汤原温泉水联合甘草酸二钾抗炎舒敏功效的研究

doi:10.3969/j.issn.1001-1803.2021.05.010

摘要/Abstract

摘要：

利用角质形成细胞和3D重组人表皮模型构建炎症和过敏模型,验证汤原温泉水（HW）联合甘草酸二钾（DG）的抗炎和舒敏功效。细胞活力实验结果显示DG安全作用浓度为100 μmol/L,HW使用浓度为1%。抗炎方面,1% HW联合100 μmol/L DG对炎症因子复合物诱导活化的NF-κB信号通路抑制率为56%。在炎症因子复合物诱导的重组人表皮模型中,HW联合DG有效降低白介素1α （IL-1α）和白介素6 （IL-6）分泌水平。舒敏方面,HW联合DG能够显著降低血管内皮生长因子（VEGF）分泌水平并下调辣椒素受体（TRPV1）与环氧合酶（COX-2） mRNA表达。

关键词: 人重组表皮模型, 汤原温泉水, 甘草酸二钾, 抗炎, 舒敏

Abstract:

Keratinocytes and 3D skin models were applied to establish anti-inflammatory and anti-allergy models. Based on this model, the effects of hotspring water （HW） combined with dipotassium glycyrrhizinate （DG） were studied. The results showed that, the safe concentration of DG was below 100 μmol/L combined with 1% HW. The combination group could decrease the activity of NF-κB by 56% （NF-κB was induced by inflammatory cocktail）, and could significantly reduce the secretion of IL-1α and IL-6. The combination group also provided anti-allergy effect by reducing the secretion of VEGF and the expression levels of allergy related genes including TRPV1 and COX-2.

Key words: reconstructed human epidermis model, Yubara hotspring water, dipotassium glycyrrhizinate, anti-inflammatory, anti-allergy

中图分类号:

TQ658

乔武,贾霆涵,陆静,贺来健. 汤原温泉水联合甘草酸二钾抗炎舒敏功效的研究[J]. 日用化学工业, 2021, 51(5): 433-437.

QIAO Wu,JIA Ting-han,LU Jing,HE Lai-jian. Study of anti-inflammatory and anti-allergy effects of Yubara hotspring water combined with dipotassium glycyrrhizinate[J]. China Surfactant Detergent & Cosmetics, 2021, 51(5): 433-437.

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成分	ρ/（mg·L^-1）
硅（Si）	17.1
钠（Na）	54.2
钾（K）	1
钙（Ca）	<0.5
铝（Al）	<0.1
铬（Cr）	<0.1
铜（Cu）	<0.1
铁（Fe）	<0.1
锰（Mn）	<0.1
镁（Mg）	<0.1

基因	正向引物	反向引物
TRPV1	CTGCCCGACCATCACAGTC	CTGCGATCATAGAGCCTGAGG
COX-2	TAAGTGCGATTGTACCCGGAC	TTTGTAGCCATAGTCAGCATTGT
GAPDH	GGAGCGAGATCCCTCCAAAAT	GGCTGTTGTCATACTTCTCATGG