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日用化学工业（中英文）

日用化学工业 ›› 2015, Vol. 45 ›› Issue (1): 49-54.doi: 10.13218/j.cnki.csdc.2015.01.012

• 分析与检测 • 上一篇    下一篇

高效液相色谱法测定染发剂中32种禁限用染料

严巍1, 胡磊2, 周明昊1,2   

  1. 1.浙江工业大学 化学工程学院,浙江 杭州 310014;
    2.浙江省食品药品检验研究院,浙江 杭州 310004
  • 发布日期:2019-04-01
  • 通讯作者: 周明昊,硕士,主任药师,电话:(0571)86458914。
  • 作者简介:严巍(1989-),男,浙江人,硕士研究生,E-mail:1017373444@qq.com。
       

Determination of 32 kinds of banned and/or restricted dyestuff in hair dyes by high performance liquid chromatography

YAN Wei1, HU Lei2, ZHOU Ming-hao1,2   

  1. 1.College of Chemical Engineering,Zhejiang University of Technology,Hangzhou,Zhejiang 310014,China;
    2.Zhejiang Institute for Food and Drug Control,Hangzhou,Zhejiang 310004,China
  • Published:2019-04-01

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摘要： 建立了染发剂中32种禁限用染料含量的反相高效液相色谱测定方法。根据每个化合物的pKa将32种物质分成3组,采用ZORBAX Extend C18 (4.6 mm×250 mm,5 μm)和ZORBAX Bonus-RP C18 (4.6 mm×250 mm,5 μm)色谱柱,以甲醇、乙腈和pH分别为7.0,8.0和9.0的缓冲盐溶液作为流动相,在流速为1.0 mL/min,检测波长分别为280,230和250 nm的条件下进行测定。结果表明,相对标准偏差均小于5%,加标回收率为70%~130%。

关键词: 染发剂, 高效液相色谱法, 染料

Abstract: A reversed phase high performance liquid chromatography (HPLC) method was established for the determination of 32 kinds of banned and/or restricted dyestuff in hair dyes.The 32 kinds of dyestuff were divided into three groups according to the value of pKa of each component.ZORBAX Extend C18 (4.6 mm×250 mm,5 μm) column and ZORBAX Bonus-RP C18 (4.6 mm×250 mm,5 μm) column were used.Methanol,acetonitrile and buffer salts with pH of 7.0,8.0 and 9.0 respectively were used as the mobile phase.The flow rate was 1.0 mL/min and the detection wavelengths were 280,230 and 250 nm respectively.All the RSD values are lower than 5%.The spiked recovery achieves 70% to 130%.

Key words: hair dye, high performance liquid chromatography, dyestuff

中图分类号: 

  • TQ658.3+4