关键词: 多肽, 抗衰老, D-半乳糖, 抗炎, 抗氧化, 细胞外基质, 细胞增殖

Abstract:

Delaying skin aging has always been a hot topic in cosmetic research. In recent years, it has been reported that active peptides have the effect of delaying skin aging, thus research on substances that delaying skin aging is of great significance to the development of cosmetics and the application in the field of skin treatment. In the present study, we evaluated the anti-aging abilities of five bioactive peptides and the enhancing effect of the poly-peptide composition containing five bioactive peptides by four cell experiments and Elisa assay of cell proliferation, anti-oxidation, anti-inflammation, and promotion of collagen. Then, UVB radiation combined with D-galactose subcutaneous injection was adopted to establish animal skin aging model to investigate the anti-aging effects of the poly-peptide composition. Our data demonstrates that the five bioactive peptides have certain ability to promote cell proliferation, anti-oxidation, anti-inflammatory and promote collagen synthesis, and the poly-peptide composition have a significant enhancing effect in vitro. Importantly, after the administration of poly-peptide composition in vivo, the epidermal thickness decreases and tends to restore to normal （P<0.05）, and the contents of collagen and elastin increase, respectively （P<0.05）. Enzyme-linked immunosorbent assay results show that after the administration of poly-peptide composition, the expression of IL-1, IL-6, and TNF-α are down-regulated （P<0.05）, the expression of MMP3 and MMP9 is inhibited （P<0.05）, and the increased expression of β-galactosidase is significantly reduced （P<0.05）. The above results suggest that the composition of five bioactive peptide can play an anti-aging effect on the skin caused by UVB radiation combined with D-galactose subcutaneous injection.

Key words: bioactive peptide, anti-aging, D-galactose, anti-inflammatory, antioxidant, extracellular matrix, cell proliferation