洋甘菊总黄酮提取及其抗氧化活性研究

doi:10.3969/j.issn.2097-2806.2024.09.004

Abstract

Abstract:

By optimizing the extraction and purification process, the study first used supercritical CO₂ to remove the lipophilic components of chamomile, and then combined with ultrasound assisted the extraction of total flavonoids from chamomile. The total flavonoids in chamomile were separated and purified using macroporous resin, and the total flavonoid components were analyzed and characterized. The optimal process conditions for obtaining supercritical CO₂ to remove fat soluble components from chamomile are: extraction temperature of 50 ℃, extraction time of 60 minutes, extraction pressure of 25 MPa, and chamomile fat removal rate of 8.66%. The optimal process conditions for ultrasound assisted extraction of total flavonoids from chamomile are: ultrasound time of 15 minutes, ethanol volume fraction of 50%, solid-liquid ratio of 1∶25 （g∶mL）, ultrasound power of 200 W, and total flavonoid extraction rate of 4.25%. After purification, the purity of total flavonoids in chamomile increase from 6.04% to 52.25%, while the purity of total flavonoids in chamomile is only 25.47% using the same purification process using a single ultrasound assisted extraction method without supercritical CO₂ extraction. The results show that the combination of supercritical CO₂ extraction and ultrasound assisted extraction can more efficiently extract total flavonoids from chamomile, and reduce the odor. The purified total flavonoids from chamomile can effectively scavenge DPPH and ABTS free radicals, and have strong antioxidant activity.

Key words: chamomile, total flavonoids, extract, purification, antioxidant activity

CLC Number:

TQ658

Chuanmao Li, Zhi Qiu, Fengliang Wang, Shengjie Lin, Hui Deng, Yingwei Li. Extraction and antioxidant activity of total flavonoids from chamomile[J].China Surfactant Detergent & Cosmetics, 2024, 54(9): 1039-1049.

Figures/Tables 12

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References 20

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树脂类型	性质	比表面积/（m²/g）	平均孔径/nm
DM-130	弱极性	500~550	9~10
AB-8	弱极性	480~520	13~14
D-101	非极性	550~600	9~11
NKA	非极性	570~590	20~22
NKA-9	极性	250~290	15.5~16.5

实验号	A	B	C	除脂率/%
1	1	1	1	7.104 00
2	1	2	2	7.810 00
3	1	3	3	7.540 00
4	2	1	2	8.445 45
5	2	2	3	8.414 20
6	2	3	1	8.498 48
7	3	1	3	7.681 54
8	3	2	1	7.931 00
9	3	3	2	8.390 00
K1	22.454 00	23.230 99	23.533 48
K2	25.358 13	24.155 2	24.645 45
K3	24.002 54	24.428 48	23.635 74
k1	7.484 67	7.743 66	7.844 49
k2	8.452 71	8.051 73	8.215 15
k3	8.000 85	8.142 83	7.878 58
R	0.516 18	0.308 07	0.336 57

树脂型号	吸附量/（mg/g）	吸附率/%	解吸率/%
DM-130	17.61	71.47	82.22
AB-8	20.89	84.78	86.94
D-101	19.79	80.31	78.42
NKA	19.35	78.53	80.18
NKA-9	16.86	68.42	87.91

Extraction and antioxidant activity of total flavonoids from chamomile

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水平	A	B	C
1	45	40	20
2	50	60	25
3	55	80	30