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日用化学工业（中英文）

日用化学工业 ›› 2015, Vol. 45 ›› Issue (1): 55-59.doi: 10.13218/j.cnki.csdc.2015.01.013

• 分析与检测 • 上一篇    

正相高效液相色谱法同时测定化妆品中2种熊果苷含量

张乐1, 王卫华2, 姜艳艳1, 杨元1, 刘斌1   

  1. 1.北京中医药大学 中药学院,北京 100102;
    2.中国计量科学研究院,北京 100013
  • 出版日期:2015-01-22 发布日期:2019-04-01
  • 通讯作者: 刘 斌,电话:13501398172,E-mail:liubinyn67@163.com。
  • 作者简介:张乐(1988-),女,硕士研究生,电话:15652779321,E-mail:gail3123@sina.com。
       
  • 基金资助:
    国家质检总局公益行业专项资助项目(2012104001);北京中医药大学创新团队资助项目(2011-CXTD-12)

Simultaneous determination of α-,β-arbutin in cosmetics by normal phase high performance liquid chromatography

ZHANG Le1, WANG Wei-hua2, JIANG Yan-yan1, YANG Yuan1, LIU Bin1   

  1. 1.School of Chinese Pharmacy,Beijing University of Chinese Medicine,Beijing 100102,China;
    2.National Institute of Metrology P. R. China,Beijing 100013,China
  • Online:2015-01-22 Published:2019-04-01

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摘要： 采用正相高效液相色谱,建立了化妆品中α-,β-熊果苷的含量测定方法。采用S-Chiral A色谱柱(250 mm×4.6 mm,5 μm)和二极管阵列检测器,以V(正己烷)∶V(乙醇)=70∶30的混合溶液为流动相,流速为0.8 mL/min,检测波长为226 nm,柱温为30 ℃。α-,β-熊果苷的检出限(S/N=3)分别为1.5和3.6 ng,定量限(S/N=10)分别为1.6和6.3 ng,10~500 mg/L内,峰面积与质量浓度呈良好的线性关系,相关系数分别为1.000 0和0.999 0。高、中、低不同水平的加标回收率为99.61%~103.36%,RSD不大于0.80% (n=6)。该方法操作简便,准确度、灵敏度和稳定性高,可用于化妆品中α-,β-熊果苷的含量测定。

关键词: 化妆品, 正相高效液相色谱法, α-熊果苷, β-熊果苷, 测定

Abstract: An analysis method was established for determination of α-,β-arbutin in cosmetics by normal phase high performance liquid chromatography. The separation was performed on an S-Chiral A column (250 mm×4.6 mm,5 μm) with n-hexane-ethanol (volume ratio 70∶30) as the mobile phase at a flow rate of 0.8 mL/min. The detection wavelength was 226 nm. Operating temperature was 30 ℃. The limit of detection of α-,β-arbutin are 1.5 and 3.6 ng respectively,and the limit of quantification are 1.6 and 6.3 ng respectively. A good linear relationship is obtained between the peak area and the mass concentration of α-,β-arbutin in the range of 10~500 mg/L and the correlation coefficient are 1.000 0 and 0.999 0 respectively. The recoveries of α-,β-arbutin at different spiked level are 99.61%~103.36% with the relative standard deviations (RSD) no more than 0.80%.

Key words: cosmetics, normal phase high performance liquid chromatography, α-arbutin, β-arbutin, determination

中图分类号: 

  • TQ658.2