基于细胞自噬的维生素C抗黑素生成作用研究

doi:10.3969/j.issn.2097-2806.2025.01.010

摘要/Abstract

摘要：

本研究旨在探讨细胞自噬通路对维生素C（VC）抑制小鼠黑素瘤细胞（B16）中黑色素生成的调控作用。采用MTT法检测VC对B16细胞的最大安全剂量，通过加入α-促黑激素（α-MSH）诱导B16细胞的黑色素生成，再使用自噬抑制剂3-甲基腺嘌呤（3-MA）研究细胞自噬在VC的抗黑素生成作用机制。实验结果发现，VC在500 μg/mL及以下质量浓度对B16细胞存活无影响。20和50 μg/mL的VC可显著抑制α-MSH诱导的B16细胞黑色素合成（p<0.01）。与空白组相比，VC可显著诱导B16细胞中自噬相关蛋白LC3-II与p62表达上升，mTOR蛋白表达下降（p<0.05）。为进一步探究自噬对黑色素生成的影响，使用自噬抑制剂3-MA预处理B16细胞，发现3-MA阻断了VC诱导的B16细胞自噬上调，同时逆转VC的抗黑色素生成作用。本研究结果表明VC可通过激活B16细胞的自噬系统抑制黑色素生成。

关键词: 维生素C, 黑色素, 自噬, 体外试验

Abstract:

The aim of this study was to investigate the role of cellular autophagy pathway in the regulation of melanogenesis in vitamin C （VC）-inhibited mouse melanoma cells （B16）. The maximum safe dose of VC on B16 cells was detected by MTT assay, the melanogenesis was induced in B16 cells by adding α-melanotropic hormone （α-MSH）, and then the mechanism of cellular autophagy in the anti-melanogenic effect of VC was investigated by using an autophagy inhibitor （3-MA）. The results show that VC at 500 μg/mL and below have no effect on the survival of B16 cells. VC at 20 μg/mL and 50 μg/mL significantly inhibit α-MSH-induced melanin synthesis in B16 cells （p<0.01）. Compared with the blank group, VC significantly induces the expression of autophagy-related proteins LC3-Ⅱ and p62 in B16 cells, and the expression of mTOR protein decreases （p<0.05）. To further investigate the effect of autophagy on melanogenesis, B16 cells are pretreated with the autophagy inhibitor 3-MA, and it is found that 3-MA abolishes VC-induced autophagy up-regulation, as well as the anti-melanogenesis effect in B16 cells. These results suggest that VC can inhibit melanogenesis by activating the autophagy system in B16 melanoma cells.

Key words: vitamin C, melanin, autophagy, in vitro assay

中图分类号:

TQ658

张帆, 贺东琴, 卞前进, 吴凡, 秦毅, 盘瑶. 基于细胞自噬的维生素C抗黑素生成作用研究[J]. 日用化学工业（中英文）, 2025, 55(1): 83-88.

Fan Zhang, Dongqin He, Qianjin Bian, Fan Wu, Yi Qin, Yao Pan. Study on the anti-melanogenesis effect of vitamin C based on cellular autophagy[J]. China Surfactant Detergent & Cosmetics, 2025, 55(1): 83-88.

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