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日用化学工业（中英文）

日用化学工业（中英文） ›› 2023, Vol. 53 ›› Issue (11): 1340-1344.doi: 10.3969/j.issn.2097-2806.2023.11.014

• 分析与检测 • 上一篇    下一篇

同位素稀释-气相色谱-质谱法测定化妆品中大麻二酚和四氢大麻酚

李长于*(),胡丹,廖上富,鹿燕,杨铭   

  1. 浙江方圆检测集团股份有限公司，浙江 杭州 310018
  • 收稿日期:2022-10-15 修回日期:2023-10-31 出版日期:2023-11-22 发布日期:2023-11-21
  • 基金资助:
    浙江省市场监督管理局雏鹰计划培育项目资助(CY2022005)

Determination of cannabidiol and tetrahydrocannabinol in cosmetics by isotope dilution-gas chromatography-mass spectrometry

Li Changyu*(),Hu Dan,Liao Shangfu,Lu Yan,Yang Ming   

  1. Zhejiang FangYuan Test Group Co., Ltd., Hangzhou, Zhejiang 310018, China
  • Received:2022-10-15 Revised:2023-10-31 Online:2023-11-22 Published:2023-11-21
  • Contact: *Tel.: +86-571-85126980, E-mail: 89087252@qq.com.

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摘要：

建立了同位素稀释-气相色谱-质谱法测定化妆品中大麻二酚（Cannabidiol，CBD）和四氢大麻酚（Tetrahydrocannabinol，THC）的检测方法。利用乙腈溶剂对水剂、乳液和膏霜类三类样品进行超声提取，经离心后得到样品溶液，以HP-5MS色谱柱（30 m×250 μm×0.25 μm）进行分离，采用全扫描和选择离子监测模式检测，同位素（四氢大麻酚-D3，Tetrahydrocannabinol-D3，THC-D3）内标法定量。结果表明，CBD和THC在0.05~1.0 mg/L质量浓度范围内均与对应的峰面积比呈良好线性关系（R2>0.995），检出限（S/N=3）分别为0.05，0.20 mg/kg，三个添加级别的加标回收率为92.0%~111.1%，相对标准偏差为0.40%~9.9%。该方法操作简单，快速，且灵敏度高，适用于化妆品中大麻二酚和四氢大麻酚的测定。

关键词: 大麻二酚, 四氢大麻酚, 气相色谱-质谱法, 化妆品, 同位素内标法

Abstract:

A method for the determination of cannabidiol （CBD） and tetrahydrocannabinol （THC） in cosmetics was established by using isotope dilution-gas chromatography-mass spectrometry （GC-MS）. The samples were ultrasonically extracted with acetonitrile, then the sample solution was prepared after centrifugation, and the analytes were separated on HP-5MS column （30 m×250 μm×0.25 μm）, detected by full-scan and selected-ion monitoring, quantified by isotope （Tetrahydrocannabinol-D3，THC-D3） internal standard method. The results show that the standard curves have good linear relationship in the range of 0.05-1.0 mg/L for CBD and THC, with the linear correlation coefficients greater than 0.995. The detection limits of the method （S/N=3） are 0.05, 0.20 mg/kg, respectively, and the spiked recoveries in three levels are between 92.0%-111.1% with the RSDs of 0.40%-9.9%. The method is simple, rapid, high sensitivity and suitable for the determination of cannabidiol and tetrahydrocannabinol in cosmetics.

Key words: cannabidiol, tetrahydrocannabinol, gas chromatography-mass spectrometry（GC-MS）, cosmetics, isotope internal standard method

中图分类号: 

  • TQ658