荷叶提取物在抑制口腔细菌和改善牙龈炎症的有效性分析

doi:10.3969/j.issn.2097-2806.2024.10.011

Abstract

Abstract:

This study aimed to analyze the effectiveness of Lotus leaf extract （LLE） in inhibiting oral bacteria and improving gingival inflammation. LLE of different mass concentrations was used to treat 10 common oral bacteria for 24 hours, and the minimum inhibitory concentration （MIC） and inhibition zone diameter were measured respectively. The CCK-8 method was used to detect the effect of LLE on the viability of human gingival epithelial cells. Human gingival epithelial cells were divided into control group （Control）, lipopolysaccharides （LPS） group and LPS+1-500 μg/mL LLE group. Human gingival epithelial cells were treated with 1 μg/mL LPS and different mass concentrations of LLE respectively for 24 h. RT-qPCR were used to detect the mRNA levels of interleukin （IL）-1β, IL-6, IL-8, tumor necrosis factor-α （TNF-α）, and intercellular adhesion molecule-1 （ICAM-1）. Western blotting were used to detect the phosphorylation levels of nuclear factor-κB （NF-κB） p65 and p38 mitogen-activated protein kinase （MAPK）. The results show that the MICs of LLE against 10 common oral bacteria are 300-500 μg/mL, respectively. Compared with the Control group, the relative mRNA levels of IL-1β, IL-6, IL-8, TNF-α and ICAM-1 in human gingival epithelial cells in the LPS group are all increased, and the phosphorylation levels of NF-κB p65 and p38 MAPK are all increased （P<0.05）. Compared with the LPS group, the relative mRNA levels of IL-1β, IL-6, IL-8, TNF-α and ICAM-1 in human gingival epithelial cells in the LPS+50-500 μg/mL LLE group are all decreased in a dose-dependent manner, and the phosphorylation levels of NF-κB p65 and p38 MAPK are decreased in a dose-dependent manner （P<0.05）. This study shows that LLE has inhibitory activity against 10 common oral bacteria and LPS-induced excessive inflammation of human gingival epithelial cells, and it may be an effective ingredient in oral care products.

Key words: Lotus leaf extract, oral bacteria, gingival inflammation, human gingival epithelial cells, nuclear factor-κB

CLC Number:

TQ658

Juanli Liu, Huiping Xu, Yanfang Liu, Zhenhua Dong, Meiyan Wang. Analysis of the effectiveness of Lotus leaf extract in inhibiting oral bacteria and improving gingival inflammation[J].China Surfactant Detergent & Cosmetics, 2024, 54(10): 1235-1242.

Figures/Tables 7

Tab. 1

Fig. 1

Tab. 2

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References 27

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Gene	Primer sequence
IL-1β	F: 5′-TACAAGGAGAAGAAAGTAATGACAA-3′
IL-1β	R: 5′-AGCTTGTTATTGATTTCTATCTTGT-3′
IL-6	F: 5′-AGCCACTCACCTCTTCAGAACG-3′
IL-6	R: 5′-GGTTCAGGTTGTTTTCTGCCAG-3′
IL-8	F: 5′-CTTGGCAGCCTTCCTGATTTC-3′
IL-8	R: 5′-TTCTGTGTTGGCGCAGTGTG-3′
TNF-α	F: 5′-CTCCTCACCCACACCATCAGCCGCA-3′
TNF-α	R: 5′-ATAGATGGGCTCATACCAGGGCTTG-3′
ICAM-1	F: 5′-AGAACCTTACCCTACGCTGC-3′
ICAM-1	R: 5′-CAGTATTACTGCACACGTCAGC-3′
GAPDH	F: 5′-GGAGCGAGATCCCTCCAAAAT-3′
GAPDH	R: 5′-GGCTGTTGTCATACTTCTCATGG-3′

ρ（LLE）/（μg/mL）	抑菌圈直径/mm
ρ（LLE）/（μg/mL）	F. nucleatum	E. coli	P. aeruginosa	E. faecalis	S. mutans	E. faecium	S. oralis	P. gingivalis	A. oris	C. albicans
0	0.00±0.00	0.00±0.00	0.00±0.00	0.00±0.00	0.00±0.00	0.00±0.00	0.00±0.00	0.00±0.00	0.00±0.00	0.00±0.00
1	0.00±0.00	0.00±0.00	0.00±0.00	0.00±0.00	0.00±0.00	0.00±0.00	0.00±0.00	0.00±0.00	0.00±0.00	0.00±0.00
10	0.00±0.00	0.00±0.00	0.00±0.00	0.00±0.00	0.00±0.00	0.00±0.00	0.00±0.00	0.00±0.00	0.00±0.00	0.00±0.00
50	6.14±0.25	5.91±0.29	0.00±0.00	0.00±0.00	5.94±0.24	0.00±0.00	6.88±0.38	0.00±0.00	5.92±0.44	0.00±0.00
100	8.27±0.35	8.34±0.34	6.98±0.26	5.71±0.28	7.74±0.32	6.41±0.23	9.00±0.23	5.01±0.26	8.17±0.38	5.12±0.32
200	10.96±0.47	10.82±0.52	8.95±0.44	8.50±0.31	9.83±0.39	8.63±0.48	11.66±0.49	7.11±0.31	10.34±0.42	6.99±0.31
300	14.88±0.83	14.46±0.49	10.86±0.56	11.72±0.61	13.17±1.06	11.83±0.36	15.94±0.48	9.00±0.44	13.08±0.89	10.00±0.36
400	17.82±0.46	16.48±1.10	15.99±1.16	15.20±0.75	17.35±0.80	16.41±0.89	18.96±0.83	11.83±0.38	17.03±0.48	13.61±0.70
500	19.75±1.05	20.06±1.34	19.12±1.09	18.15±0.80	22.36±1.72	19.36±0.71	21.52±1.03	15.80±0.85	21.00±0.82	17.40±0.52
F	1 351.092	840.555	960.773	1 612.197	692.052	1 833.000	1 553.356	1 561.894	1 462.331	2 170.473
P	<0.001	<0.001	<0.001	<0.001	<0.001	<0.001	<0.001	<0.001	<0.001	<0.001

Analysis of the effectiveness of Lotus leaf extract in inhibiting oral bacteria and improving gingival inflammation

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