1,8-桉叶素驱蚊止痒功效的研究

doi:10.3969/j.issn.1001-1803.2021.07.006

摘要/Abstract

摘要：

为探究1,8-桉叶素驱蚊及其治疗局部皮肤过敏性瘙痒的效果,通过“Y”型嗅觉仪法测试了其在不同剂量梯度下对白纹伊蚊的驱避活性,采用4-氨基吡啶（4-AP）致小鼠瘙痒模型探讨了1,8-桉叶素（φ=5%,用橄榄油稀释）的止痒作用。结果表明,当1,8-桉叶素剂量为4.0 μL时,其在前4 min内对白纹伊蚊可达到100%的驱避率,且其对白纹伊蚊的驱避效果呈剂量依赖性增加;通过4-AP致小鼠瘙痒模型发现,与模型组相比,1,8-桉叶素组小鼠在10 min内的舔体次数显著减少,小鼠皮肤滤液中组胺与血清中白细胞介素31（IL-31）、血小板活化因子（PAF）、5-羟色胺（5-HT）含量均显著降低,病理切片显示1,8-桉叶素组小鼠皮肤未见水肿,同时炎细胞浸润减少。结论当1,8-桉叶素浓度达到一定值时,其对白纹伊蚊具有很好的驱避效果;且1,8-桉叶素具有显著的止痒效果,其发挥止痒作用与拮抗IL-31、PAF、5-HT、瘙痒介质组胺的释放有关。

关键词: 1,8-桉叶素, 驱蚊活性, 止痒作用

Abstract:

To explore the mosquito repellent effect and the therapeutic properties of allergic skin itching of 1, 8-cineole, the repellent activity of 1, 8-cineole against Aedes albopictus under different doses was studied by the Y-tube olfactory test. The antipruritic effect of 1, 8-cineole （φ=5%, prepared with olive oil） was studied in an animal model of mice skin scratching response induced by 4-aminopyridine（4-AP）. The results show that 1, 8-cineole at the dose of 4.0 μL repels 100% of A. albopictus in the initial 4 minutes and the repellent effect of 1, 8-cineole against A. albopictus increases in a dose-dependent manner. In addition, mice experiments show that the scratching frequency of mice treated with 1, 8-cineole reduces significantly within 10 minutes; and the contents of serum interleukin 31 （IL-31）, platelet activating factor （PAF）, serotonin （5-HT） and histamine in the skin filtrate of mice treated with 1, 8-cineole are significantly reduced compared with the model group. The pathological sections show that no edema in the skin of mice in the 1, 8-cineole group is observed and the infiltration of inflammatory cells is inhibited. In conclusion, when the concentration of 1, 8-cineole reaches a certain value, it shows obvious repellent effect on A. albopictus. 1, 8-cineole also has a significant antipruritic effect through the mechanism of antagonizing the release of IL-31, PAF, 5-HT, as well as the mediator of itching, histamine.

Key words: 1,8-cineole, mosquito-repellent activity, antipruritic effect

中图分类号:

TQ658

陈凡,姚雷,王文翠. 1,8-桉叶素驱蚊止痒功效的研究[J]. 日用化学工业, 2021, 51(7): 620-625.

Chen Fan,Yao Lei,Wang Wencui. Study on the mosquito-repelling and antipruritic effects of 1, 8-cineole[J]. China Surfactant Detergent & Cosmetics, 2021, 51(7): 620-625.

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剂量/ μL	不同时间驱避率/%
剂量/ μL	1 min	2 min	3 min	4 min	5 min	10 min	15 min
0.5	31.25±4.17^a	38.10±5.50^a	44.64±3.57^a	42.26±6.84^a	48.93±8.11^a	26.67±7.70^a	23.33±6.67^a
1.0	37.50±8.33^ab	42.26±6.84^a	54.76±13.74^ab	60.83±7.88^b	62.50±14.43^bc	47.22±9.62^b	38.10±5.50^ab
1.5	38.10±5.50^ab	48.81±11.90^ab	62.22±5.44^bc	60.42±12.5^b	59.17±6.87^bc	50.71±15.32^b	46.96±16.72^b
2.0	44.05±7.90^b	54.76±13.74^bc	64.29±14.29^bc	65.00±3.33^bc	64.44±8.89^c	51.39±2.78^b	49.60±5.20^b
2.5	62.50±8.33^c	65.00±3.33^cd	65.00±3.33^bc	69.05±2.75^bc	70.24±2.38^c	57.50±5.00^bc	43.06±11.45^b
3.0	62.02±9.29^c	67.08±6.14^d	71.83±4.56^c	73.61±4.81^c	73.91±3.08^c	69.17±7.26^cd	55.00±5.77^bc
3.5	70.24±2.38^c	73.21±2.06^d	94.44±11.11^d	94.44±11.11^d	88.19±13.68^d	79.46±13.79^d	70.00±6.67^cd
4.0	100.00±0.00^d	100.00±0.00^e	100.00±0.00^d	100.00±0.00^d	93.75±12.50^d	78.33±15.75^d	75.71±17.06^d

组别	动物数	10 min内瘙痒次数
空白组	8	1.50±1.58^∆∆
模型组	8	24.63±3.46^**
溶剂组	8	23.38±5.72^**
阳性对照组	8	19.38±4.27^**∆
1,8-桉叶素组	8	19.88±4.26^**∆

组别	动物数	组胺含量/（μg·L^-1）
空白组	8	18.89±2.18^∆∆
模型组	8	24.07±1.10^**
溶剂组	8	23.23±2.92^*
阳性对照组	8	21.26±2.65^∆
1,8-桉叶素组	8	18.99±2.33^∆∆

组别	动物数	IL-31/（ng·mL^-1）	PAF/（μg·L^-1）	5-HT/（pg·mL^-1）
空白组	8	22.91±0.29^∆∆	79.43±0.81^∆∆	172.86±3.79^∆∆
模型组	8	23.88±0.36^**	86.00±1.30^**	193.81±3.99^**
溶剂组	8	23.85±0.28^**	85.94±0.94^**	192.44±2.00^**
阳性对照组	8	22.57±0.38^∆∆	73.56±1.42^**∆∆	166.81±3.10^*∆∆
1,8-桉叶素组	8	22.57±0.31^∆∆	71.64±1.10^**∆∆	159.58±3.14^**∆∆