姜黄素乳液的工艺优化及其抗炎活性研究

doi:10.3969/j.issn.1001-1803.2021.08.007

Abstract

Abstract:

An emulsion containing curcumin was obtained by high pressure homogenization. According to the particle size of the emulsion, the technological parameters for preparation were optimized. The stability of the curcumin emulsion under light and at high temperature was determined. Further, the curcumin emulsion was used to control the anti-inflammatory activities in lipopolysaccharide LPS induced cell model in vitro and TPA induced animal model, as well as its regulating effect on inflammatory factors. The results show that the optimized condition of high pressure homogenization for preparing the curcumin emulsion is being homogenized at 60 MPa for 1 h at 35 ℃, and repeated for 6 cycles. The particle size of the curcumin emulsion thus prepared is （200±50） nm and the curcumin content is 5%. The curcumin emulsion and the cosmetic formulas thereof have good stability under light and at 60 ℃. The curcumin emulsion has a significant inhibitory effect on NO induced by LPS when the concentration of the curcumin emulsion is 1-50 μg/mL, and the inhibition rate of the curcumin emulsion with 0.4 mg/ear against TPA induced ear edema in mice is larger than 57%. The mechanism may be through inhibiting the secretion of key cytokines IL-6 and TNF-α in the inflammatory reaction, thus exerting anti-inflammatory effect.

Key words: curcumin, emulsion, anti-inflammatory, stability

CLC Number:

TQ658

Liu Guanting,Hu Liuyun,Ma Shijing,Han Ping,Lin Li,Du Zhiyun. Process optimization and anti-inflammatory activity of the curcumin emulsion[J].China Surfactant Detergent & Cosmetics, 2021, 51(8): 741-747.

Figures/Tables 6

Fig. 1

Fig. 2

Fig. 3

Tab. 1

Tab. 2

Tab. 3

References 19

[1]	He Yan, Liu Hongfei, Bian Wangqing, et al. Molecular interactions for the curcumin-polymer complex with enhanced anti-inflammatory effects[J]. Pharmaceutics, 2019, 11(9) : 442. doi: 10.3390/pharmaceutics11090442
[2]	Tsuda Takanori. Curcumin as a functional food-derived factor: degradation products, metabolites, bioactivity, and future perspectives[J]. Food & Function, 2018, 9(2) : 705-714.
[3]	Ghosh Siddhartha S, He Hongliang, Wang Jing, et al. Curcumin-mediated regulation of intestinal barrier function: The mechanism underlying its beneficial effects[J]. Tissue Barriers, 2018, 6(1) : e1425085. doi: 10.1080/21688370.2018.1425085
[4]	He Yan, Yue Yuan, Zheng Xi, et al. Curcumin, inflammation, and chronic diseases: how are they linked?[J]. Molecules, 2015, 20(5) : 9183-9213. doi: 10.3390/molecules20059183 pmid: 26007179
[5]	Devassy Jessay G, Nwachukwu Ifeanyi D, Jones Peter J H. Curcumin and cancer: barriers to obtaining a health claim[J]. Nutrition Reviews, 2015, 73(3) : 155-165. doi: 10.1093/nutrit/nuu064 pmid: 26024538
[6]	Subash C Gupta, Gorkem Kismali, Bharat B Aggarwal. Curcumin, a component of turmeric: From farm to pharmacy[J]. BioFactors, 2013, 39(1) : 2-13. doi: 10.1002/biof.1079 pmid: 23339055
[7]	Tao Hui, Yu Chuqin, Huang Jingheng, et al. The solubility and stability of curcumin[J]. Food and Fermentation Industries, 2016, 42(8) : 160-165.
[8]	Ke Xiumei, Yang Rongping, Guo Xiangxiang, et al. Solubilization of self-emulsifying drug delivery system (SEDDS) for curcumin components[J]. Chinese Traditional and Herbal Drugs, 2014, 45(7) : 935-941.
[9]	Du Zhiyun, Xu Xuetao, Pan Wenlong, et al. Study of inhibition of curcuminoids and curcumin derivatives on tyrosinase[J]. China Surfactant Detergent& Cosmetics, 2008 (3) : 172-175.
[10]	Ye Feng, Xiao Weili, Wen Guojin, et al. Study on the cutaneous permeation of tetrahydrocurcumin in various formulations[J]. China Surfactant Detergent& Cosmetics, 2017, 47(10) : 573-577.
[11]	Zhang Yan, Duan Xueqin, Gao Gang, et al. Study on optimization of extraction process for curcuminoids by response surface method and its antioxidant activity[J]. Science and Technology of Food Industry, 2015, 36(6) : 269-273, 278.
[12]	Tang Lei, Wu Jian, Yu Guran, et al. Effect of Yiqi Yangyin Zhuyu recipe in anti-inflammation activities in vitro on LPS-induced RAW 264. 7 cells[J]. Chinese Journal of Experimental Traditional Medical Formulae, 2019, 25(1) : 141-148.
[13]	Cong Youquan, Han Jingjing, Fang Yun, et al. Protective effects of total flavones from Xueli Formula on LPS-stimulated RAW264.7 cell inflammation[J]. Chinese Traditional Patent Medicine, 2017, 39(5) : 890-895.
[14]	Cao Wen, Liu Huan, Han Qingjuan, et al. Anti-inflammation effect of (E)-phenethyl 3-(3, 5-dihydroxy-4-isopropylphenyl) acrylate and the preliminary mechanism[J]. Immunological Journal, 2015, 31(1) : 12-16.
[15]	Krog N J, Spars F V. Food emulsifiers: their structures and properties[J]. Food Emulsions, 2004, 4:44-90.
[16]	Kang Ji, Guo Qingbin, Glyn O Phillips, et al. Understanding the structure-emulsification relationship of gum ghatti-a review of recent advances[J]. Food Hydrocolloids, 2014, 42:187-195. doi: 10.1016/j.foodhyd.2014.04.010
[17]	Ping Shun, Wang Kai, Zhang Jianglin, et al. In vitro anti-flammatory effects of Camellia Sinensis Bee Pollen Extract(BPE) on LPS-induced inflammation in RAW 264.7 macrophage cells[J]. Journal of Food Science and Biotechnology, 2015, 34(12) : 1302-1307.
[18]	Shirley Boller, Cristian Sold, Maria C A Marques, et al. Anti-inflammatory effect of crude extract and isolated compounds from Baccharis illinita DC in acute skin inflammation[J]. Journal of Ethnopharmacology, 2010, 130(2) : 262-266. doi: 10.1016/j.jep.2010.05.001 pmid: 20452414
[19]	Masao Murakawa, Kumiko Yamaoka, Yoshitaka Tanaka, et al. Involvement of tumor necrosis factor (TNF)-α in phorbol ester 12-O-tetradecanoylphorbol-13-acetate (TPA)-induced skin edema in mice[J]. Biochemical Pharmacology, 2006, 71(9) : 1331-1336. pmid: 16487490

实验组	质量浓度/ （μg·mL^-1）	NO含量/ （μmol·L^-1）
对照组	—	28.55±0.62
模型组（LPS）	2	64.46±0.80^###
姜黄素	1	61.01±0.87
	5	56.97±0.54
	15	54.57±0.79^*
	25	49.08±1.17^**
	50	46.33±0.54^**
姜黄素乳液	1	51.12±0.70^*
	5	45.68±0.88^**
	15	41.18±0.56^**
	25	37.61±1.15^***
	50	33.35±0.88^***

组别	耳片重量差值/mg （m_左耳-m_右耳,$\bar{x}$±s）	抑制率/%
空白组	0.53±0.39
模型组	22.20±0.89^###
地塞米松（0.05 mg/ear）	3.27±0.42^***	85.29^***
姜黄素（0.2 mg/ear）	18.50±1.25	16.67
姜黄素（0.4 mg/ear）	13.76±0.86^*	38.02^*
姜黄素纳米乳液（0.2 mg/ear）	11.26±0.79^**	49.28^**
姜黄素纳米乳液（0.4 mg/ear）	9.38±0.95^***	57.75^***

组别	含量/（pg·mg^-1）
组别	TNF-α	IL-6
空白组	55.74±2.08	11.90±1.14
模型组	109.18±4.49^###	30.85±2.71^###
地塞米松（0.05 mg/ear）	60.79±1.92^***	15.50±0.56^***
姜黄素（0.2 mg/ear）	94.70±2.77	28.73±0.62
姜黄素（0.4 mg/ear）	89.53±1.74^*	25.36±1.58^*
姜黄素纳米乳液（0.2 mg/ear）	81.71±1.70^**	24.69±1.68^*
姜黄素纳米乳液（0.4 mg/ear）	69.99±2.30^***	16.95±0.47^***

Process optimization and anti-inflammatory activity of the curcumin emulsion

RichHTML

PDF (PC)

Like

Knowledge

Abstract

Cite this article

share this article

Figures/Tables 6

References 19

Related Articles 15

Metrics

Comments

Recommended 10

[1]	Kangfu Zhou, Yixuan Zhi, Feifei Wang, Yazhuo Shang. New emulsion system and its application in cosmetics （VI）Microemulsion [J]. China Surfactant Detergent & Cosmetics, 2024, 54(2): 139-148.
[2]	Weilan Liu, Li Li, Chunnian He, Yinmao Dong, Xiao Ling, Miaomiao Guo. Study on the inhibitory effect of total glycosides of Paeonia veitchii and its main component paeoniflorin on acne-associated inflammation [J]. China Surfactant Detergent & Cosmetics, 2024, 54(2): 188-195.
[3]	Xiaohong Pan, Ziqi Gao, Zhen Chen, Shuai Yin, Haiping Huang, Bin Hu. Discussion on the current situation of research and management on the stability of cosmetic products in China [J]. China Surfactant Detergent & Cosmetics, 2024, 54(2): 201-208.
[4]	Pei Liu, Ting Pan, Xiaomei Pei, Binglei Song, Jianzhong Jiang, Zhenggang Cui, Bernard P. Binks. Dual-responsive oil-in-water emulsions co-stabilized by a nonionic-anionic Bola surfactant and silica nanoparticles [J]. China Surfactant Detergent & Cosmetics, 2024, 54(1): 1-15.
[5]	Xu Han, Jiajia Wu, Na Wu, Yazhuo Shang. New emulsion system and its application in cosmetics （V） Janus emulsion [J]. China Surfactant Detergent & Cosmetics, 2024, 54(1): 24-31.
[6]	Yaru Wang, Tingyuan Mo, Hongxia Lai, Yue Zhou, Jiaying Xie, Jianhua Tan. Analysis of the causes of skin irritation of niacinamide cosmetics based on patch test and stability test [J]. China Surfactant Detergent & Cosmetics, 2024, 54(1): 51-56.
[7]	Xue Xiao, Huirong Zhang, Shuowen Li, Sunhua Li, Miaomiao Guo, Li Li. Determination of four polyphenols and study on antioxidant and anti-inflammatory activities of extracts from callus of Eryngium maritimum L. [J]. China Surfactant Detergent & Cosmetics, 2024, 54(1): 57-64.
[8]	Zhou Kangfu, Yan Jiayi, Shang Yazhuo. New emulsion system and its application in cosmetics （I）Liquid crystal emulsion system [J]. China Surfactant Detergent & Cosmetics, 2023, 53(9): 1008-1017.
[9]	Fang Tinghuan, Zheng Ting, Jiang Qing, Li Xiaoxia, Tang Lirong. Synergistic effect of silk peptides thermophilus fermentation on the skin anti-inflammatory and anti-aging activities [J]. China Surfactant Detergent & Cosmetics, 2023, 53(9): 1057-1064.
[10]	Wang Huazheng, Zhang Liang, Kang Xin, Kang Wanli, Li Zhe, Yang Hongbin. Effect of CO₂ on physical properties of produced oil and water in Changqing and emulsion stabilization mechanism [J]. China Surfactant Detergent & Cosmetics, 2023, 53(6): 617-624.
[11]	Gao Dangge, Li Suirong, Wang Lili, Lv Bin, Zhao Jing. Properties of a fracturing fluid thickener synthesized by inverse emulsion polymerization based on water/isoamyl octanoate-white oil [J]. China Surfactant Detergent & Cosmetics, 2023, 53(6): 625-633.
[12]	Zhen Enlong, Zhang Wen, Qian Zhen, Du Ruotong, Wang Yang. Construction and plugging performance evaluation of emulsified thermosetting resin system [J]. China Surfactant Detergent & Cosmetics, 2023, 53(6): 649-657.
[13]	Zhang Huirong, Guo Miaomiao, Chen Chen, Pan Qianyin, Zhang Ying, Li Li. The preparation process of three leguminous plant peptides and their activities [J]. China Surfactant Detergent & Cosmetics, 2023, 53(4): 423-429.
[14]	Ding Zhengqing, Wu Yingyi, Wang Weiyun, Huang Xujuan, Cai Zhaosheng. Study on Pickering emulsion stabilized by hydroxyethyl cellulose/nanocellulose and its rheological properties [J]. China Surfactant Detergent & Cosmetics, 2023, 53(3): 245-252.
[15]	Lv Yawen,Wang Jingwen,Ye Zhicheng,Shang Yazhuo,Liu Honglai. Preparation of P/O/LC/W multiple emulsion [J]. China Surfactant Detergent & Cosmetics, 2023, 53(2): 121-132.