微波提取白茯苓美白成分的工艺研究

doi:10.3969/j.issn.1001-1803.2019.04.007

摘要/Abstract

摘要：

以中药白茯苓为研究对象,微波辅助提取其美白成分。在提取溶剂、液料比、提取时间、微波功率单因素试验基础上,利用Box-Behnken响应面设计模型,以提取物对酪氨酸酶的相对抑制率为评价标准,应用响应面分析优化,找出了各个因素的最优组合及响应值的最佳值。确定最佳的提取工艺条件为：以水为提取溶剂,液料比为10∶1（mL ∶g）,微波功率520 W,微波时间7 min,提取2次。在该条件下提取物得率为12.70%,对酪氨酸酶的相对抑制率为31.10%,与模型理论值31.33%很接近。

关键词: 化妆品美白成分, 白茯苓, 微波提取, 酪氨酸酶, 相对抑制率, 响应面法

Abstract:

The microwave-assisted extraction of skin-whitening agent from white Poria cocos was optimized by response surface methodology. Based on single factor experiment, the extraction solvent, liquid to solid ratio, extraction time, and microwave power were selected as independent variables and the relative inhibition rate of the extractive against tyrosinase was used as the response value. The Box-Behnken design and response surface method were used to optimize the extraction technology. The optimized conditions of extraction are as follows: distilled water is used as extraction solvent, with liquid to solid ratio of 10∶1（mL∶g）, extraction time of 7 min, microwave power of 520 W, and being extracted twice in one process. Under the optimized conditions, the yield of the extractive is 12.70%, and the relative inhibition rate is 31.10%, which is close to the theoretically predicted value（31.33%）.

Key words: skin-whitening agent in cosmetics, white Poria cocos, microwave-assisted extraction, tyrosinase, relative inhibition rate, response surface method

中图分类号:

TQ658

李志英,王雪寒,高瑞苑. 微波提取白茯苓美白成分的工艺研究[J]. 日用化学工业, 2019, 49(4): 238-243.

LI Zhi-ying,WANG Xue-han,GAO Rui-yuan. Microwave-assisted extraction technology of skin-whitening agent in white Poria cocos[J]. China Surfactant Detergent & Cosmetics, 2019, 49(4): 238-243.

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参考文献 16

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反应液	V/mL
反应液	1组	2组	3组	4组
1 g/L提取物溶液	0	0	2	2
pH=6.8缓冲溶液	4	5	2	3
0.1%酪氨酸溶液	2	2	2	2
0.3 g/mL酪氨酸酶溶液	1	0	1	0
反应液总体积	7	7	7	7

水平	液料比（A）/（mL·g^-1）	提取时间（B）/ min	微波功率（C）/ W
-1	9:1	5	325
0	10:1	7	455
1	11:1	9	585

方差来源	平方和	自由度	均方	F值	P值	显著性
模型	0.011	9	1.268×10^-3	43.64	0.000 3	***
A	4.651×10^-4	1	4.651×10^-4	16.01	0.010 3	*
B	2.645×10^-4	1	2.645×10^-4	9.10	0.029 5	*
C	2.531×10^-4	1	2.531×10^-4	8.71	0.031 8	*
AB	5.523×10^-4	1	5.523×10^-4	19.01	0.007 3	**
AC	4.000×10^-6	1	4.000×10^-6	0.14	0.725 8
BC	3.803×10^-4	1	3.803×10^-4	13.09	0.015 3	*
A²	5.688×10^-3	1	5.688×10^-3	195.81	<0.000 1	***
B²	4.523×10^-3	1	4.523×10^-3	155.70	<0.000 1	***
C²	1.442×10^-4	1	1.442×10^-4	4.96	0.076 3
残差	1.452×10^-4	5	2.905×10^-5
失拟项	3.125×10^-5	3	1.042×10^-5	0.18	0.900 2	不显著
净误差	1.140×10^-4	2	5.700×10^-5
合计	0.012	1
R²=0.987 4 R_adj²=0.964 8
信噪比=22.581 CV=2.0%