一种中草药复合物化妆品的祛痘抗炎功效评价研究

doi:10.3969/j.issn.1001-1803.2020.08.008

摘要/Abstract

摘要：

研究了5种中草药复合醇提取物和含有该5种中草药复合醇提物的祛痘精华液的安全性和功效性,用痤疮丙酸杆菌进行体外抑菌实验;用脂多糖LPS诱导RAW264.7小鼠巨噬细胞建立的炎症模型,进行抗炎功效测试;同时用红细胞溶血测试进行刺激性评估;最后通过含有该5种中草药复合提取物的祛痘精华液进行人体斑贴安全性测试及祛痘功效测试并与市售某款含1%水杨酸有效成分的热销祛痘产品进行对比。结果显示含有此5种中草药复合醇提物的祛痘精华液安全、温和且具有祛痘抗炎作用。

关键词: 中草药, 祛痘精华, 安全性, 功效性, 化妆品

Abstract:

The safety and efficacy of the extract from the mixture of 5 kinds of Chinese herbal medicines and the acne care essence containing this extract were studied. In vitro bacteriostasis experiment was conducted using the acne propionibacterium. Inflammatory model of with lipopolysaccharide （LPS）-induced RAW264.7 mice macrophage was set up for the anti-inflammatory efficacy test. Irritation was evaluated by RBC hemolysis test. Finally, the acne care essence containing the extract from the mixtrue of five kinds of Chinese herbal medicines was evaluated in the aspects of safety （human body patch test） and acne efficacytest, and it was also compared with a best-selling acne care product containing the active ingredient of 1% salicylic acid. The results showed that the acne care essence containing the extract from the mixtrue of five kinds of Chinese herbal medicines was safe and mild and had anti-acne effect.

Key words: Chinese herbal medicine, acne care essence, safety, efficacy, cosmetic

中图分类号:

TQ658

刘婷,刘芳,陈亮,龚盛昭. 一种中草药复合物化妆品的祛痘抗炎功效评价研究[J]. 日用化学工业, 2020, 50(8): 553-559.

LIU Ting,LIU Fang,CHEN Liang,GONG Sheng-zhao. Evaluation on the anti-acne and anti-inflammatory effects of Chinese herbal composite cosmetics[J]. China Surfactant Detergent & Cosmetics, 2020, 50(8): 553-559.

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反应程度	评分等级	皮肤反应
-	0	阴性反应
±	1	可疑红斑反应
+	2	弱阳性反应（红斑反应）：红斑、浸润、水肿,可有丘疹
++	3	强阳性反应（疱疹反应）：红斑、浸润、水肿,丘疹、疱疹;反应超出受试区
+++	4	极强阳性反应（融合性疱疹反应）：明显红斑、严重浸润、水肿、融合性疱疹;反应超出受试区

皮损类型	数量/个	皮损分值/分
无皮损	0	0
白头粉刺	≥1	1
黑头粉刺	≥1	2
炎性丘疹	≥1	3
脓头丘疹	≥1	4

受试物名称	添加量/%	菌落数量/（CFU·mL^-1）	抑菌效果评估	抑菌效果分级
中草药复合物	原液	8	极敏	极敏：菌落数≤10 CFU·mL^-1
中草药复合物	0.08%	106	中敏	高敏：菌落数10~100 CFU·mL^-1
祛痘精华液	0.08%	560	中敏	中敏：菌落数100~1 000 CFU·mL^-1
对标品	-	1 200	低敏	低敏：菌落数1 000~10⁶ CFU·mL^-1
空白对照	-	>10⁶	不敏	不敏：菌落数>10⁶ CFU·mL^-1

	祛痘精华液		对标品
	NO含量/ （μmol·L^-1）	TNF-a含量/ （ng·L^-1）	NO含量/ （μmol·L^-1）	TNF-a含量/ （ng·L^-1）
空白组	0.004±0.001	39.425± 2.041	0.006±0.002	30.563± 2.551
LPS诱导组	70.075±2.561	5 832.712±92.501	72.681±3.501	5 867.853±87.931
中草药复合物添加量为0	69.136±1.968	5 768.694±89.565	70.637±3.035	5 861.832±69.597
中草药复合物添加量为0.08%	46.623±2.697	3 102.625±61.358

样品编号	HC₅₀/ (mg·L^-1)	DI/%	L/D	刺激性分级	刺激性分级标准
中草药复合物	9 869.8	94.8	104.11	无刺激性	L/D﹥100：无刺激性 10﹤L/D≤100：微刺激性
祛痘精华液	30 102.6	98.4	2 962.10	无刺激性	1﹤L/D≤10：轻度刺激性 0.1﹤L/D≤1：中度刺激性
对标品	12 368.5	96.6	128.04	无刺激性	L/D≤0.1：重度刺激性