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日用化学工业 ›› 2018, Vol. 48 ›› Issue (2): 99-102.doi: 10.13218/j.cnki.csdc.2018.02.008

• 开发与应用 • 上一篇    下一篇

蛇皮胶原蛋白的提取工艺及优化

丁瑞敏1,2, 周华锋2,3, 李赟高3, 蔡衡3, 段明星2, 靳清贤1   

  1. 1.郑州轻工业学院 材料与化学工程学院,河南 郑州 450001;
    2.清华大学 生命科学学院,北京 100084;
    3.江苏隆力奇生物科技股份有限公司,江苏 常熟 215500
  • 收稿日期:2017-09-15 发布日期:2019-03-21
  • 通讯作者: 周华锋,高级工程师,电话:13426015613,E-mail:zhf031689@163.com。靳清贤,讲师,电话:18203684694,E-mail:13253525730@163.com。
  • 作者简介:丁瑞敏(1992-),女,河南开封人,硕士研究生,电话:18801065578,E-mail:dingrm0422@126.com。

Snake skin collagen extraction process and its optimization

DING Rui-min1,2, ZHOU Hua-feng2,3, LI Yun-gao3, CAI Heng3, DUAN Ming-xing2, JIN Qing-xian1   

  1. 1.Department of Material and Chemical Engineering,Zhengzhou University of Light Industry,Zhengzhou,Henan 450001,China;
    2.School of Life Sciences,Tsinghua University,Beijing 100084,China;
    3.Jiangsu Longliqi Biotechnology Limited Company,Changshu,Jiangsu 215500,China
  • Received:2017-09-15 Published:2019-03-21

摘要: 以蛇皮为原料,以胶原蛋白提取率为指标,采用高温酸法提取胶原蛋白。研究了有机酸种类及柠檬酸浓度、提取温度、浸提时间和固液比5个因素对蛇皮胶原蛋白提取率的影响。以单因素试验为基础,通过正交试验优化了蛇皮胶原蛋白的提取工艺。确定酸法提取蛇皮中胶原蛋白的较佳工艺为:以1 mol/L的柠檬酸作为浸提剂,采用1∶60 (g∶mL)的固液比,在110 ℃下提取3 h,此时提取率较高,为87.01%。

关键词: 化妆品原料, 蛇皮, 胶原蛋白, 提取, 柠檬酸

Abstract: Taking snake skin as raw material,and the extraction rate of collagen as the index,the high temperature acid method was used to extract collagen.The influences of the organic acid types and the concentration of citric acid,extraction temperature,extraction time and solid-to-liquid ratio on the extraction rate of collagen were studied.Based on single factor experiment,the extraction process of collagen of snake skin was optimized by orthogonal test.The optimized extraction process was obtained by acid extraction method which is found at the conditions of 1 mol/L initial concentration of citric acid as extraction agent with the solid-to-liquid ratio of 1∶60 (g∶mL),at a temperature of 110 ℃ of 3 h,the extraction rate is 87.01%.

Key words: cosmetic ingredient, snake skin, collagen, extraction, citric acid

中图分类号: 

  • TQ658