4种原料在高渗脱水细胞模型中的作用研究

doi:10.3969/j.issn.2097-2806.2024.10.009

Abstract

Abstract:

It aims to investigate the protective effects of sodium hyaluronate, panthenol, Portulaca oleracea L. and Calendula officinalis L. on hyperosmotic dehydration-induced injury of human immortalized keratinocytes （HaCaT）. The safety mass concentrations of four raw materials were screened by detecting cell viability, and the secretion of hyaluronic acid （HA） was determined using the ELISA method. The expression of HaCaT barrier function related genes （OVOL1, EREG, TGM1, TGM2, IVL, IRF6, THBS1, CASP14） was detected at the mRNA level to explore the regulatory effect of four raw materials on these genes. The results demonstrate that pretreatment with the four kinds of raw materials could increase the cell viability after hyperosmotic dehydration, promote the secretion of HA, and improve the expression of barrier function related genes after hyperosmotic dehydration, among which panthenol and Calendula officinalis L. are better. The results show that the four raw materials have a certain protective effect on the hyperosmotic dehydration cell model, which provides data support for its application in cosmetics.

Key words: hypertonic dehydration cell model, protective effect, barrier repair, efficacy evaluation of cosmetics, soothing ingredients

CLC Number:

TQ658

Yuanyuan Gao, Qidan Tan, Chencan Cao, Haifeng Chen, Yuying Jia, Lei Liu. Study on the effect of four kinds of raw materials in hypertonic dehydration cell model[J].China Surfactant Detergent & Cosmetics, 2024, 54(10): 1218-1226.

Figures/Tables 8

Tab. 1

Tab. 2

Fig. 1

Fig. 2

Fig. 3

Fig. 4

Fig. 5

Fig. 6

References 16

[1]	Kim Y R, Cheon H I, Misery L, et al. Sensitive skin in Korean population: an epidemiological approach[J]. Skin Research and Technology, 2018, 24 (2) : 229-234. doi: 10.1111/srt.12418 pmid: 29067709
[2]	Misery L, Ständer S, Szepietowski J, et al. Definition of sensitive skin: an expert position paper from the special interest group on sensitive skin of the international forum for the study of itch[J]. Acta Dermato Venereologica, 2017, 97 (1) : 4-6.
[3]	Li A, Yang Y Y. Research progress of causes and pathogenesis of sensitive skin[J]. Detergent & Cosmetics, 2022, 45 (10) : 61-66.
[4]	Misery L. Sensitive skins may be neuropathic disorders: lessons from studies on skin and other organs[J]. Cosmetics, 2021, 8 (1): 14.
[5]	Kern C, Gombert C, Roso A, et al. Soothing effect of virgin coriander seed oil on sensitive skin[J]. Ocl-Oilseeds and Fats Crops and Lipids, 2020, 27: 49.
[6]	Meng L L, Du T, Wang X X, et al. Study on the application of hyaluronic acid in cosmetics[J]. Shandong Chemical Industry, 2018, 47 (18) : 52-54, 56.
[7]	Liang H, Zhang T M, He Y L. Application of sodium hyaluronate in cosmetology field[J]. Chinese Journal of Biochemical and Pharmaceuticals, 2003, 24 (3) : 158-159.
[8]	Wang W J, Chen X Y, Zhong W B, et al. Determination of D-pantothenol in cosmetics by high performance liquid chromatography[J]. Guangdong Chemical Industry, 2023, 50 (18) : 185-187.
[9]	Wang J, Yuan M J, Zhang H. Advances in chemical constituents and pharmacological effects of Portulaca oleracea[J]. Chinese Wild Plant Resources, 2023, 42 (5) : 74-82.
[10]	Zhang H Y, Wang P, Li Y M, et al. Research progress of Portulaca oleracea in cosmetics[J]. Detergent & Cosmetics, 2022, 45 (12) : 47-50.
[11]	Chen L Y, Gao N, Gong Y M, et al. Preparation of a kind of Calendula liposome gel[J]. Guangdong Chemical Industry, 2018, 45 (15) : 85-86.
[12]	Qu W J. Hyaluronic acid, HA application in skin care[J]. China Cosmetics Review, 2020 (8) : 94-97.
[13]	Lu T F. Patent technology review on preparation, purification and application of hyaluronic acid[J]. Guangdong Chemical Industry, 2021, 48 (15) : 110-111.
[14]	Lin Z Q, Du W G, Li D. Research prospect of hyaluronic acid derivatives[J]. Yunnan Chemical Technology, 2021, 48 (11) : 16-17.
[15]	Mitoma C, Nakahara T, Uchi H, et al. Preferential expression of ovol1 in inner root sheath of hair, sebaceous gland, eccrine duct and their neoplasms in human skin[J]. Fukuoka Igaku Zasshi, 2014, 105 (8): 166.
[16]	Hu Y W, Gong T, Liu C. Study on the moisturizing and barrier promoting effects of rose/iris/lily active composite based on 3D baby skin model[J]. Detergent & Cosmetics, 2024, 47 (1) : 41-47.

No.	Gene name	Primer sequence
1	OVOL1	Forward: 5’-GCCACATGAAGTGTCACAACGA-3’
1	OVOL1	Reverse: 5’-ACTCCTCACACACGTACAGCTTG-3’
2	EREG	Forward: 5’-TTTCCATCTTCTACAGGCAGTCC-3’
2	EREG	Reverse: 5’-CATCGGACACCAGTATAACCCAC-3’
3	IVL	Forward: 5’-GTTCCTCCTCCAGTCAATACCCA-3’
3	IVL	Reverse: 5’-CACATTCTTGCTCAGGCAGTCC-3’
4	THBS1	Forward: 5’-GAGAATGCTGTCCTCGCTGTT-3’
4	THBS1	Reverse: 5’-CTCGTAGAACAGGAGGTCCACTC-3’
5	TGM1	Forward: 5’-ATGTCTCAGGCCACGTCAAG-3’
5	TGM1	Reverse: 5’-CCGGAAGACGACATTGGTGA-3’
6	TGM2	Forward: 5’-CTCACCCAGCAGGGCTTTAT-3’
6	TGM2	Reverse: 5’-CCATCTTCAAACTGCCCAAAA-3’
7	IRF6	Forward: 5’-AGATGGAAGTACCCCAGGCAC-3’
7	IRF6	Reverse: 5’-AGGCTGACGGGACCAAAGA-3’
8	CASP14	Forward: 5’-GTCAGTTGTGCCTTCGTGGTA-3’
8	CASP14	Reverse: 5’-GGTTTGTGGGCTGTCTTTGATG-3’

Stage 1	Stage 2 （40 cycles）	Stage 3 （Melting curve）
95 ℃, 30 sec, predenaturation	95 ℃, 15 sec, denaturation	65 ℃→95 ℃
	60 ℃, 30 sec, annealing/extension	Collect fluorescent signal every 0.5 ℃ of temperature increase

Study on the effect of four kinds of raw materials in hypertonic dehydration cell model

RichHTML

PDF (PC)

Like

Knowledge

Abstract

Cite this article

share this article

Figures/Tables 8

References 16

Related Articles 3

Metrics

Comments

Recommended 10

[1]	Ma Xiaoyu, Yan Xiaojuan, Bao Xijun. Metal ions: the biological effects on skin and the application in cosmetics [J]. China Surfactant Detergent & Cosmetics, 2023, 53(11): 1315-1324.
[2]	Zhou Lidan,Lu Yina,Liu Qing. Mechanism of Citrus reticulata extract in relieving skin sensitivity [J]. China Surfactant Detergent & Cosmetics, 2022, 52(4): 418-424.
[3]	Fan Ting,Zhao Jianfeng,Chang Yejun,Ji Le. Effect of recombinant humanized type Ⅲ collagen on expression of skin functional related genes [J]. China Surfactant Detergent & Cosmetics, 2022, 52(12): 1326-1332.