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日用化学工业 ›› 2021, Vol. 51 ›› Issue (9): 918-924.doi: 10.3969/j.issn.1001-1803.2021.09.016

• 分析与检测 • 上一篇    

QuEChERS-UPLC法测定化妆品中10种性激素

胡贝1,2,*(),李丽霞1,2,刘红1,2,黄伟1,2,丁晓萍1,2   

  1. 1.湖北省药品监督检验研究院,湖北 武汉 430075
    2.湖北省药品质量检测与控制工程技术研究中心,湖北 武汉 430075
  • 收稿日期:2020-12-25 修回日期:2021-08-25 出版日期:2021-09-22 发布日期:2021-09-23
  • 通讯作者: 胡贝

Determination of 10 sex hormones in cosmetics by QuEChERS-UPLC

Hu Bei1,2,*(),Li Lixia1,2,Liu Hong1,2,Huang Wei1,2,Ding Xiaoping1,2   

  1. 1. Hubei Institute for Drug Control, Wuhan, Hubei 430075, China
    2. Hubei Engineering Research Center for Drug Quality Control, Wuhan, Hubei 430075, China
  • Received:2020-12-25 Revised:2021-08-25 Online:2021-09-22 Published:2021-09-23
  • Contact: Bei Hu

摘要:

建立了QuEChERS-UPLC法测定化妆品中10种性激素的方法。样品利用饱和氯化钠进行分散,乙腈超声提取后经QuEChERS净化。采用CORTECS C18+(2.1 mm×100 mm,2.7 μm)色谱柱分离,以乙腈-水为流动相进行梯度洗脱,PDA检测器多波长检测,外标法定量。结果表明,10种性激素在各自的质量浓度范围内线性关系良好(相关系数r>0.99),检出限(LOD)和定量下限(LOQ)分别为0.02~0.1 μg/mL和0.05~0.3 μg/mL。在5,25和50 μg/g 3个加标水平下的回收率为81.2%~103.5%,相对标准偏差(RSD,n=6)为0.5%~12.0%。该方法操作简便、重现性好,适用于化妆品中性激素的测定。

关键词: QuEChERS, 超高效液相色谱法, 化妆品, 性激素, 测定

Abstract:

A method of QuEChERS coupled with ultra-high performance liquid chromatography was developed for determination of 10 sex hormones in cosmetics. The sample was dispersed using saturated sodium chloride, and purified by QuEChERS after acetonitrile ultrasound extraction. Chromatographic separation was achieved on a CORTECS C18+ column (2.1 mm×100 mm, 2.7 μm) by gradient elution using acetonitrile-water as mobile phase. PDA detector was used to test samples at multiple wavelengths, and external standard method was used for quantification. Results show that there is a good linear relationship for each of the 10 sex hormones in respective mass concentration range with their correlation coefficients (r) all above 0.99. The limits of detection (LOD) and the limits of quantitation(LOQ) for the 10 sex hormones are in the range of 0.02-0.1 μg/mL and 0.05-0.3 μg/mL, respectively. Average recoveries at three levels of 5, 25 and 50 μg/g are in the range of 81.2%-103.5%, with relative standard deviations (RSD, n=6) of 0.5%-12.0%. With the advantages of simplicity and good reproducibility, this method is suitable for the analysis of sex hormones in cosmetics.

Key words: QuEChERS, ultra-high performance liquid chromatography, cosmetics, sex hormone, determination

中图分类号: 

  • TQ658