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日用化学工业（中英文）

日用化学工业 ›› 2019, Vol. 49 ›› Issue (2): 132-134.doi: 10.3969/j.issn.1001-1803.2019.02.013

• 分析与检测 • 上一篇    

高效液相色谱法测定化妆品中辛酰羟肟酸的含量

李法锦,李适炜,刘瑞学,冷群英   

  1. 广东芭薇生物科技股份有限公司,广东 广州 510440
  • 收稿日期:2018-07-09 修回日期:2019-01-21 出版日期:2019-02-22 发布日期:2019-03-15
  • 作者简介:李法锦（1987-）,男,广东云浮人,电话：13928789372,E-mail：lifajin@126.com。

Determination of octyl hydroxamic acid in cosmetics by high performance liquid chromatography

Fa-jin LI,Shi-wei LI,Rui-xue LIU,Qun-ying LENG   

  1. Guangdong Bawei Biotechnology Co. Ltd, Guangzhou, Guangdong 510440, China
  • Received:2018-07-09 Revised:2019-01-21 Online:2019-02-22 Published:2019-03-15

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摘要：

建立了高效液相色谱法测定化妆品中辛酰羟肟酸含量的分析方法。采用ZORBAX Eclipse Plus C18柱（4.6 mm×250 mm,5 μm）,流动相为水-乙腈,流速1.0 mL/min,柱温25 ℃,检测波长220 nm,进样量10 μL。结果表明,辛酰羟肟酸在10~200 mg/L范围内,峰面积与质量浓度呈良好的线性关系,相关系数为0.999 9 （n=5）,方法检出限为5 mg/kg。高、中、低不同水平的加标回收率为96.28%~101.23%,相对标准偏差≤2.43%。该方法操作简便、精密度高、回收率高、重现性好,可用于化妆品中辛酰羟肟酸的检测。

关键词: 化妆品, 高效液相色谱法, 辛酰羟肟酸

Abstract:

A method for the determination of octyl hydroxamic acid in cosmetics by high performance liquid chromatography （HPLC） was established. ZORBAX Eclipse Plus C18 column （4.6 mm×250 mm, 5 μm） was used. The mobile phase was water and acetonitrile, the flow rate was 1.0 mL/min, the column temperature was 25 ℃, the detection wavelength was 220 nm and the injection volume was 10 μL. The results show that the peak area has a good linear relationship with the mass concentration of octanoyl hydroxamic acid in the range of 10-200 mg/L, and the correlation coefficient is 0.999 9 （n=5）. The limit of detection is 5 mg/kg. The recoveries of octyl hydroxamic acid at different spiked levels are 96.28%-101.23% with the relative standard deviations （RSD） less than 2.43%. This method is simple operation, high precision, high recovery and good reproducibility, and can be used to detect octyl hydroxamic acid in cosmetics.

Key words: cosmetics, high performance liquid chromatography, octyl hydroxamic acid

中图分类号: 

  • TQ658