不同比例荆芥-连翘药对挥发油成分及抗痤疮致病菌研究

doi:10.3969/j.issn.1001-1803.2021.06.003

摘要/Abstract

摘要：

采用GC-MS测定不同质量比（1:1、1:2.5、2.5:1）荆芥-连翘药对及其单味饮片挥发油的化学成分,结合抗痤疮丙酸杆菌、金黄色葡萄球菌和表皮葡萄球菌的作用,以探究荆芥-连翘药对抗痤疮致病菌的配伍意义。结果表明,荆芥挥发油主要含有胡薄荷酮（41.52%）和异薄荷酮（41.62%）,连翘挥发油主要含有β-蒎烯（38.93%）、蒎烯（19.46%）和桧烯（15.06%）;荆芥-连翘1:1配伍挥发油成分变化较少,1:2.5和2.5:1配伍挥发油成分变化较大,荆芥挥发油成分容易受连翘的影响。荆芥-连翘1:1配伍对痤疮丙酸杆菌和金黄色葡萄球菌的抑制作用较好,对表皮葡萄球菌有一定程度抑制作用,1:2.5和2.5:1配伍挥发油则表现出弱抑菌性,可能与配伍后化学成分发生变化有关。

关键词: 药对, 荆芥, 连翘, 挥发油, 痤疮, 化妆品

Abstract:

In order to explore the compatibility of Schizonepetae Herba-Forsythiae Fructus in the treatment of acne, the components of different quality proportions （1:1, 1:2.5, 2.5:1） of volatile oil were analyzed by gas chromatography-mass spectrometer（GC-MS） and the inhibitory effect on staphylococcus aureus, staphylococcus epidermidis and propionibacterium acnes in vitro were compared. The results show that Schizonepetae Herba volatile oil mainly contained p-Menthone（41.52%） and Menthone（41.62%）, and Forsythiae Fructus volatile oil mainly contained β-Pinene（38.93%）, Pinene（19.46%） and Sabinene（15.06%）. The components of volatile oil from Schizonepetae Herba-Forsythiae Fructus 1:1 had litter change, but 1:2.5 and 2.5:1 had must change. The components of volatile oil from Schizonepetae Herba were easily affected by Forsythiae Fructus. The volatile oil of Schizonepetae Herba-Forsythiae Fructus 1:1 had a better inhibitory effect on propionibacterium acnes and staphylococcus aureus, and had a certain inhibitory effect on staphylococcus epidermidis. The volatile oil in ratios of 1:2.5 and 2.5:1 showed weak inhibitory, which may be related to the content changes after compatibility.

Key words: pairsl, Schizonepetae Herba, Forsythiae Fructus, volatile oil, Propionibacterium acnes, cosmetic

中图分类号:

TQ658

邱思娃,陈素雯,孔淑霞,蔡延渠,吴燕红,李苑新. 不同比例荆芥-连翘药对挥发油成分及抗痤疮致病菌研究[J]. 日用化学工业, 2021, 51(6): 491-495.

QIU Si-wa,CHEN Su-wen,KONG Shu-xia,CAI Yan-qu,WU Yan-hong,LI Yuan-xin. Study on component analysis of different proportions of volatile oil from Schizonepetae Herba-Forsythiae Fructus and its anti-acne pathogenic bacterias[J]. China Surfactant Detergent & Cosmetics, 2021, 51(6): 491-495.

图/表 3

图 1

表 1

表 2

参考文献 14

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序号	化合物	CAS	分子式	相对含量/%
				荆芥	连翘	m（荆芥）:m（连翘）
				荆芥	连翘	1:1	1:2.5	2.5:1
1	蒎烯	007785-70-8	C₁₀H₁₆	—	19.46	10.55	8.41	2.53
2	桧烯	003387-41-5	C₁₀H₁₆	—	15.06	8.62	9.68	5.69
3	β-蒎烯	018172-67-3	C₁₀H₁₆	—	38.93	24.16	32.73	15.56
4	月桂烯	000123-35-3	C₁₀H₁₆	—	2.21	1.29	1.99	1.04
5	松油烯	000099-86-5	C₁₀H₁₆	—	2.04	1.02	—	—
6	4-蒈烯	029050-33-7	C₁₀H₁₆	—	—	—	1.48	—
7	右旋萜二烯	005989-27-5	C₁₀H₁₆	—	—	3.24	—	—
8	柠檬烯	000138-86-3	C₁₀H₁₆	—	—	—	3.26	3.30
9	γ-松油烯	000099-85-4	C₁₀H₁₆	—	3.28	1.79	2.55	1.39
10	萜品油烯	000586-62-9	C₁₀H₁₆	—	1.03	—	1.21	—
11	（+） -异薄荷酮	001196-31-2	C₁₀H₁₈O	—	—	—	11.82	30.25
12	异薄荷酮	000491-07-6	C₁₀H₁₈O	41.62	—	19.51	2.20	4.62
13	薄荷酮	010458-14-7	C₁₀H₁₈O	—	—	2.45	—	—
14	反式-异胡薄荷酮	029606-79-9	C₁₀H₁₆O	1.37	—	—	—	—
15	（-） -4-萜品醇	020126-76-5	C₁₀H₁₈O	—	7.18	4.80	9.08	4.99
16	α-松油醇	000098-55-5	C₁₀H₁₈O	—	1.48	—	1.85	—
17	胡薄荷酮	000089-82-7	C₁₀H₁₆O	41.52	—	12.15	6.94	19.48
18	薄荷烯酮	000491-09-8	C₁₀H₁₄O	2.04	—	0.49	—	1.00
19	β-石竹烯	000087-44-5	C₁₅H₂₄	1.12	—	0.50	—	0.71
20	大根香叶烯D	023986-74-5	C₁₅H₂₄	0.69	—	0.27	—	0.35
21	氧化石竹烯	001139-30-6	C₁₅H₂₄O	0.96	—	0.41	—	—

菌种	MBC/（mg·mL^-1）
	荆芥	连翘	m（荆芥）:m（连翘）
	荆芥	连翘	1:1	1:2.5	2.5:1
金黄色葡萄球菌	15.62	7.81	7.81	15.62	31.25
表皮葡萄球菌	7.81	3.90	15.62	15.62	31.25
痤疮丙酸杆菌	7.81	15.62	3.90	31.25	15.62

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