构树花粗多糖对紫外线诱导小鼠皮肤光损伤的保护作用及机制研究

doi:10.3969/j.issn.2097-2806.2024.09.007

Abstract

Abstract:

To investigate the protective effect of crude polysaccharide from Broussonetia papyrifera flower on skin photodamage, a total of 56 female Kunming mice were randomly divided into 7 groups: control group, model group, matrix gel group, vitamin A group, and crude polysaccharide low-dose, medium-dose and high-dose groups. The exposed back skin of the mice was irradiated by UVA to induce skin photodamage model, and the drugs were applied to the skin 1 hour before irradiation. After 8 weeks of continuous irradiation, the back skin was scored. The effects of crude polysaccharide from Broussonetia papyrifera flower on the back skin morphology, skin thickness and collagen fibers were investigated by hematoxylin-eosin and Masson staining. And the contents of skin hydroxyproline, SOD, GSH-Px, CAT, MDA, TNF-α, IL-1β, and IL-6, and the protein expressions of MMP-1/3, Nrf2, HO-1 and NF-κB pathway were also examined. Pre-treated skin with different dose of crude polysaccharide gel 1 hour before UVA irradiation can improve roughness and ulceration of the back skin to different degrees, ameliorate skin thickening, and dermal collagen damage. At the same time, the activities of SOD, GSH-Px and CAT in skin tissue are increased, and the content of MDA is decreased （P<0.05）. The contents of pro-inflammatory cytokines, TNF-α, IL-1β and IL-6, are decreased （P<0.05）. The contents of MMP-1 and MMP-3 are decreased significantly （P<0.05）, the expression of Nrf2 and HO-1 protein are increased （P<0.01）, and p-p65/p65 protein are decreased significantly （P<0.05） in the mouse back skin by pre-treatment with crude polysaccharide gel. The crude polysaccharide from Broussonetia papyrifera flower has a strong antioxidant activity in vitro, and a protective effect on UVA-induced mice skin photodamage in vivo. The mechanism may be related to the activation of Nrf2 and inhibition of NF-κB signaling pathway, leading to the regulation of antioxidative enzyme SOD and improvement of inflammatory response.

Key words: crude polysaccharide of Broussonetia papyrifera flower, skin photodamage, oxidative stress, proinflammatory cytokine, Nrf2 pathway

CLC Number:

TQ658

Zixuan Lai, Yuxuan Song, Xuewei Duan, Shiyun Liu, Bing Liu, Minjun Zhang, Huiwen Yang. The protective effect and mechanism of crude polysaccharide from Broussonetia papyrifera flower on UV-induced skin photodamage[J].China Surfactant Detergent & Cosmetics, 2024, 54(9): 1069-1077.

Figures/Tables 12

Tab. 1

Tab. 2

Fig. 1

Fig. 2

Fig. 3

Fig. 4

Fig. 5

Fig. 6

Fig. 7

Fig. 8

Fig. 9

Fig. 10

References 26

[1]	Itoh T, Fujita S, Koketsu M, et al. Citrulluside H and citrulluside T from young watermelon fruit attenuate ultraviolet B radiation-induced matrix metalloproteinase expression through the scavenging of generated reactive oxygen species in human dermal fibroblasts[J]. Photodermatol Photoimmunol Photomed, 2021, 37 (5): 386-394.
[2]	Luo Z, Ning C, Li Z, et al. Anti-photoaging effect and mechanism of tomato and Aronia melanocarpa extract[J]. Science and Technology of Food Industry, 2023, 44 (16): 1-8.
[3]	Ivanova I, Kurz B, Lang K, et al. Investigation of the HelioVital filter foil revealed protective effects against UVA1 irradiation-induced DNA damage and against UVA1-induced expression of matrixmetalloproteinases (MMP) MMP1, MMP2, MMP3 and MMP15 [J]. Photochemical and Photobiological Sciences, 2022, 21 (3): 361-372.
[4]	Cheng X, Qian W, Chen F, et al. ATRA protects skin fibroblasts against UV-induced oxidative damage through inhibition of E3 ligase Hrd1[J]. Mol Med Rep, 2019, 20 (3): 2294-2302.
[5]	Huang K F, Ma K H, Chang Y J, et al. Baicalein inhibits matrix metalloproteinase 1 expression via activation of TRPV1-Ca-ERK pathway in ultraviolet B-irradiated human dermal fibroblasts[J]. Exp Dermatol, 2019, 28 (5): 568-575.
[6]	Ren J, Ma L. The protective effect of Nrf2 gene on ultraviolet B irradiation-induced damage in HaCaT cells[J]. Journal of Practical Dermatology, 2020, 13 (3): 131-135.
[7]	Jin Y, Chen B, Ding F, et al. Effects of ultraviolet on the expression of Hrd1 and Nrf2 in human skin fibroblasts[J]. Chinese Journal of Dermatovenereology of Integrated Traditional and Western Medicine, 2020, 19 (2): 120-125.
[8]	Wang J, Ke J, Wu X, et al. Astragaloside prevents UV-induced keratinocyte injury by regulating TLR4/NF-κB pathway[J]. Journal of Cosmetic Dermatology, 2022, 21 (3): 1163-1170.
[9]	Lee H, Kong G, Park J, et al. The potential inhibitory effect of ginsenoside Rh2 on mitophagy in UV-irradiated human dermal fibroblasts[J]. Journal of Ginseng Research, 2022, 46 (5): 646-656. doi: 10.1016/j.jgr.2022.02.001 pmid: 36090683
[10]	Xiao T, Chen Y, Song C, et al. Possible treatment for UVB-induced skin injury: Anti-inflammatory and cytoprotective role of metformin in UVB-irradiated keratinocytes[J]. Journal of Dermatological Science, 2021, 102 (1): 25-35. doi: 10.1016/j.jdermsci.2021.02.002 pmid: 33642112
[11]	Biniek K, Levi K, Dauskardt R H. Solar UV radiation reduces the barrier function of human skin[J]. Proceedings of the National Academy of Sciences of the United States of America, 2012, 109 (42): 17111-17116. doi: 10.1073/pnas.1206851109 pmid: 23027968
[12]	Park J, Yu H, Ryu H, et al. Evaluation of polyphenols from Broussonetia papyrifera as coronavirus protease inhibitors[J]. Journal of Enzyme Inhibition and Medicinal Chemistry, 2017, 32 (1): 504-515.
[13]	Shin S, Song Y, Liu K, et al. Cytotoxic activity of brous-sochalcone a against colon and liver cancer cells by promoting destruction complex-independent β-catenin degradation[J]. Food and Chemical Toxicology: an International Journal Published for the British Industrial Biological Research Association, 2019, 13 (1): 110-115.
[14]	Diana D Z, Gunt H, Levy S B. Nature-based botanical facial oil oxidative stress protection[J]. Journal of Cosmetic Dermatology, 2021, 20 (2): 522-525. doi: 10.1111/jocd.13879 pmid: 33350043
[15]	Cheng H, Huang G. Extraction, characterisation and antioxidant activity of Allium sativum polysaccharide[J]. International Journal of Biological Macromolecules, 2018, 114: 415-419. doi: S0141-8130(18)30636-6 pmid: 29596932
[16]	Kohl E, Steinbauer J, Landthaler M, et al. Skin ageing[J]. The Journal of the European Academy of Dermatology and Venereology, 2011, 25 (8): 873-884.
[17]	Hwang E, Lee D G, Park S H, et al. Coriander leaf extract exerts antioxidant activity and protects against UVB-induced photoaging of skin by regulation of procollagen typeⅠ and MMP-1 expression[J]. Journal of Medicinal Food, 2014, 17 (9): 985-995.
[18]	Liu X, Zhang R, Shi H, et al. Protective effect of curcumin against ultraviolet A irradiation-induced photoaging in human dermal fibroblasts[J]. Molecular Medicine Reports, 2018, 17 (5): 7227-7237.
[19]	Charoenchon N, Rhodes L E, Nicolaou A, et al. Ultraviolet radiation-induced degradation of dermal extracellular matrix and protection by green tea catechins: a randomized controlled trial[J]. Clinical and Experimental Dermatology, 2022, 47 (7): 1314-1323. doi: 10.1111/ced.15179 pmid: 35279873
[20]	Mostafa D K, Omar S I, Abdellatif A A, et al. Differential modulation of autophagy contributes to the protective effects of resveratrol and Co-Enzyme Q10 in photoaged mice[J]. Current Molecular Pharmacology, 2021, 14 (3): 458-468.
[21]	Heo H, Madhavan J, Eun S, et al. Pre-clinical evaluation of proprietary Lutein, Zeaxanthin, and Rosemary formulation for its dermal protective activity in male Swiss Albino Mice[J]. Preventive Nutrition and Food Science, 2021, 26 (4): 425-433. doi: 10.3746/pnf.2021.26.4.425 pmid: 35047439
[22]	Oliva N, Conde J, Wang K, et al. Designing hydrogels for on-demand therapy[J]. Accounts of Chemical Research, 2017, 50 (4): 669-679. doi: 10.1021/acs.accounts.6b00536 pmid: 28301139
[23]	Kahremany S, Hofmann L, Gruzman A, et al. NRF2 in dermatological disorders: Pharmacological activation for protection against cutaneous photodamage and photodermatosis[J]. Free Radic Biol Med, 2022, 188: 262-276.
[24]	Peng S, Shen L, Yu X, et al. miR-200a attenuated oxidative stress, inflammation, and apoptosis in dextran sulfate sodium-induced colitis through activation of Nrf2[J]. Front Immunol, 2023, 14: 1196065.
[25]	Pei X, Liang Y, Duan Y, et al. Activation of Nrf2/HO-1/NQO1 signaling pathway by Shenqi Tangluowan improves oxidative stress injury in skeletal muscle of type 2 diabetes mellitus mice[J]. Chinese Journal of Experimental Traditional Medical Formulae, 2024, 30 (7): 131-139.
[26]	Charoenchon N, Rhodes L E, Nicolaou A, et al. Ultraviolet radiation-induced degradation of dermal extracellular matrix and protection by green tea catechins: a randomized controlled trial[J]. Clinical and Experimental Dermatology, 2022, 47 (7): 1314-1323. doi: 10.1111/ced.15179 pmid: 35279873

组别	所给药物	给药量	照射情况
Control组	空白水凝胶	0.2 g/只	不照射
UVA组			UVA
UVA+N组	空白水凝胶	0.2 g/只	UVA
V_A组	维A酸乳膏	0.2 g/只	UVA
BFP低剂量组	构树花粗多糖4%凝胶	0.2 g/只	UVA
BFP中剂量组	构树花粗多糖7%凝胶	0.2 g/只	UVA
BFP高剂量组	构树花粗多糖10%凝胶	0.2 g/只	UVA

分数	皮肤变化
0分	肉色，肤质光滑
1分	肉色，出现细小皱纹
2分	皱纹明显伴轻度粗糙
3分	皱纹，粗糙严重
4分	红肿，发炎，脱屑
5分	少量溃烂，发炎，皮革状
6分	紫红溃烂，结痂

The protective effect and mechanism of crude polysaccharide from Broussonetia papyrifera flower on UV-induced skin photodamage

RichHTML

PDF (PC)

Like

Knowledge

Abstract

Cite this article

share this article

Figures/Tables 12

References 26

Related Articles 4

Metrics

Comments

Recommended 10

[1]	Meiling He,Limin Fan. Evaluation of anti-aging effect of percutaneous application of Silybum marianum extract [J]. China Surfactant Detergent & Cosmetics, 2024, 54(8): 981-987.
[2]	Wu Qi,Jin Xin,Yang Xiaoqian,Zhi Hui,Wu Nan,Zhang Hui. Pharmacodynamic study of an anti-photoaging composition and preparation of a gel mask [J]. China Surfactant Detergent & Cosmetics, 2021, 51(12): 1192-1201.
[3]	LV Ke,LI Jing,ZHANG Di. Protection of human skin cells against stress damage by use of Y₂SK₂ and SK₃ type dehydrins from Agapanthus praecox [J]. China Surfactant Detergent & Cosmetics, 2020, 50(2): 76-85.
[4]	LUO Wen-qian,HUANG Guo-meng,ZHANG Qian-hua,DENG Wen-juan,LIN Li,DU Zhi-yun. A study of alleviative effects of Chlamydomonas nivalis extract on skin damage caused by UVB [J]. China Surfactant Detergent & Cosmetics, 2019, 49(7): 441-445.