棕榈酰三肽-5皮肤美白功效及机制研究

doi:10.13218/j.cnki.csdc.2018.03.009

日用化学工业 ›› 2018, Vol. 48 ›› Issue (3): 166-171.doi: 10.13218/j.cnki.csdc.2018.03.009

棕榈酰三肽-5皮肤美白功效及机制研究

高雅倩¹, 唐健¹, 王志勇², 魏晓丽³, 蔡亚³

1.无限极(中国)有限公司,广东广州 510623;
2.上海医药工业研究院创新药物和制药工艺国家重点实验室,上海 200437;
3.健士星生物技术研发(上海)有限公司,上海 200335

收稿日期:2017-10-27 发布日期:2019-03-21
通讯作者: 王志勇,副研究员,博士,电话:(021)65677715,E-mail:wangzhiyong108@126.com。
作者简介:高雅倩(1984-),女,甘肃兰州人,硕士,电话:13817479169,E-mail:Lynne.Gao@infinitus-int.com。

Skin whitening efficacy and mechanism study of palmitoyl tripeptide-5

GAO Ya-qian¹, TANG Jian¹, WANG Zhi-yong², WEI Xiao-li³, CAI Ya³

1.Infinitus (China) Company Ltd.,Guangzhou,Guangdong 510623,China;
2.State Key Laboratory of New Drug & Pharmaceutical Process,Shanghai Institute of Pharmaceutical Industry,Shanghai 200437,China;
3.Healthy-Star Biological Technology Research and Development (Shanghai) Co.,Ltd.,Shanghai 200335,China)

Received:2017-10-27 Published:2019-03-21

摘要/Abstract

摘要： 采用MTT法检测细胞存活率,利用α-促黑素(α-MSH)诱导的B16小鼠黑色素瘤细胞模型和UVB照射诱导豚鼠皮肤色素沉着模型对棕榈酰三肽-5的美白功效进行评价。研究了其对细胞内酪氨酸酶活性、小眼畸形相关转录因子(MITF)和酪氨酸酶mRNA水平的影响。结果表明,棕榈酰三肽-5能够显著降低上清及细胞内的黑色素水平且无细胞毒性。进一步研究发现,棕榈酰三肽-5通过抑制酪氨酸酶活性,下调MITF和酪氨酸酶的mRNA水平,从而抑制黑色素的生成。在动物模型中,棕榈酰三肽-5能够显著降低UVB照射诱导的豚鼠皮肤色素沉着和黑色素分布区域灰度值(P<0.05)。

关键词: 美白, 棕榈酰三肽-5, 紫外线, B16细胞, 豚鼠

Abstract: To evaluate skin whitening efficacy and mechanism of palmitoyl tripeptide-5,the cell viability was detected by MTT method,B16 guinea pig melanoma cell models treated by α-melanocyte stimulating hormone (α-MSH) and hyperpigment of UVB-stimulated dorsal skin of guinea pigs were measured.Activity of tyrosinase,the mRNA expression of MITF and tyrosinase were investigated.Results show that palmitoyl tripeptide-5 exert a skin whitening efficacy by inhibiting melanin production via downregulation of MITF and tyrosinase expression,inhibiting activity of tyrosinase in melanocytes.Colorimetric analysis shows that the palmitoyl tripeptide-5 significantly inhibit the pigmentation and reduce the gray value in UVB-irradiated guinea pig dorsal skin area (P<0.05).

Key words: whitening, palmitoyl tripeptide-5, UVB, B16 cell, guinea pig

中图分类号:

TQ658

高雅倩, 唐健, 王志勇, 魏晓丽, 蔡亚. 棕榈酰三肽-5皮肤美白功效及机制研究[J]. 日用化学工业, 2018, 48(3): 166-171.

GAO Ya-qian, TANG Jian, WANG Zhi-yong, WEI Xiao-li, CAI Ya. Skin whitening efficacy and mechanism study of palmitoyl tripeptide-5[J]. China Surfactant Detergent & Cosmetics, 2018, 48(3): 166-171.

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棕榈酰三肽-5皮肤美白功效及机制研究

Skin whitening efficacy and mechanism study of palmitoyl tripeptide-5

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