  697-704.  doi: 10.3969/j.issn.1001-1803.2021.08.001
    Abstract ( 386 )   HTML ( 92 )   PDF (1620KB) ( 243 )  

    With the decline of international crude oil price, the previous petroleum-based oil-displacing agent (NB-5034A) is difficult to be applied in oil field because of its high cost. To solve this problem, the low cost lignin-based self-emulsification oil-displacing agent was studied. First, according to the characteristics of Linpan heavy oil, the formula of lignin-based self-emulsification oil-displacing agent (NB-50PA) was optimized in the lab; then the effects of concentration, temperature, displacement velocity and mode, rock properties and oil viscosity on oil recovery were evaluated using simulation tests; the application conditions of NB-50PA for Linpan heavy oil were obtained. According to the field experiment, NB-50PA can increase the average recovery of Linpan heavy oil by 24.51%, and the cost is only 1/3 of the previous petroleum-based self-emulsification oil-displacing agent.

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    Synthesis and emulsification properties of hydrophobically modified chitosan
    Meng Xinyu,Liu Huan,Hu Xueyi,Xia Yongmei
    2021, 51 (8):  705-710.  doi: 10.3969/j.issn.1001-1803.2021.08.002
    Abstract ( 689 )   HTML ( 17 )   PDF (2397KB) ( 601 )  

    As a natural polysaccharide, chitosan (CS) has excellent biocompatibility, biodegradability and renewability. Chitosan has different molecular weight and deacetylation degree. However, its strong hydrophilicity limits its application in food, cosmetics and other fields. Therefore, hydrophobic modification of chitosan can decrease the strong hydrophilicity and expand its application as emulsifier. That is, hydrophobic groups are introduced into its hydrophilic skeleton. However, the safety of chemical modifications needs to be considered. Maillard reaction does not use any chemical cross-linking agent, and the reaction process is simple and the products are biocompatible. As one of the main hydrophobic proteins, casein can be used to improve the amphiphilicity of polysaccharides. After enzymatic hydrolysis, casein hydrophobic peptides (CHP) can provide peptides with more hydrophobic groups. In this study, Maillard reaction was used to modify chitosan with CHP. The optimized conditions of Maillard reaction were temperature of 60 ℃, relative humidity of 75%, reaction time of three days, and the mass ratio of CHP to CS of 1∶3. The grafting degree was calculated through the content of free amino groups. The grafting degree for this series obtained was from 3.10% to 15.08%. In addition, the browning index increased as Maillard reaction was going on. Infrared spectroscopy and fluorescence spectroscopy were used for structural analysis. The vibration absorption peak of protein side chain changed after the reaction. The typical Schiff base was synthesized after Maillard reaction. Amadori product was formed after cyclization and rearrangement. The CHP-modified chitosan can stabilize emulsion. An O/W emulsion (50% olive oil in water) stabilized with 1% CHP-modified chitosan was prepared. The emulsion stabilized with CS breaks at the 7th day. In contrast, the emulsion stabilized with CHP-modified chitosan has large Zeta potential which provides large electrostatic repulsion between droplets, inhibiting demulsification, flocculation or coalescence of the emulsion. The particle size and particle size distribution of the emulsion have little change during the storage period. The emulsion has excellent long-term storage stability in 28 days.

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    Lecture of science and technology
    Cosmetic emulsions and new technologies of emulsification (Ⅳ) Construction and application of oil-in-oil emulsions
    Ding Jingjing,Ni Xinjiong,Yang Cheng,Sun Yajuan
    2021, 51 (8):  711-718.  doi: 10.3969/j.issn.1001-1803.2021.08.003
    Abstract ( 1028 )   HTML ( 37 )   PDF (2167KB) ( 657 )  

    An emulsion is a mixture of two or more immiscible liquids in which a discontinuous phase is dispersed into a continuous phase. A typical emulsion is oil-water system, which has many significant applications in various industries such as food, pharmaceutical, tissue engineering and cosmetic industries. In the past decades, oil-in-oil emulsions have attracted great interest due to their special non-aqueous property, which complements traditional oil-water emulsion systems, and realize the usage of chemicals that are incompatible with water. In addition, similar to oil-water systems, oil-in-oil emulsions can also be stabilized by block copolymers and solid particles. In this review, the construction and formation mechanism of oil-in-oil emulsions were introduced. Then, the recent advances in stabilizing strategies of oil-in-oil emulsions were summarized, and the applications of oil-in-oil emulsions in biomedicine, polymerization and cosmetics were discussed. Finally, the development direction of non-aqueous emulsions was prospected. This review provides a reference for the preparation and application of oil-in-oil emulsions.

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    Development and application
    Preparation of fatty alcohol ether sulfates with different sulfation degree
    Li Jianbo,Li Jiyun,Li Ping,Yang Xiaoyi,Guo Chaohua,Ren Xiaodan
    2021, 51 (8):  719-724.  doi: 10.3969/j.issn.1001-1803.2021.08.004
    Abstract ( 337 )   HTML ( 6 )   PDF (1464KB) ( 366 )  

    The products of fatty alcohol ether sulfates (AES) with different sulfation degree were obtained by sulfation of fatty alcohol ethers (AEO) with sulfur trioxide in a falling film sulfonator. The components in the products and their changes with sulfation degree were determined by measuring the content of anionic active substance, AEO and inorganic acid, combined with the hydroxyl value of AEO and gas quality analysis. The content of 1,4-dioxane in the products with different sulfation degree was analyzed by headspace gas chromatography. The results show that the AEO with low addition number of oxyethylene group has the priority of sulfation. Dodecanol and dodecyl polyoxyethylene ether react with sulfur trioxide more easily than tetradecanol alcohol and tetradecyl polyoxyethylene ether. The average addition number of polyoxyethylene groups in AEO can be adjusted by controlling the different sulfation degree and thus the distribution of oxyethylene groups in AEO can also be further regulated and narrowed. The content of 1,4-dioxane increases with the increase of sulfation degree.

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    Adsorption behaviors of didecyl quaternary ammonium salts with different counterions on polytetrafluoroethylene surface
    Zhi Lifei,Shi Xiufang,Zhang Erzhuang,Li Xiaoming,Wang Hui,Pan Ruili
    2021, 51 (8):  725-733.  doi: 10.3969/j.issn.1001-1803.2021.08.005
    Abstract ( 271 )   HTML ( 5 )   PDF (2062KB) ( 248 )  

    The sessile drop method was used to study the adsorption behaviors of a series of didecyl quaternary ammonium salts with different counterions and a Gemini quaternary ammonium salt on the surface of superhydrophobic material polytetrafluoroethylene (PTFE). The effects of the surface activity, type and concentration of surfactants on the contact angle were investigated, and the changes of spreading coefficient, adhesion tension and adhesion work were discussed. The results show that the contact angle of didecyl quaternary ammonium salts on the surface of PTFE shows a trend of first decreasing and then increasing with the increase of size of counterions. Among them, didecyldimethylammonium valerate (DDAV) which has the largest counterion shows the best wetting on the surface of PTFE; the spreading coefficient (S) is decreased first and then increased with the increase of counterion size; when the concentration of the surfactant is lower than cmc, there is a good linear relationship between adhesion and surface tension; when the concentration is larger than the cmc, the solution of the surfactant with small counterion forms a saturated adsorption layer at the solid-liquid interface and the gas-liquid interface. Surfactants with larger counterions reach saturation adsorption at the gas-liquid interface, but do not reach saturation at the solid-liquid interface. Their adsorption continues and the wetting performance is enhanced with the increase of concentration. Therefore, the influences of a series of didecyl quaternary ammonium salts on the surface of PTFE were obtained, and the adsorption model was established, which provided a theoretical basis for further understanding of the adsorption of such surfactants on the surface of hydrophobic materials, and facilitated the application of such surfactants.

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    Efficacy evaluation of carbonated skin care products
    Bai Xin,Chen Yutong,Shang Yazhuo
    2021, 51 (8):  734-740.  doi: 10.3969/j.issn.1001-1803.2021.08.006
    Abstract ( 1206 )   HTML ( 24 )   PDF (2669KB) ( 525 )  

    Carbon dioxide is a kind of gas that can be produced by the human body. It is non-toxic and harmless, and plays an important role in the physiological function of the human body. Carbon dioxide has been widely used to improve physical health in recent years. Carbonated skin care products are a kind of skin care products containing carbon dioxide or producing carbon dioxide during the process of use, and they have become one of the hot products in the skin care industry. Carbon dioxide present in the products or produced in use of the products can enter the skin and cause the Bohr effect and thus achieve the purpose of skin care. In the market of skin care products, the safety and efficacy of carbonated skin care products are gradually recognized and accepted by domestic consumers. However, most consumers know little about their working mechanism. In this paper, a commercially available carbonated skin care product was studied to explore the conditioning effects on women’s skin. The VISIA-CR skin detector and the Moisture Meter SC were used to study the effects of the carbonated skin care product on blood circulation, pores and absorption. The deep cleansing effect of carbonated skin care products was also discussed. The results show that carbonated skin care products can improve the local skin microcirculation and promote pore expansion, which provides conditions for the subsequent absorption of effective ingredients in the skin care products. Moreover, the pore expansion caused by carbonated skin care products is conducive to removing blackhead and deep skin cleansing. However, the improvement of local blood circulation and the pore expansion are temporary, that is, blood circulation will gradually weaken with time and finally return to normal level, and the pore size will gradually recover and even pore contraction phenomenon appears. Carbonated skin care products are expected to fundamentally solve basic skin problems by accelerating skin blood circulation, promoting the absorption of effective ingredients and effectively removing deep waste in the skin.

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    Process optimization and anti-inflammatory activity of the curcumin emulsion
    Liu Guanting,Hu Liuyun,Ma Shijing,Han Ping,Lin Li,Du Zhiyun
    2021, 51 (8):  741-747.  doi: 10.3969/j.issn.1001-1803.2021.08.007
    Abstract ( 459 )   HTML ( 19 )   PDF (1548KB) ( 335 )  

    An emulsion containing curcumin was obtained by high pressure homogenization. According to the particle size of the emulsion, the technological parameters for preparation were optimized. The stability of the curcumin emulsion under light and at high temperature was determined. Further, the curcumin emulsion was used to control the anti-inflammatory activities in lipopolysaccharide LPS induced cell model in vitro and TPA induced animal model, as well as its regulating effect on inflammatory factors. The results show that the optimized condition of high pressure homogenization for preparing the curcumin emulsion is being homogenized at 60 MPa for 1 h at 35 ℃, and repeated for 6 cycles. The particle size of the curcumin emulsion thus prepared is (200±50) nm and the curcumin content is 5%. The curcumin emulsion and the cosmetic formulas thereof have good stability under light and at 60 ℃. The curcumin emulsion has a significant inhibitory effect on NO induced by LPS when the concentration of the curcumin emulsion is 1-50 μg/mL, and the inhibition rate of the curcumin emulsion with 0.4 mg/ear against TPA induced ear edema in mice is larger than 57%. The mechanism may be through inhibiting the secretion of key cytokines IL-6 and TNF-α in the inflammatory reaction, thus exerting anti-inflammatory effect.

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    Research on the establishment of lipstick persistence evaluation system
    Cen Xiaojuan,Wang Ying,Zhou Heyi,Liu Weiyi,Zhou Lin,Pan Yao
    2021, 51 (8):  748-753.  doi: 10.3969/j.issn.1001-1803.2021.08.008
    Abstract ( 408 )   HTML ( 21 )   PDF (1175KB) ( 467 )  

    As a color cosmetic product, lipstick not only brings beauty, but also pursues the instant effect of makeup. Currently, there are few papers on lipstick makeup retention test. The aim of the study was to establish a comprehensive and objective evaluation index and grading map of lipstick durability. Firstly, the volunteers who applied lipstick were subjected to artificial interference processing to simulate the lipstick take-off process and the lip image of the volunteers were captured by VISIA-CR. Then Image Pro Plus (IPP) software was used to analyze the front face photos taken by VISIA-CR under natural light, and SPSS 22.0 statistical software was applied to perform correlation analysis and factor analysis between indicators. Finally a mathematical model was established to objectively determine the lipstick color persistence and to establish a grading map. The results show that in the objective evaluation of lipstick image data, the color and uniformity of the lipstick are the main factors influencing the calculation of makeup retention index. The established lipstick retention grading map divides the durability of lipstick into 6 levels. The retention rate of level 1, level 3 and level 6 are 100%, 89% and 15%, respectively. And the makeup retention rate decreases as the level increases. The research results can provide technical support for the determination of lipstick makeup retention. It can also be used for market development and consumer market research. The atlas gives an objective and quantitative judgment on lipstick rentention ability. The subjective judgment of the level of lipstick makeup removal will be affected by the surrounding environment and personal subjective consciousness. The model can be applied to judge the level of lipstick makeup removal by collecting relevant index data. In the future, it is interesting to explore other color lipstick models, such as orange, brown, pink, etc. This experimental method has laid a solid foundation for the experimental research and data analysis of lipstick.

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    Study on the effects of donkey milk in delaying skin aging
    Liu Chunyuan,Fan Yumei,He Jie,Zhou Guangyun,Su Ning,Liao Feng
    2021, 51 (8):  754-760.  doi: 10.3969/j.issn.1001-1803.2021.08.009
    Abstract ( 351 )   HTML ( 12 )   PDF (2866KB) ( 332 )  

    The anti-aging effect of donkey milk on skin was evaluated. More and more attention has been paid to donkey milk in recent years since its nutrition composition shows higher similarity to human milk than others. Donkey milk has not only high nutritional value but also a wide range of medicinal value. Herein, the measurement of cell activity, cell proliferation, extracellular matrix (collagen, matrix metalloproteinase) content and invitro wound healing by the scratch assay were used. Donkey milk was found to be safe and nontoxic up to the concentration of 0.63- 10 mg/mL. Bromodeoxyuridine (BrdU) assay data showed the proliferation of HDFn cells and HaCaT cells after 48 h, and it was found that when the concentration of donkey milk was 5 and 10 mg/mL, the proliferation of cells was increased significantly (p<0.01). The collagen in extracellular matrix was increased by 38% at 10 mg/mL donkey milk compared to the control group (p<0.01). The content of MMP-1 secreted by HDFn cells induced by UVB was significantly reduced after treated with 5 and 10 mg/mL donkey milk. In addition, wound healing assay exhibited significant wound closure and cell migration compared to the control group after HDFn cells being treated with donkey milk for 24 h. In conclusion, donkey milk can promote cell proliferation, increase collagen content, inhibit MMP-1 secretion, promote damage repair, and thereby delay skin aging. It provides a necessary scientific basis for the application of donkey milk in functional cosmetics; however, the roles and specific molecular mechanism in delaying skin aging need to be further explored.

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    Research on the anti-aging effect and safety of fermented Lycium ruthenicum broth
    Zhao Dan,Wang Changtao,Li Meng,Zhang Jiachan,Wang Dongdong
    2021, 51 (8):  761-767.  doi: 10.3969/j.issn.1001-1803.2021.08.010
    Abstract ( 752 )   HTML ( 19 )   PDF (1621KB) ( 384 )  

    Saccharomyces cerevisiae was used to ferment Lycium ruthenicum to obtain fermented Lycium ruthenicum broth (FLRB). The components of FLRB were detected and the safety and the anti-aging effect were evaluated. These results would provide theoretical basis for its application in the field of cosmetics. Firstly, the free radical scavenging ability of FLRB was tested at the biochemical level, and then the anti-aging effects were evaluated at cellular level, including collagen formation, expression of collagenase, and activities of antioxidant enzymes in human fibroblasts. Finally, the eye irritation and skin safety of the FLRB were tested by chick embryo chorioallantoic membrane and human patch experiments. Results show that the FLRB is rich in polysaccharides and proteins, and is less in flavonoids, polyphenols and proanthocyanidins. The FLRB has significant ability to scavenge DPPH free radicals and ABTS+free radicals, with IC50 values of 8.15% and 1.08%, respectively. The FLRB of 5% volume fraction can promote collagen production to a content being 137.84% (p<0.01) of the control group and can inhibit collagenase production to a content being 70.41% (p<0.01) of the control group. The activities of GSH-Px and CAT and the total antioxidant capacity are significantly (p<0.01) improved and reactive oxygen species is significantly (p<0.01) inhibited by the FLRB of 1% volume fraction. Chick embryo chorioallantoic membrane experiment and human patch test show that the FLRB is safe to human eyes and skin.

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    Study on risk assessment and use regulation of nitromethane in cosmetics
    Qin Meirong,Xian Jingwen,Wu Xi,Zhang Gaofei,Wu Xiaoou,Wang Ping,Wang Xiaowei
    2021, 51 (8):  768-774.  doi: 10.3969/j.issn.1001-1803.2021.08.011
    Abstract ( 369 )   HTML ( 14 )   PDF (1033KB) ( 439 )  

    Nitromethane has been classified by the International Agency for Research on Cancer (IARC, 2000) as a Group 2B carcinogen. There is sufficient evidence in experimental animals for the carcinogenicity of nitromethane. Relevant institutions and organizations have evaluated its safety and restricted its use all over the world. In the European Union and Malaysia, nitromethane is allowed in cosmetic products at a maximum concentration of 0.3%, and South Korea bans the use of nitromethane in cosmetics. In addition, China, United States and Japan all regulate the residue limit of nitromethane in drugs. Our key laboratory assessed the safety of its use in cosmetics, and aimed to provide advice and suggestion for cosmetics supervision. Via dermal absorption and inhalation are the main routes of exposure to nitromethane from products. Based on the current toxicological research data, we conducted the risk assessment of nitromethane in various cosmetic products such as mascara, eyeliner, body lotion and hair spray, and made comparative analysis of use regulations in various countries. The results show that the MoE value of nitromethane is less than 1 when the limit of it in cosmetics is 0.3%(w/w), so the carcinogenic risk of nitromethane should be concerned. Due to no cosmetics sold in China have been identified containing nitromethane at present, and individual cosmetic products containing nitromethane overseas are not added as cosmetic raw materials. It is recommended to include nitromethane in the list of prohibited substances in cosmetics.

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    Reviews
    Research progress on antibacterial activity of bioactive flavonoids
    Liang Haiyun,Ma Zemeng,Sun Ruiliang,Song Liya
    2021, 51 (8):  775-781.  doi: 10.3969/j.issn.1001-1803.2021.08.012
    Abstract ( 594 )   HTML ( 29 )   PDF (1055KB) ( 426 )  

    Flavonoids are widely distributed in nature, from fruits and vegetables to Chinese materia medica, Most of them have been found to have antibacterial activity, so they are the hot spot in the development of safe and natural preservatives. Plant derived flavonoids exist mostly in the form of glycosides and a few are in the form of free aglycones. As a kind of complex components, the antibacterial properties of plant flavonoids are closely related to the structure, classification and extraction methods of flavonoids. In this review, classification and antibacterial activity of bioactive flavonoids in plants was firstly summarized. Then the antibacterial structure-activity relationship trend of bioactive flavonoids was found by comparing the influence of different structure on the antimicrobial activity of flavonoids. Moreover, the antibacterial mechanisms of flavonoids were elucidated from the whole morphology, normal energy metabolism and inhibition of macromolecular synthesis. Finally, the application of bioactive flavonoids in plants as potential preservative in food, cosmetic and medicine were generalized. The main purpose of this review is to provide theoretical reference for flavonoids as antimicrobial agents.

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    Current situation and prospect of integrated strategies for skin sensitization testing
    Sun Fanghui,Song Xiaojie,Huo Gang
    2021, 51 (8):  782-788.  doi: 10.3969/j.issn.1001-1803.2021.08.013
    Abstract ( 422 )   HTML ( 20 )   PDF (1208KB) ( 326 )  

    In recent years, the incidence of cosmetic skin diseases such as cosmetic contact dermatitis and cosmetic acne has been increasing significantly, and the increasingly severe safety issues of cosmetics have aroused widespread concern in the society. Therefore, the state has promulgated a series of laws and regulations to further regulate the safety of cosmetics, and the enterprises must also carry out sufficient safety evaluation and risk substance evaluation on cosmetics raw materials and finished products. Skin sensitization is one of the toxicological end points of cosmetic safety evaluation. Although traditional animal experiments can meet the requirements of skin sensitization testing, they do not comply with the 3R principle. The European Union has imposed a total ban on animal testing of cosmetic ingredients and on the sale of cosmetic ingredients and finished products tested on animals. Therefore, alternative methods have gradually replaced animal experiments as an important tool for cosmetic safety evaluation. At present, the alternative methods that have certified by OECD mainly include direct peptide reaction test, KeratinoSensTM test, human cell line activation test and so on. However, a single substitution test is usually insufficient to accurately determine the skin sensitization of a compound. Therefore, different single experimental results are often combined according to the AOP pathway into an integrated strategy to comprehensively determine the skin sensitization of compounds, so as to improve the prediction accuracy. At present, the existing integrated strategies are mainly developed by BASF, L ’Oreal, Shiseido and other industries, including the ‘2 out of 3’ principle, stacking Meta model, ANN-EC3 model, etc. Due to the late introduction of animal alternative methods in China, domestic cosmetic companies and institutions have not yet developed their own integrated models, but we are working hard to develop new skin sensitization alternative technologies, such as the construction of real-time gene expression reporter cells, the development of new QSAR computer system and the construction of THP-1/KC co-culture system. Good results have been achieved in these new technologies. This paper summarizes and expounds the integrated strategies and methods of skin sensitization test currently used by daily chemical related enterprises, and summarizes the development status of self-developed skin sensitization alternative methods in China.

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    Analytical methods and testing
    Rapid determination of antimicrobial agents in hair care cosmetics by high performance liquid chromatography-tandem mass spectrometry
    He Xiaowei
    2021, 51 (8):  789-794.  doi: 10.3969/j.issn.1001-1803.2021.08.014
    Abstract ( 246 )   HTML ( 13 )   PDF (1188KB) ( 247 )  

    In order to determine the hormone- and amine-type forbidden antibacterial drugs in hair-care cosmetics, a high performance liquid chromatography-tandem mass spectrometry-based method for was proposed. Taking Schwarzkopf professional repairing and brightening hair care cream as the research object, using methanol as the extraction solution, the antibacterial drugs in hair care cosmetics were determined by octadecylsilane-bonded silica gel chromatographic column in multi-reaction detection mode. The results show that when 0.02 mol/L ammonium formate aqueous solution is used as the flow phase, the superimposed total ion current separation effect of hormone-type forbidden antibacterial drugs in hair care cosmetics is the best. The isotope abundance ratio is the highest. When the extraction solvent is methanol, the recovery rates of A-1 to A-10 hormone-type and B-1, B-2, and B-3 amine-type forbidden antibacterial drugs are the highest, which are greater than 90% and 95%, respectively. For this reason, this article uses methanol as the sample extraction solution for the determination of 10 hormone-type forbidden antibacterial drugs. The results show that in Schwarzkopf Professional Repairing and Brightening Hair Care Cream, the detection limits of 10 hormone-type forbidden antibacterial drugs and 3 amine-type forbidden antibacterial drugs are all lower than the quantitative limit. The recovery and precision of hormone-type forbidden antibacterial drugs are 84.7%-114.7% and 1.8%-9.8%, respectively. The recovery and precision of amine-type forbidden antibacterial drugs are 84.1%-93.9% and 1.4%-5.7%, respectively. The separation time is less than 2 min and the determination time is less than 4 min. In Schwarzkopf Professional Repairing and Brightening Hair Care Cream, there are no hormone-type forbidden antibacterial drugs and amine-type forbidden antibacterial drugs, and the measurement efficiency of antibacterial drugs can meet the measurement requirements.

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    Determination of 15 caine-type prohibited ingredients in cosmetics by solid phase extraction-high performance liquid chromatography
    Lu Li,Fang Fang,Feng Youlong,Cao Ling
    2021, 51 (8):  795-801.  doi: 10.3969/j.issn.1001-1803.2021.08.015
    Abstract ( 222 )   HTML ( 15 )   PDF (1200KB) ( 225 )  

    A solid phase extraction- high performance liquid chromatographic(HPLC) method was developed for the simultaneous determination of 15 caine-type prohibited ingredients in cosmetics. The samples were extracted by different solvent, then purified by solid phase extraction. Chromatographic separation was achieved on an C18 chromatographic column (250 mm×4.6 mm×5 μm). The column temperature was 30 ℃ through gradient elution with methanol-aqueous solution of 0.01 moL/L Na2HPO4 (pH 7.0) as binary mobile phase at a flow rate of 1.0 mL/min, and the detection wavelength was 230 nm. The standard curve method was used for quantification. The results show that the linear correlation coefficients of the 15 compounds are all greater than 0.999. The LODs of 15 caine-type prohibited ingredients are in the range of 1-13 mg/kg, while the LOQs are in the range of 3-29 mg/kg. The average recoveries at two spiked levels of astringent, milk and oil matrix are in the range of 90.9%-108.0%, 87.5%-104.5% and 87.7%-105.0% respectively, with RSDs of 0.1%-3.6%, 1.1%-8.8% and 0.5%-6.0% respectively. This method is proved to be efficient, accurate and durable, which provids an effective detection technique for 15 caine-type prohibited ingredients in cosmetics

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    Determination of 16 kinds of anti-infective drugs in cosmetics by LC-MS/MS
    Sha Lina,Yang Guangyong,Mu Xiaojuan,Zhang Mingyue
    2021, 51 (8):  802-808.  doi: 10.3969/j.issn.1001-1803.2021.08.016
    Abstract ( 362 )   HTML ( 15 )   PDF (1126KB) ( 419 )  

    A method based on liquid chromatography tandem mass spectrometry (LC-MS/MS) was established for the determination of 16 kinds of anti-infective drugs in cosmetics. The linear range of ketoconazole is 0.50-25 μg/g, and the linear range of the other 15 components is between 0.10-5.0 μg/g. The correlation coefficients are all greater than 0.99, and the RSD of precision test is less than 1.8%. The detection mass concentration of ketoconazole is 25 ng/mL, and the detection mass concentrations of the other 15 components are less than 10 ng/mL. The recoveries are in the range of 86.0%-112.4%. The method has good linearity, precision and recovery. It is suitable for the detection of 16 kinds of anti-infective drugs in cosmetics. Combined with the national standard method, this method adds the detection of 8 components, strengthens the supervision of cosmetics and reduces the safety risk of cosmetics.

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