白梅花提取物抗氧化及美白功效评价

doi:10.3969/j.issn.1001-1803.2022.02.010

摘要/Abstract

摘要：

采用体外评价方法对白梅花提取物进行抗氧化及美白功效研究。通过测定白梅花提取物对超氧阴离子及羟自由基清除的半数抑制浓度（IC₅₀）,以及在H₂O₂诱导人皮肤成纤维细胞株（HFF-1细胞）氧化损伤模型中,考察白梅花提取物对细胞形态学、细胞存活率、丙二醛（MDA）含量、活性氧自由基（ROS）含量、超氧化物歧化酶（SOD）活力、谷胱甘肽（GSH）活力、Ⅰ型胶原蛋白（Col Ⅰ）以及基质金属蛋白酶1（MMP-1）含量的影响,评价其抗氧化功效。采用酪氨酸酶活性抑制实验以及小鼠黑色素瘤细胞株（B16-F10细胞）黑色素含量测定实验评价白梅花提取物的美白功效。结果表明白梅花提取物具有自由基清除作用,且白梅花60%醇提物、白梅花90%醇提物可明显提高HFF-1细胞活力、SOD活力、GSH活力以及Col Ⅰ含量,下调ROS、MDA及MMP-1含量,并明显降低B16-F10细胞中的黑色素含量,表现出明显的抗氧化及美白功效。

关键词: 白梅花, 自由基, 抗氧化, 黑色素, 酪氨酸酶

Abstract:

In vitro evaluation method was used to study the anti-oxidation and whitening effects of Prunus mume extract. Preliminary evaluation of its antioxidant activity was conducted by measuring the IC₅₀ of Prunus mume extract on superoxide anion and hydroxyl radical scavenging. And in the H₂O₂-induced oxidative damage model of human skin fibroblast cell line （HFF-1 cells）, the antioxidant activity of Prunus mume extract was evaluated by examining the cell morphology, cell survival rate, malondialdehyde （MDA） content, reactive oxygen radical （ROS） content, superoxide dismutase （SOD） activity, glutathione （GSH） activity, type I collagen （ColⅠ） content and matrix metalloproteinase 1 （MMP-1） content. The tyrosinase activity inhibition experiment and the melanin content determination experiment of B16-F10 cells were used to evaluate the whitening effect of the Prunus mume extract. The results show that the Prunus mume extract has free radical scavenging effect, and the 60% and 90% alcohol extract of Prunus mume can significantly increase the activity of HFF-1 cells, SOD activity, GSH activity and Col Ⅰ content, and decrease the content of ROS, MDA and MMP-1. And it can also significantly reduce the melanin content in B16-F10 cells. To sum up, the white plum extract has obvious antioxidant and whitening effects.

Key words: Prunus mume, free radical, antioxidant, melanin, tyrosinase

中图分类号:

TQ658

查雨锋,黄加文,詹易,李婷,颜宏,吴德松. 白梅花提取物抗氧化及美白功效评价[J]. 日用化学工业, 2022, 52(2): 172-179.

Zha Yufeng,Huang Jiawen,Zhan Yi,Li Ting,Yan Hong,Wu Desong. Evaluation of antioxidant and whitening efficacy of Prunus mume extract[J]. China Surfactant Detergent & Cosmetics, 2022, 52(2): 172-179.

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组别	IC₅₀/（mg/L）
组别	超氧阴离子	羟自由基
白梅花30%醇提物	376.4	268.5
白梅花60%醇提物	156.6	88.3
白梅花90%醇提物	96.7	67.5
阳性对照（维生素C）	11.9	18.4

组别	ROS/（IU/mL）	MDA含量/（nmol/mgprot）	SOD活力/（U/mgprot）	GSH活力/（μmol/mgprot）
空白对照组	2.65±0.17	1.42±0.41	29.22±0.99	85.06±3.83
模型组	3.51±0.16^#	2.89±0.36^#	15.28±2.15^#	56.60±2.18^#
白梅花30%醇提物	3.30±0.20	2.68±0.37	17.53±2.51	59.96±3.18
白梅花60%醇提物	3.09±0.18^*	2.26±0.34^*	20.15±3.16^*	67.31±4.86^*
白梅花90%醇提物	2.92±0.22^*	1.81±0.29^*	23.65±1.77^*	75.17±3.26^*