高效液相色谱法同时测定葡萄柚提取物牙膏中6种黄酮类物质

doi:10.3969/j.issn.2097-2806.2025.07.015

Abstract

Abstract:

In this study, a high performance liquid chromatography （HPLC） method was established to simultaneously detect six major flavonoids （naringin, hesperidin, neohesperidin, naringenin, eriocitrin, and tangeretin） in grapefruit extract toothpaste. The mobile phase was methanol and 0.1% formic acid aqueous solution, and the toothpaste extraction solvent was 80% methanol. The results of the methodological investigation show that the precision RSD ranges from 0.93% to 1.42%, the stability RSD ranges from 0.94% to 1.42%, and the repeatability RSD ranges from 1.14% to 1.44%, and all RSDs are less than 2.0%. The six flavonoids show a good linear relationship in the mass concentration range of 1-100 μg/mL, with a correlation coefficient of 0.999 4-0.999 8. The average recovery rate of the six flavonoids ranges from 95.20% to 104.57%, and the RSD ranges from 0.51% to 2.06%. The detection method in this study can accurately detect the content of the six flavonoids in grapefruit extract toothpaste, which has a reference value for the quality control of grapefruit extract toothpaste.

Key words: HPLC, toothpaste, grapefruit extract, flavonoids, simultaneous detection, quality control

CLC Number:

TQ658

Xiaoting Jin, Jinsheng Li. Simultaneous determination of 6 flavonoids in grapefruit extract toothpaste by high performance liquid chromatography[J].China Surfactant Detergent & Cosmetics, 2025, 55(7): 937-942.

Figures/Tables 5

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Tab. 1

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References 26

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黄酮类物质	精密度RSD/%	稳定性RSD/%	重复性RSD/%
柚皮苷	1.19	1.04	1.15
橙皮苷	1.25	1.14	1.35
新橙皮苷	1.08	1.42	1.34
柚皮素	1.40	1.35	1.27
圣草次甙	1.42	0.94	1.44
橘皮素	0.93	1.02	1.14

黄酮类物质	线性方程	相关系数	线性范围/ （μg/mL）
柚皮苷	Y=6 870 336X-5.08	0.999 7	1~100
橙皮苷	Y=7 574 356X+64.70	0.999 4	1~100
新橙皮苷	Y=6 177 452X+66.09	0.999 6	1~100
柚皮素	Y=6 635 227X-12.06	0.999 7	1~100
圣草次甙	Y=5 998 118X+30.84	0.999 8	1~100
橘皮素	Y=7 522 442X-43.91	0.999 7	1~100

黄酮类物质	加入量/（mg/g）	实测量/（mg/g）	回收率/%	RSD/%	平均回收率/%	RSD/%
柚皮苷	1	0.94	94.01	0.70	97.97	0.82
	10	10.33	103.26	1.57
	50	48.32	96.64	0.28
橙皮苷	1	1.01	100.54	0.87	95.20	1.07
	10	8.64	86.36	1.63
	50	49.35	98.70	0.90
新橙皮苷	1	1.080	108.1	0.51	104.57	2.06
	10	10.38	103.75	0.77
	50	50.92	101.84	1.18
柚皮素	1	0.95	94.98	0.80	98.08	1.02
	10	9.34	93.41	1.60
	50	52.92	105.85	0.42
圣草次甙	1	0.98	97.62	1.38	101.11	0.51
	10	9.81	98.06	0.63
	50	53.82	107.64	1.93
橘皮素	1	1.01	101.18	1.70	102.78	0.83
	10	10.17	101.71	0.75
	50	52.72	105.44	1.56

黄酮类物质	含量/（μg/g）
黄酮类物质	葡萄柚提取物牙膏1	葡萄柚提取物牙膏2	葡萄柚提取物牙膏3	葡萄柚提取物牙膏4
柚皮苷	382.98	425.56	226.59	134.34
橙皮苷	115.66	164.93	214.24	163.69
新橙皮苷	74.26	129.66	128.41	147.50
柚皮素	214.89	331.10	15.14	226.85
圣草次甙	39.73	52.23	105.59	18.65
橘皮素	78.62	102.24	149.39	69.22

Simultaneous determination of 6 flavonoids in grapefruit extract toothpaste by high performance liquid chromatography

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