防晒产品SPF值人体法与体外仪器法测定值一致性初探

doi:10.3969/j.issn.1001-1803.2019.04.006

摘要/Abstract

摘要：

使用SPF 290S防晒指数测定仪测试防晒产品体外SPF值,比较其与人体法结果的一致性。对体外仪器法测定SPF值试验条件进行研究：选择PMMA板或HD6板为基板,样品涂样量2 mg/cm ²,室温放置30 min测定,该条件下能够得到较为稳定、重复性较好的体外SPF值测定结果。对产品的研究表明无机防晒剂会影响体外法和人体法的一致性,不含无机防晒剂的防晒产品,一致性好。含无机防晒剂的样品（包括氧化锌和二氧化钛）,结果一致性较差。

关键词: 防晒化妆品, 防晒指数, 体外仪器测试

Abstract:

SPF 290S was used to test the SPF value of sunscreen products in vitro, and the consistency with the results of in vivo method was compared. The experimental conditions for the determination of SPF values by in vitro instrument were studied. PMMA plate or HD6 plate were used as the substrate, samples were coated at a dose of 2 mg/cm ², and placed in room temperature for 30 min. Under this condition, a more stable and reproducible SPF value in vitro measurement results could be obtained. Studies on the products define that inorganic sunscreen agents（including zinc oxide and titanium dioxide） can affect the consistency of in vitro and in vivo methods, and sunscreen products without inorganic show good consistency. Products containing inorganic sunscreen agents show poor consistency.

Key words: sunscreen cosmetics, SPF, in vitro instrument test

中图分类号:

TQ658

蒋珍娟. 防晒产品SPF值人体法与体外仪器法测定值一致性初探[J]. 日用化学工业, 2019, 49(4): 234-237.

JIANG Zhen-juan. Consistency of sunscreen SPF values measured by in vivo and in vitro methods[J]. China Surfactant Detergent & Cosmetics, 2019, 49(4): 234-237.

图/表 6

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参考文献 8

[1]	China Food and Drug Administration. Safety and technical standards for cosmetics (2015)[EB/OL].[ 2018- 07- 25].
[2]	Zhu Haiyan . Regulation and management of the sunscreen cosmetics market[J]. Detergent & Cosmetics, 2009,32(7) : 32-34.
[3]	Qu Xin, Zhao Xiaomin, Li Yanan , et al. Laws, tests and market trends of sunscreen cosmetics[J]. Detergent & Cosmetics, 2013,36(8) : 39-43.
[4]	Cole C . Sunscreens-what is the ideal testing model[J]. Photodermatology Photoimmunology & Photomedicine, 2014,30(2/3) : 81-87.
[5]	Nadine Cariou, Dominique Lutz . Sunscreen in-vitro SPF determination-inter and intra comparison tests between several measurement instruments[J]. Household and Personal Care Today, 2012,7(3) : 22-24.
[6]	Sebastien Miksa, Dominique Lutz, Celine Guy , et al. Improvement of in vitro sunscreen testing inter-laboratories reproducibility by means of reference absorbance curve[J]. Household and Personal Care Today, 2014,9(2) : 32-35.
[7]	Qu Xin, Zhao Xiaomin, Chen Zhihua , et al. Determination of SPF of sunscreen sold in different dosage forms by in-vitro instrumental method[J]. Detergent & Cosmetics, 2012,35(7) : 27-31.
[8]	Cao Zhi, Zhang Zhijun . Instrument assessment method for UVA protection effect of sunscreen cosmetics[J]. China Surfactant Detergent & Cosmetics, 2009,39(3) : 196-199.

防晒成分	理论含量/%	实测含量/%	偏差/%
二甲基PABA乙基己酯	7.00	7.24	3.85
二苯酮-3	3.00	3.07	2.33

基板类型	SPF值平均值	SD	CV/%
PMMA板	16.01	0.72	4.50
HD6板	18.14	1.58	8.73
3M仿肤膜	8.63	1.54	17.80
3M仿肤膜贴于石英板	14.72	2.21	15.01

SPF值测定	间隔时间/min
SPF值测定	0	5	10	15	20	30	40	50	60
测试1	16.3	15.9	15.8	15.7	15.7	15.7	15.7	15.7	15.7
测试2	16.9	16.3	16.3	16.3	16.3	16.3	16.3	16.3	16.3
测试3	16.2	15.6	15.5	15.5	15.5	15.5	15.5	15.5	15.5

放置温度	测试1	测试2	测试3	测试4	测试5	测试6
室温	16.1	16.3	16.3	16.2	15.3	16.1
37 ℃	16.5	16.5	16.3	17.3	16.1	16.3

样品涂抹量/ （mg·cm^-2）	测试1	测试2	测试3	测试4	测试5	测试6
2	16.1	16.3	16.3	16.2	15.3	16.1
1.3	13.1	14.1	12.9	14.9	13.6	13.5