苯基键合硅胶萃取黄酮化合物的性能研究

doi:10.13218/j.cnki.csdc.2017.10.009

摘要/Abstract

摘要： 以苯基三氯硅烷和硅胶为原料制得苯基键合硅胶固相萃取材料,采用扫描电镜、元素分析、比表面积/孔径分析、红外光谱等对其进行了结构表征。装填固相萃取小柱,以4个黄酮模型化合物为目标分析物,研究苯基键合硅胶的萃取性能。对上样体积、上样速度、上样pH、洗脱剂类型、洗脱体积、洗脱速度等萃取参数进行了优化,并建立了苯基键合硅胶富集萃取黄酮化合物的高效液相色谱分析检测方法。实验证实该方法具有宽的线性范围(1～200 μg/L)、低的检出限(0.25 μg/L),并成功应用于2种实际样品中黄酮化合物的富集检测。上述结果说明苯基键合硅胶是一种对黄酮化合物具有良好萃取性能的固相萃取材料。

关键词: 化妆品添加剂, 黄酮化合物, 苯基键合硅胶, 固相萃取

Abstract: The phenyl bonded silica gel,as a solid-phase extraction material (the sorbent) was prepared with phenyl trichlorosilane and silica gel as raw materials.Scanning electron microscopy,elemental analysis,specific surface area/pore radius analysis and infrared spectroscopy characterized the sorbent.The phenyl bonded silica gel was packed into a solid-phase extraction micro-column and its extraction ability was examined by four model flavonoids as the analysis targets.The parameters of sample loading volume,sample loading velocity,sample solution pH value,eluent type,eluent volume and eluent velocity were assessed,and the analytical method,high performance liquid chromatography was established under the optimized conditions.Results show that the established method displays wide linearities from 1 μg/L to 200 μg/L and low limits of detection at 0.25 μg/L for the four model flavonoids.The established method was also used to analyze two real samples and satisfactory results were obtained.All the experimental results show that the prepared phenyl bonded silica gel is a good solid-phase extraction material for flavonoids.

Key words: additive of cosmetics, flavonoids, phenyl bonded silica gel, solid-phase extraction

中图分类号:

TQ658

江易扬, 王立成. 苯基键合硅胶萃取黄酮化合物的性能研究[J]. 日用化学工业, 2017, 47(10): 578-582.

JIANG Yi-yang, WANG Li-cheng. Extraction performance of flavonoids with phenyl bonded silica gel[J]. China Surfactant Detergent & Cosmetics, 2017, 47(10): 578-582.

参考文献

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