中药复方防脱液调节毛囊周期与自噬平衡发挥防脱发作用研究

doi:10.3969/j.issn.2097-2806.2026.09.005

摘要/Abstract

摘要：

探究含有侧柏叶、墨旱莲、三七提取物的中药复方防脱液（JKYF01）防脱发的作用及其潜在机制。采用干扰素-γ （IFN-γ）刺激人毛乳头细胞（hDPCs），构建体外毛囊退变模型。利用qPCR检测JKYF01对毛发生长期和退行期关键基因表达的影响，同时定量分析细胞内活性氧（ROS）含量及自噬水平。通过网络药理学探究其潜在作用机制，并结合Western blot分析核心信号通路的调控作用。IFN-γ在200 ng/mL质量浓度下可成功诱导hDPCs细胞模型，经JKYF01作用后SRY盒转录因子2 （SOX2）和肝细胞生长因子（HGF）的表达上调，同时抑制骨形态发生蛋白4和6 （BMP4/6）、转化生长因子-β2 （TGF-β2）及Runt相关转录因子2 （RUNX2）的表达。作为核心组分，侧柏叶提取物能提升SOX2和HGF的表达；而墨旱莲和三七提取物降低退行期标志物的表达。网络药理学表明，JKYF01中主要活性成分有19种，获得105个潜在治疗靶点，主要聚集在IL-6、Akt及TNF等。GO和KEGG分析显示靶点富集于PI3K/Akt信号通路、RAS信号通路、MAPK信号通路及凋亡相关通路，提示JKYF01的防脱发作用与其抗炎、调节自噬和凋亡密切相关。荧光染色结果显示JKYF01有效抑制ROS过量产生，抑制PI3K/Akt通路激活，进而促进Beclin1和LC3B的蛋白表达，提高自噬水平。JKYF01通过抑制毛囊退变、改善细胞自噬，有效调控毛囊周期和炎症微环境，发挥防脱育发的作用。

关键词: 中药复方防脱液, 毛囊周期, 自噬, 网络药理学

Abstract:

JKYF01, a self-developed patented anti-hair loss formula, consists of Biota orientalis （BO）, Eclipta prostrata （EP） and Panax notoginseng （PN） as its primary ingredients. This study aims to investigate the effects and potential mechanisms of JKYF01 in preventing hair loss. An in vitro model of hair follicle degeneration was established using human dermal papilla cells （hDPCs） stimulated with interferon-gamma （IFN-γ）. The expressions levels of critical growth indicators and catagen markers were measured by RT-qPCR. Reactive oxygen species （ROS） and autophagy levels were also quantified. Network pharmacology and experimental validation were used to delineate the underlying mechanisms. IFN-γ at 200 ng/mL successfully induces the hDPCs cell model. After treatment with JKYF01, the expression of SRY-box transcription factor 2 （SOX2） and hepatocyte growth factor （HGF） is upregulated, while the expression of bone morphogenetic proteins 4 and 6 （BMP4/6）, transforming growth factor-β2 （TGF-β2）, and runt-related transcription factor 2 （RUNX2） is inhibited. As principal ingredients, BO increases the expression of SOX2 and HGF, whereas EP and PN reduce the expression of catagen markers in a dose-dependent manner. Network pharmacology analysis indicates that JKYF01 contains 19 main active ingredients. GO and KEGG analyses show that the targets are enriched in the PI3K/Akt, RAS, and MAPK signaling pathways, as well as apoptosis-related pathways, suggesting that the hair loss-preventive effect of JKYF01 is closely related to its anti-inflammatory activity and its regulation of autophagy and apoptosis. Fluorescent staining results indicate that JKYF01 effectively inhibits excessive ROS production, and suppresses activation of the PI3K/Akt pathway, thereby promoting the expression of autophagy marker proteins Beclin1 and LC3B to enhance autophagic flux. In conclusion, JKYF01 effectively prevents hair loss by inhibiting follicle degeneration, restoring cellular autophagy, and regulating the hair follicle cycle and inflammatory microenvironment.

Key words: anti-hair loss formula of Chinese medicine, hair follicle cycle, autophagy, network pharmacology

中图分类号:

TQ658

张莹莹, 王亚琳, 赵炳天, 包丽娟, 权强华, 徐娟, 林佩. 中药复方防脱液调节毛囊周期与自噬平衡发挥防脱发作用研究[J]. 日用化学工业（中英文）, 2026, 56(9): 1169-1179.

Yingying Zhang, Yalin Wang, Bingtian Zhao, Lijuan Bao, Qianghua Quan, Juan Xu, Pei Lin. Anti-hair loss effects of a traditional Chinese medicine formula by regulating the hair follicle cycle and autophagy balance[J]. China Surfactant Detergent & Cosmetics, 2026, 56(9): 1169-1179.

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引物	正向引物	反向引物
SOX2	GCCCTGCAGTACAACTCCAT	TGGAGTGGGAGGAAGAGGTA
HGF	GACGCAGCTACAAGGGAACA	AGCTCGAAGGCAAAAAGCTG
BMP4	CACTGGCTGACCACCTCAAC	GGCACCCACATCCCTCTACT
BMP6	AACCAACCACGCGATTGTG	AAGTCTCATCGTCCCACCTC
RUNX2	CACAAGTGCGGTGCAAACTT	TGACTCTGTTGGTCTCGGTG
TGF-β2	CCTCGGGCTCAGGATAGTCT	CAGCACACTCGATATGGACCA
IL-6	CTTCCAATCTGGATTCAATGAG	GTGGTTATTGCATCTAGATTC
GAPDH	TGACTTCAACAGCGACACCCA	CACCCTGTTGCTGTAGCCAAA

药物	PubChem CID	分子名称
侧柏叶	442879	Hinokinin
	442048	Isopimaric acid
	5280863	Kaempferol
	5280343	Quercetin
墨旱莲	5280442	Acacetin
	5319744	3'-O-Methylorobol
	5281803	Pratensein
	5489605	Demethylwedelolactone
	5281813	Wedelolactone
	5280445	Luteolin
	5280343	Quercetin
三七	5280343	Quercetin
	5280794	Stigmasterol
	33934	Diop
	5282184	Mandenol
	222284	Beta-sitosterol
	9918692	Ginsenoside f2
	119307	Ginsenoside rh2
	114829	DFV