Table of Content

22 September 2023, Volume 53 Issue 9 Previous Issue    Next Issue

Basic research

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Physicochemical properties of ethoxylated sulfosuccinate surfactants and their interfacial properties when mixed with a betaine surfactant Chang Shiteng, Cai Xiaojun, Zheng Yancheng, Liu Xuejin, Yi Xiao, Jiang Zhuyang 2023, 53 (9):  989-998.  doi: 10.3969/j.issn.2097-2806.2023.09.001 Abstract ( 169 )   HTML ( 18 )   PDF (1336KB) ( 277 )   To enhance oil recovery in low-permeability reservoirs through the injection of low-mineralized water, the foaming behavior, surface tension and interfacial tension of a series of ethoxylated sulfosuccinate surfactants （C12EO6-CnMS, n=10, 12, 14 and 16） were investigated. The interfacial properties of mixtures of C12EO6-CnMS and a betaine surfactant were also studied, and the minimum alkane carbon number （Nmin） of the mixed system was obtained. The experimental results showed that C12EO6-CnMS exhibited low-foaming properties. The critical micelle concentration （cmc） of the surfactants decreased when the carbon chain length of the fatty alcohol （n） attached to ester group was increased. The addition of inorganic salts could reduce the cmc of C12EO6-CnMS, and the more inorganic salt, the more reduction of cmc. The mixed system of C12EO6-C10MS （in which the fatty alcohol carbon chain length was C10） and the betaine surfactant C16ZC displayed synergistic effect, exhibiting smaller cmc. The interfacial tension （IFT） between C12EO6-CnMS and n-alkanes increased as the carbon chain length （n） of the surfactants increased. The scanning of IFT values between C12EO6-CnMS and a series of n-alkanes was operated to determine the minimum alkane carbon number （Nmin） for the surfactant. Notably, the Nmin value increased with the addition of inorganic salts. In the presence of 0.5%-1% NaCl, the Nmin values for C12EO6-C10MS and C12EO6-C12MS are 12-14. As for surfactant mixtures, in the presence of 0.5%-1% NaCl, when the molar ratios of C12EO6-C10MS to C16ZC are 4/1, 3/2 and 2/3, the mixtures exhibited synergistic effect in reducing interfacial tension, achieving ultra-low to low IFT values （10-3-10-2 mN/m） against dodecane, tetradecane and cetane. It makes the system suitable for enhanced oil recovery （EOR） of crude oil with equivalent alkane carbon number （EACN） of 12-16. Figures and Tables | References | Related Articles | Metrics

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Differential analysis of sensitive skin surface lipids based on lipidomics Wan Zongyuan, Tong Wei, Zhou Chun, Hu Ping, Ouyang Sheng, Zhang Hongyang 2023, 53 (9):  999-1007.  doi: 10.3969/j.issn.2097-2806.2023.09.002 Abstract ( 178 )   HTML ( 23 )   PDF (1354KB) ( 298 )   This paper established a method for collecting, detecting, and analyzing the human facial skin surface lipids based on lipidomics technology for the study of lipid metabolic disorders in sensitive skin. Firstly, the young female volunteers were divided into the sensitive skin group and non-sensitive skin group by lactic acid stinging test, then the physiological indices were detected and the facial skin surface lipids were analyzed by the ultra-high performance liquid chromatography-quadrupole-time of flight mass spectrometry （UPLC-Q-TOF-MS）. Subsequently, the differential components were screened by orthogonal partial least-squares discrimination analysis （OPLS-DA） combined with t test and identified via high-resolution MS/MS, and totally 29 differential lipids of triglyceride （TG） were assigned. Compared with the non-sensitive skin group, the levels of these differential TGs are significantly reduced in the sensitive skin group, and the relevant biological significance is explained. This study provides theoretical guidance and technical support for the exploration of the pathogenesis of sensitive skin as well as the research and development of related skin care products. Figures and Tables | References | Related Articles | Metrics

Lecture of science and technology

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New emulsion system and its application in cosmetics （I）Liquid crystal emulsion system Zhou Kangfu, Yan Jiayi, Shang Yazhuo 2023, 53 (9):  1008-1017.  doi: 10.3969/j.issn.2097-2806.2023.09.003 Abstract ( 408 )   HTML ( 31 )   PDF (4080KB) ( 491 )   Liquid crystal emulsion system is a new type of emulsion system, in which the surfactant （emulsifier） molecules locate at the oil/water interface and form a long-range ordered and short-range disordered liquid crystal structure （usually lamellar liquid crystal）. The lamellar liquid crystals formed by emulsifier are similar to the skin stratum corneum lipid structure, which enable it a broad application prospect in the field of cosmetics, biomedicine, etc. With the further understanding of the liquid crystal and the gradual recognition of the advantages of liquid crystal emulsion products, the liquid crystal emulsion system has been used in high-end cosmetics widely in the past few years. In this paper, the liquid crystal and the liquid crystal emulsion system are introduced firstly and then the factors of affecting the formation of liquid crystal in emulsions are summarized. The properties of liquid crystal emulsion systems including the stability, water locking property, sustained-release property, penetration enhancing property as well as the skin sense are also provided. On this basis, the application of liquid crystal emulsion system in the cosmetic field is expounded. Furthermore, the novel liquid crystal emulsion system developed in recent years, including crystal encapsulated in liquid crystal emulsion and multiple liquid crystal emulsion are introduced. Finally, the limitations of the application of liquid crystal emulsion system in the field of cosmetics are put forward and personal views on the problems to be solved are proposed, which may provide necessary reference for the development and effective application of liquid crystal emulsion system. Figures and Tables | References | Related Articles | Metrics

Development and application

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Self-assembly of oleic acid in aqueous solution of choline hydroxide and effects of sodium dodecyl sulfate on the pH window thereof for vesicle formation Liang Xin, Xu Huifang, Lu Song, Li Yuanyuan, Wang Sijia, Xie Caixia 2023, 53 (9):  1018-1028.  doi: 10.3969/j.issn.2097-2806.2023.09.004 Abstract ( 94 )   HTML ( 7 )   PDF (2613KB) ( 148 )   Fatty acid vesicles have closed bilayer structure and can encapsulate active molecules. Taking oleic acid （OA） as model fatty acid, the phase regions including micelles, vesicles, emulsion and oil-water separation were designated using pH titration curve and visual observation method. The pH window of OA/hydroxide choline （ChOH） vesicles was determined to be 7.5-9.1. The size and morphology of OA/ChOH vesicles were characterized using dynamic light scattering, phase contrast microscopy and transmission electron microscopy, showing size polydispersity. An anionic surfactant, sodium dodecyl sulfate （SDS）, was added to OA/ChOH system, aiming to shift and expand the pH window of OA/ChOH vesicles to a range from being acidic to slightly alkaline, depending on the molar ratio （x） of SDS. At x=0.1, the pH window could be shifted to lower pH which matched the pH range of body fluid. With increasing x, the pH window was significantly broadened towards acidity, even reaching a very low pH of 1.3. The hydrogen bonding between SDS and OA molecules played a key role in the vesicle formation in the lower pH range, which had replaced the hydrogen bonding between acid-soap dimers of fatty acids. The composite vesicles as capsules had higher encapsulation efficiency, drug-loading capacity, and sustained drug release properties. This research work could provide a theoretical guidance for potential application in drug delivery and cosmetics. Figures and Tables | References | Related Articles | Metrics

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Study on the effect of lactic acid in promoting hair growth in seborrheic alopecia mice Luo Xiaojian, Hong Shixi, Luo Jing, Yang Si, Wang Ziyan, Xiong Yaokun 2023, 53 (9):  1029-1034.  doi: 10.3969/j.issn.2097-2806.2023.09.005 Abstract ( 142 )   HTML ( 17 )   PDF (2738KB) ( 171 )   The seborrheic alopecia model was established by removing the back hair of C57BL/6 mice and smearing with testosterone. The effects of lactic acid on hair growth, hair follicle growth cycle and growth factors in seborrheic alopecia mice were studied based on this model. Sixty C57BL/6 mice were randomly divided into normal group, model group, minoxidil tincture positive group, and groups of high-, medium- and low-dose lactic acid, which were smeared with corresponding drugs on the depilation area. After 24 days of continual administration, the time of skin color change, the hair growth in depilation area, the histological characteristics of hair follicles and the change of hair follicle number were recorded. The concentration of KGF, VEGF, and TGF-β1 in mouse skin was measured by corresponding Elisa kits. The results showed that, compared with the model group and normal group, lactic acid could significantly promote hair elongation in seborrheic alopecia mice, and increase the weight of mouse hair and the number of skin hair follicles in the drug-administration experimental area. Meanwhile, the concentrations of KGF and VEGF in mouse skin were up-regulated, and the concentration of TGF-β1 in mouse skin was reduced, both of which the differences were statistically significant （P<0.05）. The medium dose （10 g/L） of lactic acid solution had the best effect, which could accelerate the hair growth of mice, promote the transformation process of hair follicles in the growth period, and inhibit the symptoms of seborrheic alopecia. The curative effect of medium dose （10 g/L） of lactic acid solution was equivalent to that of minoxidil tincture （P>0.05）. It could be applied in daily care products to prevent alopecia and provide more choice for patients with seborrheic alopecia. Figures and Tables | References | Related Articles | Metrics

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Percutaneous permeation characteristics of tea polyphenols and its protective effect on human skin fibroblasts under the oxidative damage of ultraviolet A Shen Yixin, Tang Lirong, Zhang Hui 2023, 53 (9):  1035-1043.  doi: 10.3969/j.issn.2097-2806.2023.09.006 Abstract ( 136 )   HTML ( 19 )   PDF (1425KB) ( 91 )   The aim was to study the transdermal penetration characteristics of tea polyphenols and explore the protective effect of tea polyphenols on UVA-induced oxidative damage in human skin fibroblasts （HSF）. Using the Franz diffusion cell with rat skin as a barrier, a well-performed detection method was established to detect the percutaneous penetration indexes of catechin, epicatechin, epicatechin gallate and epigallocatechin gallate in tea polyphenols by high performance liquid chromatography （HPLC）. The model of HSF damaged by UVA was established to explore the effect of tea polyphenols on the content of reactive oxygen species （ROS）, the level of malondialdehyde （MDA）, the enzymatic activity of superoxide dismutase （SOD） and glutathione peroxidase （GSH-Px） in cells. The results show that the detection method has good specificity, precision and average recovery. The transdermal permeability of catechin and epicatechin increase gradually with the increase of time, and the infiltration rate of epicatechin is greater than that of catechin. However, the transdermal permeability of epicatechin galate and epigallocatechin galate can not be detected in the 24 h experiment, but the two substances remain more in the skin, indicating that the four substances in tea polyphenols can be applied to the skin well. Besides, tea polyphenols have significant protective effect on UVA-induced oxidative damage in HSF by reducing the content of ROS and the level of MDA, increasing the enzymatic activity of SOD and GSH-Px. Figures and Tables | References | Related Articles | Metrics

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Evaluation of antioxidant and protective effect against hair light damage of manganese compound Chen Liyuan, Gao Heyi, Tang Jinjing, Liu Zuohua, Liu Renlong 2023, 53 (9):  1044-1050.  doi: 10.3969/j.issn.2097-2806.2023.09.007 Abstract ( 101 )   HTML ( 5 )   PDF (1674KB) ( 86 )   The antioxidation and hair protective effect of manganese glycine and manganese sulfate by experimental model in vitro are studied. The scavenging activities of GlyMn and MnSO4 on superoxide free radicals, hydrogen peroxide and hydroxyl free radicals were determined, and the content of GlyMn and MnSO4 on reactive oxygen free radicals was investigated to evaluate their antioxidant effects. The content of free radicals produced by the degradation of melanin in hair was measured by oxidative damage test, and the photoprotective effect of melanin on hair surface was evaluated by SEM. The function of GlyMn and MnSO4 shampoo was evaluated by human efficacy test. The results show that GlyMn and MnSO4 have significant scavenging effects on the three kinds of free radicals, among which 3 mmol/L manganese compound can significantly remove superoxide free radicals, which is equivalent to 3 U/mL manganese superoxide dismutase. When the concentration of manganese compound is 50 mmol/L, it can significantly reduce the free radicals produced by melanin degradation in hair. It can effectively protect the light damage of hair and protect the hair scales from damage, showing obvious antioxidant and light-damaged hair protection effect. Figures and Tables | References | Related Articles | Metrics

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Preparation and performance study of zinc oxide nanocomposite antibacterial materials Chang Xiangling, Chai Fei 2023, 53 (9):  1051-1056.  doi: 10.3969/j.issn.2097-2806.2023.09.008 Abstract ( 132 )   HTML ( 4 )   PDF (2372KB) ( 68 )   Zinc oxide, as a photocatalytic antibacterial material, has attracted much attention due to its low cost and high safety. In this paper, a series of TiO2-ZnO composite antibacterial materials with different TiO2 mole doping （0%, 5%, 10%, 15%, 20%） were prepared by ultrasonic calcination method. The results show that TiO2-ZnO composite is composed of hexagonal wurtzite ZnO and anatase TiO2. The addition of TiO2 generates new Zn-O-Ti bonds. TiO2 particles are continuously and evenly distributed on the surface of ZnO, and the size of ZnO is 2-3 μm. The colony of Escherichia coli （E. coli） cultured for 12 h shows that the antibacterial ability of pure ZnO is weak. With the increase of TiO2 addition, the number of colonies decreases, and the antibacterial ability of TiO2-ZnO gradually increases. The antibacterial ability of TiO2 ZnO composite antibacterial material with TiO2 doping mole ratio of 15% is the strongest. Under the photocatalytic reaction, the sterilization efficiency of TiO2-ZnO doped with 15% mole ratio of TiO2 reaches 100% in 15 min. TiO2-ZnO rapidly destroy the cell membrane of Escherichia coli under the light, causing serious deformation of Escherichia coli and the outflow of cytoplasm, thus killing bacteria. The addition of TiO2 significantly improves the sterilization ability of ZnO antibacterial materials. Figures and Tables | References | Related Articles | Metrics

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Synergistic effect of silk peptides thermophilus fermentation on the skin anti-inflammatory and anti-aging activities Fang Tinghuan, Zheng Ting, Jiang Qing, Li Xiaoxia, Tang Lirong 2023, 53 (9):  1057-1064.  doi: 10.3969/j.issn.2097-2806.2023.09.009 Abstract ( 157 )   HTML ( 17 )   PDF (2117KB) ( 95 )   In order to study the synergistic effects of silk peptide fermented by thermophilus on skin anti- inflammatory, barrier moisturizing and anti-aging activities, silk peptides thermophilus fermentation was prepared referring to the method of microbial fermentation, and lipopolysaccharide （LPS） -induced keratinocytes （HaCaT cells） and hydrogen peroxide （H2O2） -induced fibroblasts （HDF cells） were used as research models. The inhibition rates of cell proliferation, the contents of interleukin 1β （IL-1β）, interleukin 6 （IL-6）, aquaporin 3 （AQP3）, filaggrin （FLG）, superoxide dismutase （SOD）, advanced glycosylation end products （AGEs） and human collagen I alpha 1 （COL-1） were detected by colorimetric assay, enzyme-linked immunosorbent assay （ELISA）, et al. The results show that silk peptide fermentation can significantly reduce the expression of IL-1β, IL-6 and AGEs, and can significantly promote the expression of FLG, AQP3, SOD and COL-1. Especially the silk peptide fermentation can more significantly inhibit the expression of IL-6 and AGEs, and can more significantly promote the migration of HDF cells and SOD synthesis than the single silk peptide. And also the silk peptide fermentation can more significantly reduce the expression of IL-1β, IL-6 and AGEs, and can more significantly promote the expression of AQP3, HDF cell migration and SOD synthesis than the single fermentation of thermophilic bacteria. Compared with the single silk peptide and the fermentation of thermophilus, the silk peptide fermentation has obvious synergistic effects of anti-inflammatory, barrier moisturizing and anti-aging activities. Figures and Tables | References | Related Articles | Metrics

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The study of Magnolia biondii flower extract in relieving UVB-induced skin damage Huang Fang, Zhang Ying, Xiong Yue, Zhou Lidan, Lu Yina 2023, 53 (9):  1065-1072.  doi: 10.3969/j.issn.2097-2806.2023.09.010 Abstract ( 159 )   HTML ( 5 )   PDF (4138KB) ( 118 )   Magnolia biondii flower extract （MB）, which is rich in magnoliol was used to study its efficacy on alleviating UVB-induced skin damage. Immunofluorescence staining and enzyme-linked immunosorbent assay were applied to detect different key biomarkers on UVB-induced keratinocytes （HaCaT） and vascular endothelial cells （HUVEC）, which induced by HaCaT cell culture supernatant after UVB stimulated for 48 h. The results show that the levels of cyclobutane pyrimidine dimer （CPD） and high mobility group box protein B1 （HMGB1） increase significantly in HaCaT cells from 16 h to 72 h after UVB irradiation, and also HMGB1 is gradually migrated from the nucleus to the cytoplasm. The expression of heat shock family protein 27 （Hsp27） also increases, as well as the cytokines such as interleukin 6 （IL-6）, interleukin 8 （IL-8）, and prostaglandin E2 （PGE-2）. Meanwhile, the expression levels of endothelin-1 （ET-1） and intercellular adhesion molecule-1 （ICAM-1） are significantly increased after HUVEC cells stimulated by cell culture supernatant for 24 h. Under the synchronous treatment of 0.1%-0.5% MB, the levels of CPD and HMGB1 in HaCaT cells are significantly inhibited, with an increase expression of Hsp27, and a decreased of the nuclear transfer of HMGB1 and cytokines. In HUVEC cells, the expression levels of ET-1and ICAM-1 are also significantly inhibited after MB treatment. Therefore, MB can alleviate the damage of skin after UV irradiation by reducing DNA damage, reducing the synthesis and nuclear transfer of HMGB1, and inhibiting the expression of cytokines and vascular permeability. Figures and Tables | References | Related Articles | Metrics

Reviews

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Research progress in skin physiological effects and its cosmetic encapsulation technology of ferulic acid Xiong Jie, Yang Dan, Meng Hong, He Yifan, Pei Xiaojing 2023, 53 (9):  1073-1079.  doi: 10.3969/j.issn.2097-2806.2023.09.011 Abstract ( 159 )   HTML ( 9 )   PDF (1259KB) ( 140 )   Ferulic acid has good skin physiology effects such as whitening, anti-aging and sun protection, and can be used as a raw material in cosmetics. At present, ferulic acid is widely used in medicine and food, and it is still in the research and development stage in cosmetics. This review mainly elaborated the physiological activities of ferulic acid such as whitening, anti-aging, sunscreen and its application in cosmetics. Due to the poor solubility in water and the unstable properties of ferulic acid, its application in cosmetics is limited. For this reason, many efforts have been made to improve the applicability of ferulic acid. Among them, the encapsulation technology is to wrap ferulic acid and then apply it to cosmetics, which can not only improve the solubility of ferulic acid, but also increase the compatibility of ferulic acid in cosmetic formulations. This paper summarizes several commonly used encapsulation technologies, including phospholipid complexes, nanoemulsion, cyclodextrin inclusion complexes, hydrogels, molecular cages, etc., which provide significant references for the development and application of ferulic acid in cosmetics. Figures and Tables | References | Related Articles | Metrics

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Discussion of cosmetic ingredient management and regulatory system of cosmetic ingredient safety information submission He Miao, Su Zhe, Hu Kang, Zhang Fenglan, Wang Gangli, Lu Yong 2023, 53 (9):  1080-1086.  doi: 10.3969/j.issn.2097-2806.2023.09.012 Abstract ( 128 )   HTML ( 9 )   PDF (1050KB) ( 106 )   In order to implement the Regulations on Supervision and Administration of Cosmetics （CSAR） and to improve the ingredient management and safety evaluation level of cosmetics, the National Medical Products Administration （NMPA） has established a system for submitting safety information of cosmetic ingredients, in combination with the drafting and revision of the supporting laws and regulations and the work of information system construction. Focused on this work, the authors analyze the general situation and related issues of the cosmetic ingredient management under the previous background of regulation and supervision, and introduce the general thoughts and arrangement under the new regulatory system of CSAR, so as to explain in detail the whole design and mechanisms of the safety information submission of cosmetic ingredients. Finally, based on practical feedback, the authors also discuss some key issues of common concern in the industry, such as the principle of composition identification and division, the concept of typical value/target value and range value of components, change of ingredient safety information, and the signature requirement of ingredient safety information documents. With these discussions, the work of safety information submission of cosmetic ingredients is reviewed and summarized, and some technical suggestions can also be provided. Figures and Tables | References | Related Articles | Metrics

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Research status of network pharmacology and its application prospect in the field of cosmetics Sun Jinyue, He Congfen 2023, 53 (9):  1087-1093.  doi: 10.3969/j.issn.2097-2806.2023.09.013 Abstract ( 167 )   HTML ( 14 )   PDF (1773KB) ( 128 )   The emergence of cyberpharmacology is the result of the current development of artificial intelligence and big data era, which plays a very important role in the treatment of diseases and the development of new drugs in the field of medicine, and is currently mainly used to reveal the mechanism of action of drugs in the body to treat diseases and guide the use of drugs. The mechanism of action on the skin is similar to the action of Chinese medicine on the body in the field of medicine, but there is no research on the application of network pharmacology in the field of cosmetics. In this paper, we review the current development of applied research in cyberpharmacology from the aspects of pharmacology and Chinese medicine research, complex disease discovery, and drug creation, and propose the future application of cyberpharmacology in the selection of cosmetic botanical efficacy ingredients, cosmetic efficacy evaluation, and cosmetic safety evaluation. It is pointed out that skin-related targets, such as sensitive skin-related inflammatory targets: TNF-α, IL-1α, IL-1β, IL-8, TRPV1; whitening-related targets: TYR, TRP-1, TRP-2; acne-related anti-inflammatory and antibacterial targets, etc., can be used to screen herbal ingredients for cosmetic products and guide the development of formulations to achieve precise skin care. Network pharmacology can also be applied to the phyto-effective ingredients in the existing formulations to explore the mechanism of action, efficacy evaluation and safety evaluation. This paper presents insights and operational steps for the application of network pharmacology in the above aspects, with the aim of providing new scientific and technological support for the development of cosmetics. Figures and Tables | References | Related Articles | Metrics

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Optimal design method for packaging elements of daily skin care products under visual effects Gu Weiwei 2023, 53 (9):  1094-1100.  doi: 10.3969/j.issn.2097-2806.2023.09.014 Abstract ( 91 )   HTML ( 2 )   PDF (1453KB) ( 70 )   To improve the visual effect of daily skincare packaging, research is conducted on the optimization design method of packaging elements for daily skincare products under visual effects. Firstly, describe the visual effects to illustrate their impact on consumers’ consumption behavior. Secondly, from four aspects: color elements, graphic elements, styling elements, and textual elements, consider the visual effects of consumers to design daily skincare product packaging. Finally, fully consider the principles of packaging design such as uniformity, contrastive prominence, and symmetry, and optimize the packaging design of daily skincare products through the combination of elements such as separation, overlap, and overlay. The results indicate that the proposed method is effective and can improve the visual effect of cosmetic packaging. It aims to bring consumers a pleasant shopping experience and good visual effect through packaging of daily skincare products. Figures and Tables | References | Related Articles | Metrics

Analytical methods and testing

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Study on the standardization of evaluation for the efficacy of toothpaste in extrinsic stain removal Wang Jicai, Jian Ruidong, Xie Yu, Chen Xiaobin, Zhou Yi, Tan Jianhua 2023, 53 (9):  1101-1107.  doi: 10.3969/j.issn.2097-2806.2023.09.015 Abstract ( 120 )   HTML ( 4 )   PDF (1167KB) ( 63 )   A standardized in vitro method was established to evaluate the efficacy of toothpaste in extrinsic stain removal. Using bovine incisors as substrates and L*a*b* color space as tooth color evaluating system, the removal effects of toothpaste samples on extrinsic stains were determined through the process of dyeing-brushing-testing. The critical parameters of the method were systematically optimized. A standardized method was established in which an L8-Ⅱ brushing machine was equipped with flat medium-bristle toothbrush and the dyed bovine incisors with L* in the range of [40, 50] were chosen to be brushed for 600 times at 100 r/min. The results showed that the established method could effectively distinguish the difference between samples. The relative standard deviation （RSD） between experimental batches was less than 10% and the results were reproducible. This method was low-cost, short-period and easily operated. It might provide reliable and scientific test results for the efficacy of whitening and stain-removed toothpastes. Figures and Tables | References | Related Articles | Metrics

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Determination of thiazolidinones in oral care products by ultra performance liquid chromatography Song Qingmei, Du Feng 2023, 53 (9):  1108-1114.  doi: 10.3969/j.issn.2097-2806.2023.09.016 Abstract ( 124 )   HTML ( 10 )   PDF (1075KB) ( 88 )   The purpose of this paper is to determine the content of isothiazolinones in oral care products by ultra performance liquid chromatography. A HyperSep reversed-phase C8/BSAIE ion exchanger, solid phase extraction （SPE） with Sep-Pak C18 sorbent and an ultra performance liquid chromatography/diode array detector （UPLC/DAD） were used for the simultaneous determination of methylisothiazolinone （MI） and methylchloroisothiazolinone （MCI） in oral care products. The results find that the eluting solvents for HyperSep C8/BSAIE and UPLC/DAD are mixtures （acetonitrile/methanol, volume ratio 2:1） with an optimum detection wavelength of 255 nm. The method has a good linear range （0.005-10 µg/mL）, the coefficient of determination （R2） ranges from 0.997 to 0.999, and the limit of detection （LOD） is in the range of 0.001-0.002 µg/g. The relative standard deviation RSD is less than 3% for the detection of standard mixtures at low （0.07 µg/mL）, medium （3 µg/mL） and high （15 µg/mL） concentrations. A total of 12 oral care samples are studied, with the reaching concentrations of nd-0.89 µg/g for MI and nd-0.62 µg/g for MCI. Recoveries range from 95.33% to 101.43% depending on the type of sample. Figures and Tables | References | Related Articles | Metrics