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日用化学工业(中英文) ›› 2022, Vol. 52 ›› Issue (12): 1353-1358.doi: 10.3969/j.issn.1001-1803.2022.12.014

• 分析与检测 • 上一篇    下一篇

气相色谱法测定化妆品中角鲨烷含量

郭春景*(),蒋云杰,程恒光,李晓霞,蒋晴,吴凯宾   

  1. 杭州千岛湖天鑫有限公司,浙江 杭州 310000
  • 收稿日期:2022-03-24 修回日期:2022-12-07 出版日期:2022-12-22 发布日期:2022-12-29
  • 通讯作者: 郭春景

Determination of squalane in cosmetics by gas chromatography

Guo Chunjing*(),Jiang Yunjie,Cheng Hengguang,Li Xiaoxia,Jiang Qing,Wu Kaibin   

  1. Hangzhou Qiandaohu Tianxin Co., LTD., Hangzhou, Zhejiang 310000, China
  • Received:2022-03-24 Revised:2022-12-07 Online:2022-12-22 Published:2022-12-29
  • Contact: Chunjing Guo

摘要:

建立了气相色谱法测定化妆品中角鲨烷含量的研究方法,优化样品前处理和仪器条件参数,对水溶性化妆品用石油醚萃取,脂溶性化妆品经氢氧化钾-乙醇溶液进行皂化,用正己烷萃取,萃取液经过氮吹浓缩,用正己烷复溶定容,利用HP-5色谱柱和程序升温的柱温箱进行分离,FID检测器检测,外标法定量分析。结果表明:角鲨烷在1~ 1 000 μg/mL的质量浓度范围内呈现良好的线性关系,线性相关系数R2等于1,检出限、定量限分别为1和3 mg/kg,低、中、高3个含量水平下的加标回收率范围为92.7%~103.5%,相对标准偏差范围为1.83%~5.39%。该方法不仅实验操作简单、仪器的重复性好,而且精密度好、灵敏度高、准确度可靠,具有很好的实用性,能够准确测定化妆品中角鲨烷的 含量。

关键词: 气相色谱法, 化妆品, 角鲨烷

Abstract:

A gas chromatographic method was established for determination of squalane in cosmetics. The GC instrument conditions and sample pretreatment process were optimized. The water-soluble cosmetics were extracted with petroleum ether, while the fat-soluble cosmetics were saponified with potassium hydroxide-ethanol solution and then extracted by n-hexane. The extraction liquid was concentrated by nitrogen-blowing, redissolved with n-hexane and brought to volume with n-hexane. The sample was analyzed on an HP-5 column with temperature programming, detected by an FID detector, and quantitated using external standard method. The results showed that, the optimal injection volume was 1 μL, the best saponification temperature was 80 ℃, the optimal saponification time was 30 min, the optimal number of times for extraction was 3, the relative standard deviation of instrument repeatability was 1.13%, and a good linear relationship was obtained between the mass concentration range of squalane from 1 to 1 000 μg/mL with the correlation coefficient of 1.The limits of detection and quantitation were 1 mg/kg and 3 mg/kg, respectively. At low, medium and high levels, the spiked recoveries ranged from 92.7% to 103.5%, and the range of relative standard deviation was 1.83%-5.39%. This method is simple in experimental operation, and has good repeatability, good precision, high sensitivity and reliable accuracy. It can accurately determine the content of squalane in cosmetics.

Key words: gas chromatography, cosmetics, squalane

中图分类号: 

  • TQ658