黑枸杞发酵液的抗衰老功效与安全性探究

doi:10.3969/j.issn.1001-1803.2021.08.010

Abstract

Abstract:

Saccharomyces cerevisiae was used to ferment Lycium ruthenicum to obtain fermented Lycium ruthenicum broth （FLRB）. The components of FLRB were detected and the safety and the anti-aging effect were evaluated. These results would provide theoretical basis for its application in the field of cosmetics. Firstly, the free radical scavenging ability of FLRB was tested at the biochemical level, and then the anti-aging effects were evaluated at cellular level, including collagen formation, expression of collagenase, and activities of antioxidant enzymes in human fibroblasts. Finally, the eye irritation and skin safety of the FLRB were tested by chick embryo chorioallantoic membrane and human patch experiments. Results show that the FLRB is rich in polysaccharides and proteins, and is less in flavonoids, polyphenols and proanthocyanidins. The FLRB has significant ability to scavenge DPPH free radicals and ABTS⁺free radicals, with IC₅₀ values of 8.15% and 1.08%, respectively. The FLRB of 5% volume fraction can promote collagen production to a content being 137.84% （p<0.01） of the control group and can inhibit collagenase production to a content being 70.41% （p<0.01） of the control group. The activities of GSH-Px and CAT and the total antioxidant capacity are significantly （p<0.01） improved and reactive oxygen species is significantly （p<0.01） inhibited by the FLRB of 1% volume fraction. Chick embryo chorioallantoic membrane experiment and human patch test show that the FLRB is safe to human eyes and skin.

Key words: Lycium ruthenicum, fermentation, anti-aging, safety

CLC Number:

TQ658

Zhao Dan,Wang Changtao,Li Meng,Zhang Jiachan,Wang Dongdong. Research on the anti-aging effect and safety of fermented Lycium ruthenicum broth[J].China Surfactant Detergent & Cosmetics, 2021, 51(8): 761-767.

Figures/Tables 8

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References 20

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ρ/（g·L^-1）
总糖	多酚	黄酮	蛋白质	原花青素
26.302±1.356	0.064±0.001	0.016 6±0.000 7	1.734±0.110	0.145±0.028

样品名称	症状	去除斑试器不同时间后反应人数/个
样品名称	症状	30 min后	24 h后	48 h后
空白	无反应	30	30	30
空白	不良反应	0	0	0
黑枸杞发酵液	无反应	29	30	30
黑枸杞发酵液	不良反应	疑似1例	0	0

Research on the anti-aging effect and safety of fermented Lycium ruthenicum broth

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