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日用化学工业（中英文）

日用化学工业 ›› 2015, Vol. 45 ›› Issue (5): 294-297.doi: 10.13218/j.cnki.csdc.2015.05.013

• 分析与检测 • 上一篇    

高效液相色谱法测定膏剂化妆品中的甲醛释放类防腐剂

李彦博1,2, 赵晓冬3, 陆军3, 郭春梅3, 陈晓辉1   

  1. 1.沈阳药科大学 药学院,辽宁 沈阳 110016;
    2.辽宁省食品检验检测院 理化所,辽宁 沈阳 110015;
    3.辽宁省药品检验检测院 化妆品室,辽宁 沈阳 110023
  • 出版日期:2015-05-22 发布日期:2019-04-02
  • 通讯作者: 陈晓辉,教授,电话:(024)23986259。
  • 作者简介:李彦博(1983-),男,辽宁人,硕士研究生,电话:13591497905。
       

Determination of formaldehyde releasing type anticeptics in cream type cosmetic products by high performance liquid chromatography

LI Yan-bo1,2, ZHAO Xiao-dong3, LU Jun3, GUO Chun-mei3, CHEN Xiao-hui1   

  1. 1.School of Pharmacy,Shenyang Pharmaceutical University,Shenyang,Liaoning 110016,China;
    2.Department of Physics and Chemistry,Liaoning Institute for Food Inspection and Testing,Shenyang,Liaoning 110015,China;
    3.Department of Cosmetics,Liaoning Institute for Drug Inspection and Testing,Shenyang,Liaoning 110023,China
  • Online:2015-05-22 Published:2019-04-02

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摘要： 建立了同时测定膏剂化妆品中1,3-二羟甲基-5,5-二甲基乙内酰脲(DMDMH)、1-羟甲基-5,5-二甲基乙内酰脲(1-MDMH)和5,5-二甲基乙内酰脲(DMH)的高效液相色谱法。采用Waters Atlantis T3 C18液相色谱柱(250 mm×4.6 mm,5 μm);流动相为乙腈＋0.1%甲酸溶液,流速1.0 mL/min,梯度洗脱;二极管阵列检测器,检测波长210 nm;柱温35 ℃。结果表明,DMDMH,1-MDMH和DMH质量浓度分别在17.86~595.20,17.65~588.39和18.05~601.67 mg/L范围内与相应的峰面积具有良好的线性关系,相关系数分别为0.999 5,0.999 7和0.999 8,空白样品加标回收率分别为82.6%~106.2%,98.4%~110.7%和98.6%~113.0%。

关键词: 化妆品, 高效液相色谱法, 防腐剂

Abstract: A HPLC method for simultaneous determination of 1,3-dihydroxylmethyl-5,5-dimethylhydantoin (DMDMH),1-hydroxymethyl-5,5-dimethylhydantoin (1-MDMH) and 5,5-dimethylhydantoin (DMH) in cream type cosmetic products was established.The separation is performed on a Waters Atlantis T3 C18 column (250 mm×4.6 mm,5 μm) with the mobile phase consisting of acetonitrile＋0.1% formic acid solution at flow rate of 1.0 mL/min for gradient elution.The detector is diode array type with wavelength of 210 nm.The column temperature is set at 35 ℃.Results showed that the calibration curves display good linearity within the ranges of 17.86-595.20,17.65-588.39 and 18.05-601.67 mg/L with correlation coefficient of 0.999 5,0.999 7 and 0.999 8 for DMDMH,1-MDMH and DMH respectively.The recoveries are within ranges of 82.6%-106.2%,98.4%-110.7% and 98.6%-113.0% respectively.

Key words: cosmetics, high performance liquid chromatography, anticeptics

中图分类号: 

  • TQ658