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日用化学工业（中英文）

日用化学工业 ›› 2019, Vol. 49 ›› Issue (4): 275-278.doi: 10.3969/j.issn.1001-1803.2019.04.014

• 分析与检测 • 上一篇    

胶束电动毛细管色谱法测定美白化妆品中苯酚、氢醌、熊果苷和曲酸

李乌云塔娜1,赵俊材2,王萍2,丁晓静2()   

  1. 1. 内蒙古医科大学,内蒙古 呼和浩特 010020
    2. 北京市疾病预防控制中心,北京 100013
  • 收稿日期:2018-09-14 修回日期:2019-04-03 出版日期:2019-04-22 发布日期:2019-04-24
  • 通讯作者: 丁晓静
  • 作者简介:李乌云塔娜（1983-）,女,蒙古族,内蒙古通辽人,实验师,硕士,电话：14747391983,E-mail：kindwuyuntana@163.com。
  • 基金资助:
    北京市卫生系统高层次卫生技术人才培养项目2013-2-036

Simultaneous determination of phenol, hydroquinone, arbutin, andkojic acid in whitening cosmetics by micellar electrokinetic capillary chromatography

LI Wu-yun-ta-na1,ZHAO Jun-cai2,WANG Ping2,DING Xiao-jing2()   

  1. 1. Inner Mongolia Medical University, Huhhot, Inner Mongolia 010020, China
    2. Beijing Centers for Disease Prevention and Control, Beijing 100013, China
  • Received:2018-09-14 Revised:2019-04-03 Online:2019-04-22 Published:2019-04-24
  • Contact: Xiao-jing DING

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摘要：

建立了美白化妆品中苯酚、氢醌、熊果苷和曲酸分析的胶束电动毛细管色谱法,详细研究了分离缓冲溶液的种类及各组分浓度对分离的影响。以内径50 μm、总长度40.2 cm（有效长度30 cm）未涂层熔融石英毛细管为分离柱,以110 mmol/L H3BO3+5 mmol/L Na2HPO4 （pH=7.5）+120 mmol/L十二烷基硫酸钠（SDS）为分离缓冲溶液,于5 min之内实现了4种物质的分离与测定。检测波长为214 nm。结果表明,4种组分的校正峰面积与质量浓度在12.5~2 000 μg/g范围内呈良好线性关系,相关系数均大于0.999。低、中、高3个浓度水平的加标回收率在96%~112%之间,相对标准偏差在0.08%~2.50%之间;方法的检出限（S/N=3）为2.5~12.5 μg/g。该方法准确性高、重复性好、操作方法简便,适用于实际样品的测定。

关键词: 化妆品, 胶束电动毛细管色谱法, 熊果苷, 氢醌, 曲酸, 苯酚

Abstract:

A new micellar electrokinetic capillary chromatographic method for the simultaneous determination of phenol, hydroquinone, arbutin and kojic acid in whitening cosmetics was developed. The selection and optimization of separation buffer were investigated in detail. The four substances were separated and determined within 5 minutes using uncoated fused silica capillary with an internal diameter of 50 μm and a total length of 40.2 cm （effective length of 30 cm） as separation column and 110 mmol/L H3BO3, 5 mmol/L Na2HPO4 （pH=7.5） and 120 mmol/L sodium dodecyl sulfate （SDS） as separation buffer solution. The detection wavelength was 214 nm. The results show that the corrected peak area （Ac） and the concentrations of the four components have good linear relationships within the ranges of 12.5-2 000 μg/g with the linear correlation coefficients （r） all above 0.999. The recoveries at three spiked levels are in the ranges of 96%-112%, and the relative standard deviations are in the ranges of 0.08%-2.50%. The limits of detection are in the ranges of 2.5-12.5 μg/g. The newly established method is suitable for actual sample analysis with high accuracy, good repeatability and simplicity.

Key words: cosmetics, micellar electrokinetic capillary chromatography, arbutin, hydroquinone, kojic acid, phenol

中图分类号: 

  • TQ658