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日用化学工业（中英文）

日用化学工业 ›› 2017, Vol. 47 ›› Issue (9): 532-535.doi: 10.13218/j.cnki.csdc.2017.09.011

• 分析与检测 • 上一篇    下一篇

高效液相色谱法检测化妆品中4种防腐剂

刘云凤, 赵井林, 张加玲, 牛娜, 乔增杰, 王明秀   

  1. 山西医科大学 公共卫生学院,山西 太原 030001
  • 收稿日期:2017-05-10 发布日期:2019-03-20
  • 通讯作者: 张加玲,教授,硕士,电话:(0351)4135167,E-mail:zhangjialing1961@163.com。
  • 作者简介:刘云凤(1984-),女,山西人,讲师,硕士,电话:(0351)4135167,E-mail:lyfsl@163.com。
  • 基金资助:
    山西医科大学青年科学基金资助项目(02201512)

Determination of four kinds of preservatives in cosmetics by high performance liquid chromatograph

LIU Yun-feng, ZHAO Jing-lin, ZHANG Jia-ling, NIU Na, QIAO Zeng-jie, WANG Ming-xiu   

  1. School of Public Health,Shanxi Medical University,Taiyuan,Shanxi 030001,China
  • Received:2017-05-10 Published:2019-03-20

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摘要： 建立了测定化妆品中4种防腐剂(脱氢乙酸、氯苯甘醚、4-羟基苯甲酸苯酯和4-羟基苯甲酸苄酯)的高效液相色谱分析方法。采用Diamonsil C18柱(250 mm×4.6 mm,5 μm),以甲醇-0.1%甲酸水溶液作为流动相进行梯度洗脱,样品经甲醇-水超声提取30 min后用紫外可见可变波长检测器检测。结果表明,4种防腐剂在12.5 min内基线分离,相关系数r＞0.998,平均回收率为88.5%～102.7%,日内精密度RSD＜3.1%,日间精密度RSD＜3.7%。该方法可用于化妆品中4种防腐剂的分析。

关键词: 化妆品, 高效液相色谱法, 防腐剂

Abstract: A method for the determination of four kinds of preservatives (dehydroacetic acid,chlorphenesin,phenyl 4-hydroxybenzoate and benzyl 4-hydroxybenzoate) in cosmetics by high performance liquid chromatography (HPLC) was established.A Diamonsil C18 column (250 mm×4.6 mm,5 μm) was used with methanol-0.1% formic acid solution as the mobile phase under the condition of gradient elution.Samples were extracted with methanol-water solution with assistance of ultrasonic wave for 30 min,and the samples were detected by VWD detector.Results show that the four kinds of preservatives are baseline separated in12.5 min.All correlation coefficients (r) are more than 0.998,with the average recoveries range from 88.5% to 102.7%.The intra-day precision (RSD) values are below 3.1% and inter-day precision (RSD) values are below 3.7%.

Key words: cosmetics, high performance liquid chromatography, preservative

中图分类号: 

  • TQ658