侧柏叶提取物多元醇提取工艺研究及其对人真皮乳头细胞的影响

doi:10.3969/j.issn.2097-2806.2025.11.009

Abstract

Abstract:

Using an aqueous 1,4-butanediol solution as the solvent combined with ultrasonic-assisted extraction, total flavonoids from Platycladus orientalis leaves were extracted. The extraction process was optimized using response surface methodology with the yield of total flavonoids, quercitrin, and myricitrin as the evaluation indicators. The optimized extraction conditions are as follows: 40 wt% 1,4-butanediol, extraction temperature of 60 ℃, extraction time of 60 min, and solid-to-liquid ratio of 1∶10（g∶mL）. Under these optimized conditions, the yield of total flavonoids is 50.74 mg/g, while the yields of quercitrin and myricitrin are 3.65 mg/g and 2.10 mg/g, respectively. Compared with water and ethanol extraction, the stability of the extract is significantly improved. Furthermore, the efficacy of the 1,4-butanediol-extracted Platycladus orientalis leaf extract on human dermal papilla cells （HDPCs） was evaluated in comparison with the extracts obtained using traditional solvents （water and ethanol）. Cell viability, changes in vascular endothelial growth factor （VEGF） in the cell culture supernatant, as well as the inhibitory effects on intracellular reactive oxygen species （ROS） and the inflammatory factor IL-6 were measured. The results indicate that, compared with the extracts obtained using water or ethanol, the 1,4-butanediol extract more effectively promotes the proliferation of HDPCs and enhances VEGF secretion. It also demonstrates significantly stronger inhibition of ROS and IL-6, suggesting its potential efficacy in promoting hair growth, as well as in scalp antioxidant and anti-inflammatory applications.

Key words: Platycladus orientalis leaves, flavonoids, 1, 4-butanediol, human dermal papilla cells

CLC Number:

TQ658

Xianqi Chen, Timson Chen, Ling Ma, Ya Chen, Zhizhen Li, Guangli Wang, Jing Wang. Study on the polyol extraction process of Platycladus orientalis leaf extract and its effects on human dermal papilla cells[J].China Surfactant Detergent & Cosmetics, 2025, 55(11): 1435-1443.

Figures/Tables 11

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来源	平方和	自由度	均方	F值	P值	显著性
模型	506.98	9	56.33	13.05	0.001 3	**
A	55.06	1	55.06	12.76	0.009 1	**
B	33.29	1	33.29	7.71	0.027 4	*
C	33.59	1	33.59	7.78	0.026 9	*
AB	1.99	1	1.99	0.46	0.519 1	—
AC	0.29	1	0.29	0.067	0.803 7	—
BC	0.86	1	0.86	0.20	0.667 9	—
A²	55.32	1	55.32	12.82	0.009 0	**
B²	66.91	1	66.91	15.50	0.005 6	**
C²	224.66	1	224.66	52.05	0.000 2	**
残差	30.21	7	4.32
失拟值	8.51	3	2.84	0.52	0.689 3	—
纯误差	21.70	4	5.43
总和	537.19	16

Study on the polyol extraction process of Platycladus orientalis leaf extract and its effects on human dermal papilla cells

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水平	因素
水平	A 溶剂质量分数/%	B 提取温度/℃	C 提取时间/min
-1	20	50	30
0	40	60	60
1	60	70	90

序号	A 1,4-丁二醇质量分数/%	B 提取温度/^oC	C 提取时间/min	Y 总黄酮得率/（mg/g）
1	20	50	60	37.54
2	60	50	60	42.97
3	20	70	60	44.63
4	60	70	60	47.24
5	20	60	30	34.54
6	60	60	30	40.48
7	20	60	90	38.54
8	60	60	90	45.55
9	40	50	30	36.81
10	40	70	30	38.36
11	40	50	90	39.54
12	40	70	90	42.95
13	40	60	60	49.18
14	40	60	60	54.33
15	40	60	60	49.14
16	40	60	60	49.09
17	40	60	60	51.79

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