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日用化学工业（中英文）

日用化学工业 ›› 2015, Vol. 45 ›› Issue (6): 354-356.doi: 10.13218/j.cnki.csdc.2015.06.012

• 分析与检测 • 上一篇    

高效液相色谱法测定化妆品中的二羟基丙酮

李莉, 曹进, 张庆生   

  1. 中国食品药品检定研究院,北京 100050
  • 收稿日期:2015-01-19 出版日期:2015-06-22 发布日期:2019-04-01
  • 通讯作者: 曹进,研究员,博士,电话:(010)67095929,E-mail:cao1208@gmail.com。
  • 作者简介:李 莉(1981-),女,山东人,助理研究员,硕士,电话:(010)67095929,E-mail:lili_nicpbp@126.com。
  • 基金资助:
    国家食品药品监督管理总局资助项目(201215)

Determination of dihydroxyacetone content in cosmetics by high performance liquid chromatography

LI Li, CAO Jin, ZHANG Qing-sheng   

  1. National Institute of Food and Drug Control,Beijing 100050,China
  • Received:2015-01-19 Online:2015-06-22 Published:2019-04-01

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摘要： 建立了测定化妆品中二羟基丙酮的高效液相色谱检测方法。采用资生堂PC HILIC色谱柱(4.6 mm×250 mm,5 μm),流动相V(乙腈)∶V(5 mmol/L乙酸铵溶液)=92∶8,流速1.0 mL/min,检测波长272 nm,乙腈(体积分数为80%)为溶剂,超声提取样品。结果表明,在此条件下,二羟基丙酮在0.1～1.0 g/L内线性关系良好(r=0.999 8),回收率为92.7%~99.9%,相对标准偏差(RSD)为0.55%~1.96%。该方法快捷、简便、准确,可用于测定化妆品中的二羟基丙酮。

关键词: 化妆品, 二羟基丙酮, 高效液相色谱

Abstract: A method for determination the content of dihydroxyacetone in cosmetics was established.A Shiseido PC HILIC column (4.6 mm×250 mm,5 μm) was used with detection wavelength 272 nm.The mobile phase is composed of V(acetonitrile)∶V(5 mmol/L ammonium acetate solution)=92∶8,at a flow rate 1.0 mL/min.Samples were extracted under assistance of ultrasonic wave with 80% acetonitrile as the solvent.Results showed that when the mass concentration of dihydroxyacetone is in the range of 0.1~1.0 g/L,the relationship between mass concentration and the corresponding peak area shows good linearity (r=0.999 8).The recovery is 92.7%~99.9%,and RSD lies within 0.55%~1.96%.The method is simple and accurate.

Key words: cosmetics, dihydroxyacetone, high performance liquid chromatography

中图分类号: 

  • TQ658.5+9