表面活性剂对PCMX抑菌效果影响研究

doi:10.3969/j.issn.2097-2806.2024.10.008

Abstract

Abstract:

The solubilization effects of three surfactants, i.e., SDS, AES and AEO9, on PCMX （parachlorometaxylenol） were studied, as well as their combined impact on the antibacterial activity against Staphylococcus aureus, Escherichia coli, and Pseudomonas aeruginosa. The results indicated that, the binding coefficient of surfactant with PCMX was influenced by the concentration of surfactant in the following order: AEO9>AES>SDS. The influences of surfactants on the antibacterial effect of PCMX were examined through minimum inhibitory concentration （MIC） testing. For the SDS concentrations below its critical micelle concentration （cmc）, the MIC of the PCMX complex remained unchanged. However, a further increase in SDS concentration beyond the cmc would lead to a linear increase in MIC. In the case of AES below cmc, the MIC of the PCMX complex was slightly decreased with the concentration of AES, while the AES in the concentration range above cmc would cause a linear increase of MIC. When the concentration of AEO9 was≤1.61 mmol/L, the increase of AEO9 concentration would lead to a decrease in MIC against Staphylococcus aureus, Escherichia coli, and Pseudomonas aeruginosa. Above the cmc of AEO9, there was a concentration range in which the increase of AEO9 concentration could enhance the antibacterial efficacy of the system. This study might provide valuable insights into the synergism between surfactants and PCMX in antibacterial effectiveness.

Key words: PCMX, surfactant, critical micelle concentration, minimum inhibitory concentration, binding coefficient

CLC Number:

TQ423

Qinggang Liu. Study on the influences of surfactants on the antibacterial effect of PCMX[J].China Surfactant Detergent & Cosmetics, 2024, 54(10): 1211-1217.

Figures/Tables 12

Tab. 1

Fig. 1

Tab. 2

Tab. 3

Fig. 2

Tab. 4

Fig. 3

Tab. 5

Tab. 6

Tab. 7

Tab. 8

Fig. 4

References 13

[1]	Bai Jianyun. Performance evaluation of antibacterial agents in laundry detergents[J]. Detergent & Cosmetics, 2022, 45 (8) : 37-39.
[2]	Deluca P P, Kostenbauder H B. Interaction of preservatives with macromolecules IV. Binding of quaternary ammonium compounds by nonionic agents[J]. Journal of Pharmaceutical Sciences, 2010, 49 (7) : 430-437.
[3]	Pogorzelski S, Watrobska-Swietlikowska D, Sznitowska M. Surface tensometry studies on formulations of surfactants with preservatives as a tool for antimicrobial drug protection characterization[J]. Journal of Biophysical Chemistry, 2012, 3 (4) : 324-333.
[4]	Pisano F D, Kostenbauder H B. Interaction of preservatives with macromolecules Ⅱ. Correlation of binding data with required preservative concentrations of p-hydroxybenzoates in the presence of Tween 80[J]. Journal of the American Pharmaceutical Association (Scientific ed.), 2010, 48 (6) : 310-314.
[5]	Huang J H, Zhao Y, Zeng G M, et al. Micellar-enhanced ultrafiltration for the solubilization of various phenolic compounds with different surfactants[J]. Water Science & Technology, 2015, 72 (4) : 623-631.
[6]	Gilbert P H, Zhang Z, Qian K K, et al. Aggregation kinetics of polysorbate 80/m-cresol solutions: a small-angle neutron scattering study[J]. Mol. Pharm., 2022, 19 (3) : 862-875.
[7]	Gilbert P H, Zhang Z, Qian K K, et al. Preservative induced polysorbate 80 micelle aggregation[J]. J. Pharm. Sci., 2021, 110 (6) : 2395-2404.
[8]	Zhang Jiajia, Zhang Hongqiang, Wang Jiahui, et al. Effect of surfactant system on the properties of antimicrobial agents[J]. China Cleaning Industry, 2022 (9) : 57-61.
[9]	Dorman H J D, Deans S G. Antimicrobial agents from plants: antibacterial activity of plant volatile oils[J]. J. Appl. Microbiol., 2000, 88 (2) : 308-316. doi: 10.1046/j.1365-2672.2000.00969.x pmid: 10736000
[10]	Guo Yi, Gao Jingfeng, Wang Zhiqi, et al. The fate and behavior mechanism of antibiotic resistance genes and microbial communities in flocs, aerobic granular and biofilm sludge under chloroxylenol pressure[J]. Journal of Hazardous Materials, 2022 (438) : 1-14.
[11]	Correia E L, Brown N, Ervin A, et al. Contamination in sodium dodecyl sulfate solutions: insights from the measurements of surface tension and surface rheology[J]. Langmuir, 2022, 38 (23) : 7179-7189.
[12]	Schmolka I R. The synergistic effects of nonionic sur-factants upon cationic germicidal agents[J]. Journal of Cosmetic Science, 1973 (24) : 577-592.
[13]	Tobe S, Majima T, Tadenuma H, et al. Nonionic surfactants enhancing bactericidal activity at their critical micelle concentrations[J]. J. Oleo. Sci., 2015, 64 (1) : 61-68. doi: 10.5650/jos.ess14159 pmid: 25492231

表面活性剂类型	cmc/（mmol/L）
表面活性剂类型	纯水	PCMX溶液（c=1.28 mmol/L）
SDS	7.98	5.89
AES	2.58	2.06
AEO9	0.177	0.017 7

c（SDS）/（mmol/L）	c（PCMX）/（mmol/L）	U
0	2.33	1.00
2.77	2.79	1.20
8.67	5.40	2.32
17.34	13.00	5.57
26.01	19.90	8.58
34.68	26.90	11.55
52.01	38.80	16.70
69.35	51.20	22.02
138.71	98.30	42.27
208.06	149.00	63.88
277.41	205.00	88.24
346.76	278.00	119.32

c（AES）/（mmol/L）	c（PCMX）/（mmol/L）	U
0	2.33	1.00
1.31	4.30	1.85
6.54	8.99	3.86
13.09	15.20	6.56
19.63	20.60	8.87
26.18	26.70	11.46
39.27	37.90	16.28
52.36	50.40	21.69
104.71	92.90	39.96
157.07	133.00	57.28
209.42	175.00	75.24
261.78	215.00	92.50

c（AEO9）/（mmol/L）	c（PCMX）/（mmol/L）	U
0	2.33	1.00
0.32	2.28	0.98
1.61	2.61	1.12
3.22	3.81	1.64
8.05	9.02	3.88
16.10	14.60	6.28
24.15	21.50	9.24
32.21	27.90	12.00
64.41	58.10	24.98
96.62	89.50	38.46
128.82	132.00	56.59
161.03	177.00	76.24

表面活性剂	MIC/（mmol/L）
表面活性剂	金黄色葡萄球菌	大肠杆菌	绿脓杆菌
SDS	0.520~0.347	>178	>178
AES	1.030~0.515	>132	>132
AEO9	>90.5	> 90.5	> 90.5

Study on the influences of surfactants on the antibacterial effect of PCMX

RichHTML

PDF (PC)

Like

Knowledge

Abstract

Cite this article

share this article

Figures/Tables 12

References 13

Related Articles 15

Metrics

Comments

Recommended 10

c_SDS/（mmol/L）	MIC/（mmol/L）
c_SDS/（mmol/L）	大肠杆菌	绿脓杆菌
0	0.639	7.99
2.77	0.639	7.99
8.67	1.920	10.20
17.34	4.790	12.80
26.01	5.430	16.00
34.68	7.990	19.20
52.01	9.580	24.30
69.35	12.800	30.70
138.71	24.000	53.70
208.06	36.700	76.70
277.41	43.100	102.00
346.76	57.500	121.00

c_AES/（mmol/L）	MIC/（mmol/L）
c_AES/（mmol/L）	大肠杆菌	绿脓杆菌
0	0.639	7.99
1.31	0.511	7.67
6.54	1.600	9.58
13.09	2.880	11.20
19.63	3.830	13.70
26.18	5.110	17.60
39.27	7.990	20.40
52.36	10.200	25.60
104.71	17.900	38.30
157.07	25.600	57.50
209.42	35.100	76.70
261.78	47.900	95.80

c_AEO9/（mmol/L）	MIC/（mmol/L）
c_AEO9/（mmol/L）	金黄色葡萄球菌	大肠杆菌	绿脓杆菌
0	0.511	0.639	7.99
0.32	0.256	0.383	5.11
1.61	0.319	0.383	4.47
3.22	0.767	5.750	15.30
8.05	1.530	10.200	19.80
16.10	3.190	12.800	25.60
24.15	3.830	15.300	33.20
32.21	5.110	19.200	42.20
64.41	9.580	31.900	63.90
96.62	11.500	51.100	95.80
128.82	15.300	63.900	121.00
161.03	20.400	76.700	141.00

[1]	Yuhuan Yan,Hechao Pan,Hongjie Bao,Jianhong Zhao,Yibo He. Application properties of a polymeric surfactant in different detergents [J]. China Surfactant Detergent & Cosmetics, 2024, 54(8): 930-938.
[2]	Jitao Liu,Yuxi Wang,Xuanlin Yang. Study on the surface activity of Panax notoginseng root extract and the properties of the mixed system [J]. China Surfactant Detergent & Cosmetics, 2024, 54(8): 974-980.
[3]	Binlin Pan. Study on the performance of extended surfactants in reducing interfacial tension under seawater conditions [J]. China Surfactant Detergent & Cosmetics, 2024, 54(7): 759-766.
[4]	Limei Sun, Haifeng He, Shenfa An, Zhiyong Luan, Peng Sun, Yang Wang, Feng Yan. Study on the emulsion-breaking performance of cross-linked polyether demulsifiers for the emulsion produced by surfactant/polymer flooding in Shengli oilfield [J]. China Surfactant Detergent & Cosmetics, 2024, 54(7): 795-802.
[5]	Zidi Wang, Cheng Zhou, Huaming He, Qian Jiao, Qianqian Su, Yan Jia. Advances in the effects of shampoo surfactants on scalp and their underlying mechanisms [J]. China Surfactant Detergent & Cosmetics, 2024, 54(6): 733-743.
[6]	Pengju Chu, Xiaoling Yu, Keqi Hou, Wenhui Wang, Longjie Li, Jijiang Ge. Study on the regulatory effect of Mn²⁺ ions on the formation of worm-like micelles of α-olefin sulfonate/lauramidopropyl hydroxysulfobetaine [J]. China Surfactant Detergent & Cosmetics, 2024, 54(6): 621-629.
[7]	Xuzhao Yang, Kangkang Yuan, Longhuan Wu, Caiqi Niu, Yingying Zhang, Yakun Li, Jun Wang. Application of surfactants in the preparation of MOFs [J]. China Surfactant Detergent & Cosmetics, 2024, 54(5): 581-587.
[8]	Zilong Liu, Yanxiao Hei, Di Shi, Yufei Xiao, Xue Li. Recent advances of surfactants and their adsorption characteristics in oil recovery [J]. China Surfactant Detergent & Cosmetics, 2024, 54(4): 457-466.
[9]	Yifan He, Wenhai Wu, Munan Su, Xiaolong Jiang, Yuhong Liu. In vivo molecular mechanism of surfactant-induced skin irritation and skin protection by confocal Raman spectroscopy [J]. China Surfactant Detergent & Cosmetics, 2024, 54(4): 401-409.
[10]	Zhisheng Zhang, Chanliang Shen, Jianxun Li, Yanqiang Liu, Weiwei Han, Sanbao Dong. Preparation and performance of betaine/AOS/Gemini ternary surfactant foam for gas well deliquification [J]. China Surfactant Detergent & Cosmetics, 2024, 54(3): 239-249.
[11]	Guofeng Li, Kainan Liu, Wenlong Mo, Teng Ma. Performance evaluation of a system of imbibition oil displacement agent in shale reservoir [J]. China Surfactant Detergent & Cosmetics, 2024, 54(3): 250-258.
[12]	Hongmei Zhang, Yongmin Zhang. Synthesis and properties of a choline-fatty-acid-based ionic liquid surfactant [J]. China Surfactant Detergent & Cosmetics, 2024, 54(2): 149-155.
[13]	Xinzhi Liang, Ting Sun, Xinyao Ye, Yukai Wang, Liang Bai, Hongguang Li. Study on the mixed systems of polyether organosilicon surfactant and classic anionic surfactants [J]. China Surfactant Detergent & Cosmetics, 2024, 54(10): 1155-1165.
[14]	Mengying Yuan, Dehua Wang, Huikai Nan, Tao Geng, Hongyi Liu, Hailin Zhu. Corrosion-inhibiting effects of triazinyl Gemini surfactants on carbon steel in aqueous solution containing 0.5 mol/L H₂SO₄ [J]. China Surfactant Detergent & Cosmetics, 2024, 54(10): 1145-1154.
[15]	Ziwei Zhang, Yongli Yan, Bingcheng He, Longlong Dou, Song Shi. Experimental study of non-aqueous foams stabilized with different surfactant aggregates [J]. China Surfactant Detergent & Cosmetics, 2024, 54(10): 1181-1190.