In vitro evaluation method was used to study the antibacterial and anti-inflammatory activity of extracts of Rhizoma imperataein in oral care products. Water, 50% ethanol, ethyl acetate, acetone and petroleum ether were used as solvents to extract Rhizoma imperatae, which was added to the toothpaste of silica system. The periodontitis model of 20 5-week-old SD rats was established by “cutting the gum+wire ligation+drop into the bacterial culture of periodontitis patients+feeding with high sugar sticky food”. The effects of extract from Rhizoma imperatae on periodontal pathogens [Actinobacteria （Aa）, Porphyromonas gingivalis （Pg）, Fusobacterium nucleatum （Fn）, Puccinia intermedia （Pi）, Selenium class standard bacteria （Tf）, Puccinia melanogenes （Pn）], peripheral blood, inflammatory factors in gingival crevicular fluid [C-reactive protein （CRP）, tumor necrosis factor-α （TNF-α）, interleukin-1β （IL-1β）, interleukin-6 （IL-6）, prostaglandin E2 （PGE2）], and periodontal health indicators [Plaque index （PLI）, depth of exploration （PD）, bleeding index （BI）] of the rat periodontitis model were determined, and the acute oral toxicity experiment in mice was used to verify the safety of the extracts of Rhizoma imperatae used in oral care products. The results show that the extracts of Rhizoma imperatae can significantly reduce Aa, Pg, Fn, Pi, Tf, Pn, and reduce the expression of CRP, TNF-α, IL-1β, IL-6, and PGE2. It has the activity of inhibiting periodontal pathogens, resisting periodontal inflammation, improving periodontal health, and is safe and reliable.
