欢迎访问《日用化学工业(中英文)》,今天是

日用化学工业(中英文) ›› 2025, Vol. 55 ›› Issue (5): 601-610.doi: 10.3969/j.issn.2097-2806.2025.05.008

• 开发与应用 • 上一篇    下一篇

芹菜素和原花青素舒缓敏感肌肤的作用机制

魏来娇1,邓榕榕2,郑诗倩2,李珞炫1,王科证1,沈志滨1,2,*()   

  1. 1.广东药科大学 中药学院,广东 广州 511400
    2.广东植肤生物科技有限公司,广东 广州 510030
  • 收稿日期:2024-12-25 修回日期:2025-04-17 出版日期:2025-05-22 发布日期:2025-06-11
  • 基金资助:
    国家重点研发计划重点专项项目(2018YFC1707100)

Mechanism of the action of apigenin and proanthocyanidins in alleviating sensitive skin

Laijiao Wei1,Rongrong Deng2,Shiqian Zheng2,Luoxuan Li1,Kezheng Wang1,Zhibin Shen1,2,*()   

  1. 1. School of Traditional Chinese Medicine, Guangdong Pharmaceutical University, Guangzhou, Guangdong 511400, China
    2. Guangdong Botanical Beauty Care Biotechnology Co., Ltd., Guangzhou, Guangdong 510030, China
  • Received:2024-12-25 Revised:2025-04-17 Online:2025-05-22 Published:2025-06-11
  • Contact: *E-mail: szb8113@126.com.

摘要:

为研究芹菜素(API)和原花青素(PC)舒缓敏感肌肤(SS)的作用机制,用钙离子载体A23187刺激RBL-2H3细胞建立肥大细胞脱颗粒模型。通过联合实验发现,当API和PC以摩尔比4∶1和2∶1组合时拮抗组胺释放表现为拮抗作用(CI>1);当API和PC以摩尔比1∶1、1∶2和1∶4组合时拮抗组胺释放表现为协同作用(CI<1),其中API和PC以摩尔比1∶1组合时具有较佳的协同抗组胺释放作用(CI=0.70),组胺是肥大细胞脱颗粒的标志,表明API和PC以摩尔比1∶1联用时抑制肥大细胞脱颗粒的效率较佳,此时IC50值最小,与API单药使用相比,IC50下调了11.150 µmol/L;与PC单药使用相比,IC50下调了6.503 µmol/L。与阳性药丹皮酚(PA)相比,经过组合物处理后,β-hex分泌显著减少;F-actin细胞骨架重排减少;TNF-α、IL-4和MCP-1释放显著降低。对肥大细胞脱颗粒相关信号通路进行研究发现,组合物有效抑制细胞内Ca2+内流并显著抑制钙调蛋白依赖性蛋白激酶(CaMK)和磷脂酶C/蛋白激酶C(PLC/PKC)的磷酸化。综上,API和PC以摩尔比1∶1联用时具有较佳的协同拮抗组胺释放作用,能通过减少Ca2+内流并抑制Ca2+/CaMK和PLC/PKC通路激活,抑制肥大细胞脱颗粒模型活化,稳定细胞膜、调节炎症因子分泌,发挥舒缓敏感肌的作用。

关键词: 芹菜素, 原花青素, 肥大细胞, 敏感肌, 舒缓

Abstract:

To investigate the mechanisms of apigenin (API) and proanthocyanidins (PC) in soothing sensitive skin (SS), a mast cell degranulation model was established by stimulating RBL-2H3 cells with the calcium ionophore A23187. Based on the combinatorial experiments, it was found that when API and PC were combined at the molar ratios of 4∶1 and 2∶1, they exhibited the antagonistic effects on histamine release (combination index CI>1); when they are combined at the molar ratios of 1∶1, 1∶2 or 1∶4, they showed the synergistic effects on histamine release (CI<1). Among them, the combination of API and PC at a molar ratio of 1∶1 showed the better potent synergistic antihistamine release effect (CI=0.70). Histamine is a hallmark of the mast cell degranulation, consequently, the combination of API and PC at a molar ratio of 1∶1 yields the better efficiency in inhibiting the mast cell degranulation with the lowest IC50 value. Compared to the utilization of API or PC alone, the IC50 value was reduced by 11.150 and 6.503 µmol/L, respectively. Compared to the positive control paeonol (PA), the treatment with the combination significantly reduced the β-hex secretion, decreased the F-actin cytoskeleton rearrangement, and markedly suppressed the release of TNF-α, IL-4, and MCP-1. Further studies on the signaling pathways related to the mast cell degranulation indicated that the combination effectively inhibited the intracellular Ca2+ influx and significantly suppressed the phosphorylation of calmodulin-dependent protein kinase (CaMK) and phospholipase C/protein kinase C (PLC/PKC). In summary, the combination of API and PC at a molar ratio of 1∶1 exhibited the better synergistic antagonistic effect on the histamine release, inhibited the mast cell degranulation model activation by reducing Ca2+ influx and inhibiting the activation of Ca2+/CaMK and PLC/PKC pathways, stabilized the cell membranes, regulated the inflammatory factor secretion, and exerted an effect in alleviating sensitive skin.

Key words: apigenin, proanthocyanidins, mast cell, sensitive skin, soothing

中图分类号: 

  • TQ658