葡萄籽发酵液抗衰老及美白功效研究

doi:10.3969/j.issn.1001-1803.2022.05.013

摘要/Abstract

摘要：

利用微生物发酵技术,将葡萄籽以红酒酵母发酵得到葡萄籽发酵液,选用抗衰模式生物秀丽隐杆线虫,建立小鼠黑素瘤细胞美白模型,从细胞水平与分子水平层面探究葡萄籽发酵液在抗衰老和美白方面的功效作用,并对葡萄籽发酵液进行安全性评定。研究结果显示葡萄籽发酵液能够有效抵御衰老并抑制黑色素生成,具有抗衰、美白效果以及良好的安全性,具备成为化妆品功效原料的应用潜力。

关键词: 葡萄籽, 发酵技术, 秀丽隐杆线虫, 抗衰老, 美白, 安全性评价

Abstract:

As the largest by-products of the grape processing industry, the development and utilization of grape seeds has become one of the urgent problems to be solved. Grape seeds are rich in active substances, which have the functions of anti-oxidation, anti-aging, anti-radiation and whitening. They can remove excessive oxygen free radicals produced by the body under oxidative stress conditions, and reduce the skin injury caused by external radiation and environmental pollution, which have a potential role in skin care. In this study, grape seeds were fermented using microbial fermentation technology with red wine yeast to obtain grape seed fermentation broth. The anti-aging model organism C.elegans was selected, and a mouse melanoma cell whitening model was established to explore the anti-aging and whitening effects of grape seeds from the cellular and molecular levels. Research results show that grape seed fermentation broth can effectively resist aging and inhibit melanin production, which has anti-aging and whitening effects. It also has good safety, and has the application potential as cosmetic functional raw materials. The by-products of grape processing have been reused, and its potential application value has been discovered, which is in line with the concept of green development.

Key words: grape seed, fermentation technology, C.elegans, anti-aging, whitening, safety evaluation

中图分类号:

TQ658

王冬冬,孙倩茹,王子文,方嘉璇,李萌,王昌涛. 葡萄籽发酵液抗衰老及美白功效研究[J]. 日用化学工业, 2022, 52(5): 545-552.

Wang Dongdong,Sun Qianru,Wang Ziwen,Fang Jiaxuan,Li Meng,Wang Changtao. Study on the anti-aging and whitening effects of grape seed fermentation broth[J]. China Surfactant Detergent & Cosmetics, 2022, 52(5): 545-552.

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基因	方向	引物
β-actin	F	CTG AAG CCC CAC TCA ATC CA
β-actin	R	GCC AAG TCA AGA CGG AGG AT
daf-2	F	GTA ATT GGA GGC CGT TCG CT
daf-2	R	CGT GGG CAC ATC AAT CCA GT
daf-16	F	GTA ATT GGA GGC CGT TCG CT
daf-16	R	CGT GGG CAC ATC AAT CCA GT
hsp-16.2	F	CGT CGA AGA GAA ATC TGC TGA A
hsp-16.2	R	TGC AGC GAA CAA TAC TGT AAT TTA TG
sod-3	F	GAC GAT CAA CCC CTG TCG AA
sod-3	R	TAC TGT TCT TCG GGG AAC GC

试剂	体积/μL
2×SuperReal PreMix Plus	10.0
F	0.3
R	0.3
50×ROX Reference Dye	0.25
cDNA	1.0
RNase-free ddH₂O	8.15

评分/分	分级	出血现象	凝血现象	出血现象
0	无	—	—	—
1	轻度	细小血管出血和少量出血	血管内（外）轻度凝血或CAM膜轻度浑浊	仅小血管融解
2	中度	小血管和大血管出血,并有明显量的血液流出	血管内（外）中度凝血或CAM膜中度浑浊	小血管和大血管融解
3	重度	几乎所有血管都出血,大量血液流出	血管内（外）重度凝血或CAM膜重度浑浊	大血管和全部血管树都融解

评分范围/分	刺激性分类
ES<6	无刺激性
6≤ES<12	轻度刺激性
12≤ES<15	中度刺激性
ES≥15	重度刺激性

ρ/（μg/mL）	线虫条数/条	平均寿命/d	最大寿命/d
对照组	150	19.24±0.96	26.31±1.32
1	150	19.88±0.99	30.43±1.52^*
4	150	20.24±1.01	28.72±1.44
8	150	21.64±1.08^*	32.28±1.61^**