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日用化学工业（中英文）

日用化学工业（中英文） ›› 2023, Vol. 53 ›› Issue (3): 361-364.doi: 10.3969/j.issn.1001-1803.2023.03.016

• 分析与检测 • 上一篇    

高效凝胶过滤色谱法测定皂角米中多糖组分

王泽云1,*(),张博丽2   

  1. 1.运城学院 应用化学系，山西 运城 044000
    2.山西焦煤运城盐化集团股份有限公司，山西 运城 044000
  • 收稿日期:2022-03-29 修回日期:2023-03-03 出版日期:2023-03-22 发布日期:2023-03-22

Determination of polysaccharides in Gleditsia sinensis seed by high performance gel filtration chromatography

Wang Zeyun1,*(),Zhang Boli2   

  1. 1. Department of Applied Chemistry, Yuncheng College, Yuncheng, Shanxi 044000, China
    2. Yuncheng Salt Chemical Group Co., Ltd., Yuncheng, Shanxi 044000, China
  • Received:2022-03-29 Revised:2023-03-03 Online:2023-03-22 Published:2023-03-22
  • Contact: * Tel.: +86-18803593085, E-mail: wangzeyun00@126.com.

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摘要：

采用高效凝胶过滤色谱法（HPGFC）测定皂角米中多糖组分的含量，以水凝胶色谱柱Ultrahydrogel™ Linear 300 mm×7.8 mmid×2为分离柱；以0.1 mol/L硝酸钠为流动相；流速：0.8 mL/min；柱温：40 ℃。试验表明，多糖组分1#（Mw=2 115 637），2#（Mw=12 516），3#（Mw=418）在质量浓度0.10~5.00 g/L之间，峰面积与质量浓度呈良好的线性关系，相关系数γ为0.999 5，0.999 2，0.999 0（n=5），方法检出限为0.056，0.053，0.050 g/L，加标回收率为96.7%~102.0%，96.0%~101.6%，94.0%~103.6%，相对标准偏差为0.63%~1.81%，0.81%~1.94%，1.01%~2.03%（n=6）。该方法操作简便，具有良好的重复性和回收率，可用于皂角米中多糖组分的检测。

关键词: 皂角米, 高效凝胶过滤色谱法, 多糖

Abstract:

High performance gel filtration chromatography was used to determine the polysaccharide components in sapiens. The hydrogel column Ultra-hydrogel™ Linear 300 mm×7.8 mmid×2 was used as the separation column with the mobile phase of 0.1mol/L sodium nitrate, the flow rate of 0.8 mL/min, and the column temperature at 40 ℃. Test results show that the polysaccharide components 1# （Mw=2 115 637）, 2#（Mw=12 516）, 3#（Mw=418） are in the range of 0.10-5.00 g/L, the peak areas and mass concentrations appear good linear correlation with the correlation coefficient γ of 0.999 5, 0.999 2, and 0.999 0（n=5）, respectively. The detection limits are 0.056, 0.053, and 0.050 g/L, respectively, the recoveries of this method are 96.7%-102.0%, 96.0%-101.6%, and 94.0%-103.6%, respectively, and the relative standard deviations are 0.63%-1.81%, 0.81%-1.94%, and 1.01%-2.03%（n=6）, respectively. The method is simple, has good repeatability and recovery, and can be used for the determination of polysaccharide components in sapiens.

Key words: Gleditsia sinensis seed, high performance gel filtration chromatography, polysaccharide

中图分类号: 

  • TQ649