复方精油-乳酸防脱育发微乳的制备工艺及药效作用研究

doi:10.3969/j.issn.2097-2806.2025.07.004

Abstract

Abstract:

This study utilizes a lactic acid solution, which promotes hair growth, as the aqueous phase, and compound essential oil as the oil phase, to prepare an O/W microemulsion for preventing hair loss and promoting hair growth. The formulation of the microemulsion was optimized using a pseudo-ternary phase diagram combined with the Box-Behnken response surface method, evaluating particle size and PDI as indicators to obtain the best preparation process. A seborrheic alopecia animal model was established using C57BL/6 mice to evaluate the effects of the microemulsion on hair loss prevention and hair growth. The results indicate that the optimal formulation of the microemulsion comprise 9.29% oil phase, 21.32% Tween 80, 10.66% anhydrous ethanol, and 58.71% aqueous phase, yielding an average particle size of 16.48 nm and a PDI of 0.038. The microemulsion exhibits good centrifugation and temperature stability. The formulation significantly increases hair length and weight in mice （P<0.05）, improves the number and distribution of hair follicles, upregulates the concentrations of KGF and VEGF in mouse skin, and decreases the concentration of TGF-β1 （P<0.05）. This study provides a basis for the development of new formulations containing essential oils for hair loss prevention and promotion.

Key words: lactic acid, essential oil of traditional Chinese medicine, microemulsion, Box-Behnken, seborrheic alopecia

CLC Number:

TQ658

Yaokun Xiong, Si Yang, Shixi Hong, Haolang Zhang, Liangji Li, Yuhuan Chen. Research on the preparation process and pharmacodynamic effect of compound essential oil-lactic acid anti-hair loss microemulsion[J].China Surfactant Detergent & Cosmetics, 2025, 55(7): 844-852.

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References 18

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因素	水平
因素	-1.414	-1	0	1	1.414
X₁/%	3.45	5.16	9.29	3.41	15.12
X₂/%	29.20	30.02	32.00	33.96	34.78

序号	因素		结果
序号	X₁（油相质量）/%	X₂（混合乳化剂质量）/%	Y₁（粒径）/nm	Y₂（PDI）
1	-1.414 21	0	20.35±0.76	0.168±0.024
2	0	0	11.04±0.14	0.092±0.030
3	1	-1	26.04±0.21	0.367±0.074
4	-1	1	22.62±0.16	0.229±0.012
5	0	0	11.72±0.14	0.087±0.026
6	0	0	16.23±0.07	0.096±0.015
7	-1	-1	13.47±0.09	0.149±0.001
8	0	0	15.96±0.04	0.080±0.005
9	1	1	22.67±0.12	0.190±0.004
10	0	1.414 21	10.89±0.05	0.192±0.011
11	0	0	12.86±0.07	0.112±0.015
12	1.414 21	0	32.97±0.75	0.311±0.024
13	0	-1.414 21	15.93±0.09	0.253±0.003

方差来源	平方和	自由度	均方	F值	P值
模型	484.18	5	96.84	13.54	0.001 7
A（油相占比）/%	116.03	1	116.03	16.22	0.005 0
B（混合相占比）/%	0.23	1	0.23	0.032	0.863 7
AB	39.19	1	39.19	5.48	0.051 8
A²	325.49	1	325.49	45.50	0.000 3
B²	0.32	1	0.32	0.045	0.837 9
残差	50.07	7	7.15
失拟项	26.96	3	8.99	1.55	0.331 5
纯误差	23.11	4	5.78
总误差	534.25	12

方差来源	平方和	自由度	均方	F值	P值
模型	0.099	5	0.020	205.35	<0.000 1
A（油相占比）/%	0.018	1	0.018	189.19	<0.000 1
B（混合相占比）/%	0.004	1	0.004	43.72	0.000 3
AB	0.017	1	0.017	171.95	<0.000 1
A²	0.038	1	0.038	393.89	<0.000 1
B²	0.030	1	0.030	308.31	<0.000 1
残差	0.001	7	0
失拟项	0.000 1	3	0	0.22	0.874 8
纯误差	0.000 5	4	0.000 1
总误差	0.099	12

粒径/nm			PDI
预测值	实际值	偏差/%	预测值	实际值	偏差/%
15.56	16.51±0.18	6.74	0.093	0.096±0.009	3.12

Research on the preparation process and pharmacodynamic effect of compound essential oil-lactic acid anti-hair loss microemulsion

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组别	第14天新生毛发长度/mm	第19天新生毛发长度/mm	第19天新生毛发重量/mg
正常组	3.87±0.47^**	7.22±0.37^**	45.6±3.6^**
2%米诺地尔组	3.51±0.28^*	7.05±0.17^**	47.3±3.9^**
脂溢性脱发模型组	2.65±0.67	6.18±0.24	31.0±5.8
26.0 mg/mL复方精油-乳酸微乳组	3.32±1.44	5.56±0.75	28.2±11.8
13.0 mg/mL复方精油-乳酸微乳组	4.56±0.86^**	6.70±0.12^**	46.0±8.6^**
6.5 mg/mL复方精油-乳酸微乳组	4.27±0.98^**	7.19±0.49^**	48.0±5.5^**

组别	平均毛囊数量/（个/视野）
正常组	57.42±4.73^**
2%米诺地尔组	51.05±5.96^**
脂溢性脱发模型组	34.21±3.42
26.0 mg/mL复方精油-乳酸微乳组	40.27±5.44^*
13.0 mg/mL复方精油-乳酸微乳组	49.33±3.98^**
6.5 mg/mL复方精油-乳酸微乳组	42.18±3.58^**

组别	ρ（KGF）/（μg/L）	ρ（VEGF）/（μg/L）	ρ（TGF-β1）/（μg/L）	ρ（DHT）/（μg/L）
正常组	1 329±385^**	1 369±184^**	98±55^**	66±49^**
2%米诺地尔组	1 301±293^**	1 140±347^**	142±42*	163±26^**
脂溢性脱发模型组	595±240	743±196	272±43	245±6
26.0 mg/mL复方精油-乳酸微乳组	1 430±567^**	963±331	192±35^**	184±51^*
13.0 mg/mL复方精油-乳酸微乳组	1 298±323^**	1 159±231^**	210±23^**	159±59^*
6.5 mg/mL复方精油-乳酸微乳组	1 003±372^*	1 106±258^*	199±38^**	158±69

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