中华猕猴桃水提液清除羟自由基以及抑制酪氨酸酶活性的研究

doi:10.13218/j.cnki.csdc.2018.03.008

日用化学工业 ›› 2018, Vol. 48 ›› Issue (3): 162-165.doi: 10.13218/j.cnki.csdc.2018.03.008

中华猕猴桃水提液清除羟自由基以及抑制酪氨酸酶活性的研究

张青¹, 徐勇威¹, 刘薇¹, 龚盛昭^1,2

1.广州环亚化妆品科技有限公司,广东广州 510663;
2.广东轻工职业技术学院精细化工研发中心,广东广州 510300

收稿日期:2017-11-14 发布日期:2019-03-21
通讯作者: 龚盛昭,博士,教授,主要从事精细化学工程研究。
作者简介:张青(1989-),女,湖北人,电话:(020)32098888,E-mail:zhangqing@china-huanya.com。
基金资助:
2017年广州市科技计划项目产学研协同创新重大专项(201604016111)

Study on scavenging effect for hydroxyl radicals and inhibitory effect on tyrosinase in water soluble extracts fromChinese kiwifruit

ZHANG Qing¹, XU Yong-wei¹, LIU Wei¹, GONG Sheng-zhao^1,2

1.Guahngzhou Huanya Cosmetics Technology Co.,Ltd.,Guangzhou,Guangdong 510663,China;
2.Reseach Center of Fine Chemical Technique,Guangdong Industry Technical College,Guangzhou,Guangdong 510300,China

Received:2017-11-14 Published:2019-03-21

摘要/Abstract

摘要： 以Fenton反应产生羟自由基,测定中华猕猴桃水提液清除羟自由基的能力。以L-酪氨酸和L-多巴为底物,测定中华猕猴桃水提液对酪氨酸酶单酚酶和二酚酶活力的影响以及抑制动力学。实验结果表明:中华猕猴桃水提液对羟自由基有较好的清除作用,当水提液质量浓度为1.0 g/L时,羟自由基清除率达到了95.57%。中华猕猴桃水提液对酪氨酸酶单酚酶和二酚酶的活性均有较好的抑制作用,其半数抑制浓度(IC₅₀)分别为0.28和0.35 g/L。动力学研究表明,中华猕猴桃水提液对酪氨酸酶二酚酶的抑制作用属于可逆过程,其抑制作用表现为混合型抑制。

关键词: 中华猕猴桃水提液, 羟自由基, 酪氨酸酶, 动力学

Abstract: Scavenging capacity for hydroxyl radicals in water soluble extracts from Chinese kiwifruit was detected.The hydroxyl radicals were generated from Fenton reaction.The inhibitory action of water soluble extracts from Chinese kiwifruit on the activity of monophenolase and diphenolase contained in tyrosinase were studied by using L-tyrosinase and L-DOPA as the substrates.Results show that the water soluble extracts from Chinese kiwifruit can scavenge hydroxyl radicals effectively.The hydroxyl radical elimination ratio of the water soluble extracts achieves 95.57% when the mass concentration is 1.0 g/L.The water soluble extracts from Chinese kiwifruit inhibit both monophenolase and diphenolase activities of tyrosinase effectively with IC₅₀ at 0.28 and 0.35 g/L respectively.The study of kinetics show that the inhibitory mechanism of the water soluble extracts from Chinese kiwifruit on diphenolase is reversible,and the inhibitory type is the mixed-type inhibition.

Key words: water soluble extracts from Chinese kiwifruit, hydroxyl radicals, tyrosinase, kinetics

中图分类号:

TQ658

张青, 徐勇威, 刘薇, 龚盛昭. 中华猕猴桃水提液清除羟自由基以及抑制酪氨酸酶活性的研究[J]. 日用化学工业, 2018, 48(3): 162-165.

ZHANG Qing, XU Yong-wei, LIU Wei, GONG Sheng-zhao. Study on scavenging effect for hydroxyl radicals and inhibitory effect on tyrosinase in water soluble extracts fromChinese kiwifruit[J]. China Surfactant Detergent & Cosmetics, 2018, 48(3): 162-165.

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中华猕猴桃水提液清除羟自由基以及抑制酪氨酸酶活性的研究

Study on scavenging effect for hydroxyl radicals and inhibitory effect on tyrosinase in water soluble extracts fromChinese kiwifruit

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