川芎提取物美白双靶点活性研究

doi:10.3969/j.issn.1001-1803.2020.04.007

日用化学工业 ›› 2020, Vol. 50 ›› Issue (4): 249-254.doi: 10.3969/j.issn.1001-1803.2020.04.007

川芎提取物美白双靶点活性研究

王勇恒^1,²,梁彦会³,孙培冬^1,²()

^1. 江南大学化学与材料工程学院,江苏无锡 214122
^2. 江南大学合成与生物胶体教育部重点实验室,江苏无锡 214122
^3. 中国香料香精化妆品工业协会,北京 100079

收稿日期:2019-08-16 修回日期:2020-03-20 出版日期:2020-04-22 发布日期:2020-04-24
通讯作者: 孙培冬
作者简介:王勇恒（1993-）男,山东人,硕士研究生,电话：13013625789,E-mail：81421789@qq.com。

Study on the dual-target whitening activities of Ligusticum chuanxiong Hort. extracts

WANG Yong-heng^1,²,LIANG Yan-hui³,SUN Pei-dong^1,²()

^1. School of Chemical & Material Engineering, Jiangnan University, Wuxi, Jiangsu 214122, China
^2. Key Laboratory of Synthetic and Biological Colloids, Ministry of Education, Jiangnan University, Wuxi, Jiangsu 214122, China
^3. China Association of Fragrance Flavour and Cosmetic Industries, Beijing 100079, China

Received:2019-08-16 Revised:2020-03-20 Online:2020-04-22 Published:2020-04-24
Contact: Pei-dong SUN

摘要/Abstract

摘要：

以DPPH自由基清除率和蘑菇酪氨酸酶抑制率为评价指标,对不同溶剂提取的川芎提取物、不同极性部位的活性进行筛选;在细胞水平上分别测定提取物对不同刺激物（α-MSH、Forskolin、IBMX）作用下的黑色素含量、细胞内酪氨酸酶活力的影响;DCFH-DA法测定提取物对UV刺激下细胞内ROS的清除作用。结果表明：川芎的50%乙醇水提取物兼具较高的抗氧化和蘑菇酪氨酸酶抑制作用;对三种刺激物作用下的黑色素含量和细胞内酪氨酸酶活性具有很好的抑制作用;能剂量依赖性地减少UV刺激下的ROS含量。川芎提取物以抑制细胞内酪氨酸酶活性、清除ROS双靶点的方式起到美白作用。

关键词: 川芎, 美白, 双靶点, 黑色素, 抗氧化, 酪氨酸酶

Abstract:

The activities of Ligusticum Chuangxiong Hort. （LH） extracted from different solvents and polar sections were investigated with DPPH free radical scavenging rate and mushroom tyrosinase inhibition rate as evaluation indicators. The effects of LH extracts on melanin contents and intracellular tyrosinase activity under various stimuli （α-MSH、Forskolin、IBMX）were detected in cells. The scavenging ability of LH extracts to intracellular ROS induced by UV was estimated by DCFH-DA. The results display that the 50% ethanol-water extracts of LH possess great antioxidant and mushroom tyrosinase inhibitory effects. The LH extracts can achieve good inhibitory effect on melanin contents and intracellular tyrosinase activity stimulated by three stimuli. The LH extracts eliminate ROS induced by UV in a dose-dependent manner. LH extracts has whitening efficacy by dual targets of inhibiting intracellular tyrosinase and scavenging ROS.

Key words: Ligusticum chuanxiong Hort., whitening, dual-target, melanin, antioxidant, tyrosinase

中图分类号:

TQ658

王勇恒,梁彦会,孙培冬. 川芎提取物美白双靶点活性研究[J]. 日用化学工业, 2020, 50(4): 249-254.

WANG Yong-heng,LIANG Yan-hui,SUN Pei-dong. Study on the dual-target whitening activities of Ligusticum chuanxiong Hort. extracts[J]. China Surfactant Detergent & Cosmetics, 2020, 50(4): 249-254.

图/表 6

图 1

图 2

图 3

图 4

图 5

图 6

参考文献 14

[1]	Manyatja B M, Roumiana V N, Namrita L . Melanogenesis and antityrosinase activity of selected south African plants[J]. Evidence-Based Complementary and Alternative Medicine, 2012, 374017-374022. doi: 10.1155/2012/374017 pmid: 22611429
[2]	Sa H K, Yong D J, Ji Y C , et al. Antioxidant and skin-whitening effects of aerial part of Euphorbia supina Raf. extract[J]. BMC Complementary and Alternative Medicine, 2018 ( 1) : 1-8. doi: 10.1186/s12906-018-2323-5 pmid: 30223806
[3]	Thouria B, Myra O V, Chemseddoha G , et al. Depigmenting effect of argan press-cake extract through the down-regulation of Mitf and melanogenic enzymes expression in B16 murine melanoma cells[J]. Cytotechnology, 2018,70(6) : 1389-1397. doi: 10.1007/s10616-018-0232-6 pmid: 29946948
[4]	Yun M J, Won K O, Tien L T , et al. Aglycone of Rh4 inhibits melanin synjournal in B16 melanoma cells: Possible involvement of the protein Kinase A pathway[J]. Biosci Biotechnol Biochem, 2013,77(1) : 119-125. doi: 10.1271/bbb.120602 pmid: 23291754
[5]	Ji Y Y, Eunmiri R, Jong K S , et al. Effect of saucerneol D on melanin production in cAMP-elevated melanocytes[J]. Archives of Pharmacal Research, 2011,34(8) : 1339-1345. doi: 10.1007/s12272-011-0814-8 pmid: 21910056
[6]	Jeong O L, Eunji K J H K, Yo H H, et al. Antimelanogenesis and skin-protective activities of Panax ginseng calyx ethanol extract[J]. Journal of Ginseng Research, 2018,42(3) : 389-399. doi: 10.1016/j.jgr.2018.02.007 pmid: 29983620
[7]	Chinese Pharmacopoeia Commission. Chinese pharmacopoeia Ⅰ [M]. Beijing: Chemical Industry Press, 2005: 28.
[8]	Zhang Zhiping . Extraction and separation skin-whitening agent from Rhizoma Chuanxiong and its application in cosmetics[D]. Guangdong: Guangdong Pharmaceutical College, 2014.
[9]	Deng Xiangqing, Gong Shengzhao, Jie Xiangyang . Study on the inhibition of tyrosinase activity by Ligusticum chuanxiong Hort. extract[J]. Journal of Chinese Medicinal Materials, 2007,30(4) : 469-471.
[10]	Tie Y J, Kandasamy S, Myeong H W . In vitro and in vivo antioxidant properties of water and methanol extracts of linden bee pollen[J]. Biocatalysis and Agricultural Biotechnology, 2018,13:186-189.
[11]	Mayuree K, Nattaya L, Sarin T , et al. Rice panicles: New promising unconventional cereal product for health benefits[J]. Journal of Cereal Science, 2015,66:10-17.
[12]	Shang T W, Wei C C, Chen H , et al. Antimelanogenic effect of Urolithin A and Urolithin B, the colonic metabolites of ellagic acid, in B16 melanoma cells[J]. Journal of Agricultural and Food Chemistry, 2017,65(32) : 6870-6876. doi: 10.1021/acs.jafc.7b02442 pmid: 28726389
[13]	Jeung H K, Hyoung T K, Ha Y Y , et al. Effect of xanthohumol on melanogenesis in B16 melanoma cells[J]. Experimental and Molecular Medicine, 2008,40(3) : 313-319. doi: 10.3858/emm.2008.40.3.313 pmid: 18587269
[14]	Bonggi L, Kyoung M M, Bong S L , et al. Swertiajaponin inhibits skin pigmentation by dual mechanisms to suppress tyrosinase[J]. Oncotarget, 2017,8(56) : 95530-95541. doi: 10.18632/oncotarget.20913 pmid: 29221146

[1]	李瑶瑶. 异橙黄酮的抗衰老及抗氧化功效研究[J]. 日用化学工业（中英文）, 2024, 54(3): 313-319.
[2]	肖雪, 张慧荣, 李硕雯, 李孙华, 郭苗苗, 李丽. 滨海刺芹愈伤组织提取物中4种多酚含量测定及抗氧化、抗炎活性研究[J]. 日用化学工业（中英文）, 2024, 54(1): 57-64.
[3]	王安娜, 方梦婕, 唐超, 岳天翔. 丹参素对UVB诱导的皮肤光老化小鼠的保护作用和抗氧化机制研究[J]. 日用化学工业（中英文）, 2024, 54(1): 65-72.
[4]	李宁, 李恩念, 陈红波, 程芳, 邹衡芳, 陈鸿鹏. 祛斑美白类化妆品功效成分的研究现状[J]. 日用化学工业（中英文）, 2024, 54(1): 80-89.
[5]	陈丽媛, 高合意, 唐金晶, 刘作华, 刘仁龙. 锰化合物抗氧化及保护头发光损伤功效评价[J]. 日用化学工业（中英文）, 2023, 53(9): 1044-1050.
[6]	李学军,米锐,王林美,李亚洁,陈雪,都兴范. 柞蚕丝素肽的抗氧化及美白功效评价[J]. 日用化学工业（中英文）, 2023, 53(8): 891-898.
[7]	韩雨迪, 金莉英, 孙熙浛, 林长青, 崔承弼. 人参红景天乳液的制作及其抗氧化、美白作用研究[J]. 日用化学工业（中英文）, 2023, 53(6): 665-672.
[8]	亓国锋, 李刚刚, 安晏. 酶水解法制备黄芪素及应用研究[J]. 日用化学工业（中英文）, 2023, 53(5): 544-550.
[9]	郭芳钰, 韩婷婷, 王晓娜, 陈玉荣, 王晓梅, 杨素珍. 中药双向发酵液制备及其抗衰老、保湿、美白功效研究[J]. 日用化学工业（中英文）, 2023, 53(5): 523-531.
[10]	张慧荣, 郭苗苗, 陈晨, 潘乾印, 张影, 李丽. 三种豆类植物多肽的制备工艺及其活性研究[J]. 日用化学工业（中英文）, 2023, 53(4): 423-429.
[11]	郝晴, 吴二敏, 毕永贤, 刘金俊, 孔德承, 成志伟. 莲子心总黄酮多元醇提取工艺筛选及其性能研究[J]. 日用化学工业（中英文）, 2023, 53(4): 398-407.
[12]	任洁洁. 逆境胁迫处理后山楂叶总黄酮含量变化及对心肌缺血性大鼠分子机制的研究[J]. 日用化学工业（中英文）, 2023, 53(4): 430-436.
[13]	徐雅妮, 杨凯业, 朱溶滔, 刘光荣, 韩萍, 杜志云. 复合多肽延缓小鼠皮肤衰老的功效研究[J]. 日用化学工业（中英文）, 2023, 53(12): 1421-1428.
[14]	邓梦洁, 易国斌, 吕冉, 柳亚锋, 叶大威, 陈佳志. 阿魏酸纳米醇质体的制备、表征及体外经皮吸收研究[J]. 日用化学工业（中英文）, 2023, 53(11): 1285-1292.
[15]	周妍, 韩蕊娜. 红景天提取物的护肤功效及安全性评价研究[J]. 日用化学工业（中英文）, 2023, 53(10): 1180-1185.

川芎提取物美白双靶点活性研究

Study on the dual-target whitening activities of Ligusticum chuanxiong Hort. extracts

RichHTML

PDF (PC)

赞

可视化

摘要/Abstract

引用本文

使用本文

图/表 6

参考文献 14

相关文章 15

Metrics

本文评价

推荐阅读 10