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日用化学工业（中英文）

日用化学工业 ›› 2020, Vol. 50 ›› Issue (12): 896-900.doi: 10.3969/j.issn.1001-1803.2020.12.014

• 分析与检测 • 上一篇    

荧光检测法快速测定化妆品中违禁成分酸性黄36

刘岱拯(),柳亚峰,谢珍茗()   

  1. 广东工业大学 轻工化工学院,广东 广州 510006
  • 收稿日期:2020-02-12 修回日期:2020-10-29 出版日期:2020-12-22 发布日期:2020-12-22
  • 通讯作者: 谢珍茗
  • 作者简介:刘岱拯（1995-）,男,广东人,硕士研究生,电话：18819892123,E-mail： 2509599941@qq.com。
  • 基金资助:
    广东省科技厅科技创意普及领域项目(2019A141405041)

Rapid fluorescence detection of prohibited cosmetic ingredient metanil yellow

LIU Dai-zheng(),LIU Ya-feng,XIE Zhen-ming()   

  1. School of Chemical Engineering and Light Industry, Guangdong University of Technology, Guangzhou, Guangdong 510006, China
  • Received:2020-02-12 Revised:2020-10-29 Online:2020-12-22 Published:2020-12-22
  • Contact: Zhen-ming XIE

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摘要：

建立了基于次氯酸氧化偶氮断裂的荧光检测法对化妆品中违禁成分酸性黄36的快速测定。该方法利用次氯酸作为荧光转移引发剂,引发酸性黄36的荧光发射信号转变来实现快速检测。通过探究反应溶剂、pH、反应时间等影响因素优化检测体系。结果表明,酸性黄36在pH=7的乙腈PBS溶液（体积比9:1）,270 nm激发波长下,345 nm处出现强荧光发射。随着次氯酸的加入,荧光发射猝灭,并于395 nm处出现强烈的荧光发射。室温下反应所需时间小于40 min。检测线性为0.2~32 μg/mL,相关系数r2=0.993 9。最低检测限为0.2 μg/mL,该方法具有良好的特异性,在真实样品中的平均回收率在90.5%~112.6%,RSD低于4.54%。

关键词: 酸性黄36, 次氯酸, 荧光检测, 化妆品

Abstract:

A fluorescence method based on hypochlorous acid azo cleavage was established for the rapid determination of metanil yellow which was a prohibited ingredient in cosmetics. Hypochlorous acid was used as a fluorescence transfer initiator to trigger the conversion of the fluorescence emission signal of metanil yellow to achieve the rapid detection. By exploring the factors such as solvent, pH and reaction time, the optimal detection system was obtained. The results showed that metanil yellow showed a strong fluorescence emission at 345 nm with an excitation wavelength of 270 nm in acetonitrile-PBS buffer （V/V=9:1） solution at pH of 7. With the addition of hypochlorous acid, the fluorescence emission was quenched and a strong fluorescence emission appeared at 395 nm. The reaction time at room temperature was less than 40 min. The linear range of detection was 0.2-32 μg/mL, and the correlation coefficient was r2=0.993 9. The limit of detection was 0.2 μg/mL, and the method had good specificity. The average recovery in real samples was 90.5%-112.6% with the RSD less than 4.54%.

Key words: metanil yellow, hypochlorous acid, fluorescence determination, cosmetics

中图分类号: 

  • TQ658